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GRLF1 antibody (pTyr1105)

GRLF1 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, ICC, IF Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6256617
  • Target See all GRLF1 Antibodies
    GRLF1 (Glucocorticoid Receptor DNA Binding Factor 1 (GRLF1))
    Binding Specificity
    • 13
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pTyr1105
    Reactivity
    • 25
    • 20
    • 20
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 25
    • 1
    Rabbit
    Clonality
    • 25
    • 1
    Polyclonal
    Conjugate
    • 19
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This GRLF1 antibody is un-conjugated
    Application
    • 15
    • 12
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
    Specificity
    Phospho-GRF-1 (Tyr1105) Antibody detects endogenous levels of GRF-1 only when phosphorylated at Tyrosine 1105.
    Predicted Reactivity
    Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus
    Purification
    The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
    Immunogen
    A synthesized peptide derived from human GRF-1 around the phosphorylation site of Tyr1105.
    Isotype
    IgG
  • Application Notes
    WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store at -20 °C. Stable for 12 months from date of receipt.
    Expiry Date
    12 months
  • Target
    GRLF1 (Glucocorticoid Receptor DNA Binding Factor 1 (GRLF1))
    Alternative Name
    ARHGAP35 (GRLF1 Products)
    Synonyms
    GRF-1 antibody, GRLF1 antibody, P190-A antibody, P190A antibody, p190ARhoGAP antibody, p190RhoGAP antibody, 6430596G11Rik antibody, AI841135 antibody, Arhgap35 antibody, mKIAA1722 antibody, p190A antibody, Grlf1 antibody, p190-A antibody, arhgap35 antibody, grf-1 antibody, grlf1 antibody, p190-a antibody, p190a antibody, p190arhogap antibody, p190rhogap antibody, Rho GTPase activating protein 35 antibody, Rho GTPase activating protein 35 L homeolog antibody, ARHGAP35 antibody, Arhgap35 antibody, arhgap35.L antibody
    Background

    Description: Rho GTPase-activating protein (GAP) (PubMed:19673492, PubMed:28894085). Binds several acidic phospholipids which inhibits the Rho GAP activity to promote the Rac GAP activity (PubMed:19673492). This binding is inhibited by phosphorylation by PRKCA (PubMed:19673492). Involved in cell differentiation as well as cell adhesion and migration, plays an important role in retinal tissue morphogenesis, neural tube fusion, midline fusion of the cerebral hemispheres and mammary gland branching morphogenesis (By similarity). Transduces signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP) (By similarity). Transduces SRC-dependent signals from cell-surface adhesion molecules, such as laminin, to promote neurite outgrowth. Regulates axon outgrowth, guidance and fasciculation (By similarity). Modulates Rho GTPase-dependent F-actin polymerization, organization and assembly, is involved in polarized cell migration and in the positive regulation of ciliogenesis and cilia elongation (By similarity). During mammary gland development, is required in both the epithelial and stromal compartments for ductal outgrowth (By similarity). Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'-GAGAAAAGAAACTGGAGAAACTC-3', this function is however unclear and would need additional experimental evidences (PubMed:1894621).

    Gene: ARHGAP35

    Molecular Weight
    155kDa
    Gene ID
    2909
    UniProt
    Q9NRY4
    Pathways
    Tube Formation
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