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Iba1 antibody (AA 51-147)

IBA1 Reactivity: Human, Rat, Mouse WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN685477
  • Target See all Iba1 (IBA1) Antibodies
    Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))
    Binding Specificity
    • 16
    • 15
    • 15
    • 10
    • 9
    • 8
    • 8
    • 7
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 51-147
    Reactivity
    • 88
    • 54
    • 47
    • 2
    • 1
    Human, Rat, Mouse
    Host
    • 76
    • 32
    • 6
    • 1
    Rabbit
    Clonality
    • 78
    • 37
    Polyclonal
    Conjugate
    • 55
    • 18
    • 13
    • 7
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This Iba1 antibody is un-conjugated
    Application
    • 79
    • 47
    • 42
    • 21
    • 20
    • 13
    • 13
    • 13
    • 12
    • 7
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Cross-Reactivity
    Human, Mouse, Rat
    Predicted Reactivity
    Pig
    Purification
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic peptide derived from human AIF1
    Isotype
    IgG
    Top Product
    Discover our top product IBA1 Primary Antibody
  • Application Notes
    WB 1:300-5000
    ELISA 1:500-1000
    FCM 1:20-100
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    Restrictions
    For Research Use only
  • Validation #029613 (Immunofluorescence)
    'Independent Validation' Badge
    by
    Reveal Biosciences
    No.
    #029613
    Date
    02/21/2014
    Antigen
    Lot Number
    980993W
    Method validated
    Immunofluorescence
    Positive Control
    Mouse spleen
    Negative Control
    Mouse duodenum
    Notes
    Strong signal was observed in the positive control tissue, while no signal was observed in the negative control tissue.
    'Independent Validation' Badge
    Validation Images
    Full Methods
    Primary Antibody
    • Antibody: AIF1
    • Catalog number: ABIN685477
    • Lot number: 980993W
    Secondary Antibody
    • Antibody: Alexa Fluor 546 Donkey Anti Rabbit
    • Lot number: 1218269
    Full Protocol
    • Frozen, OCT embedded tissues were cut 5 µm and mounted on positive charged slides.
    • Sections were air dried for 30 min prior to fix in 10% neutral buffered formalin for 10 min.
    • Fixed slides were rinsed three times in PBS for 5 min each at RT.
    • Sections were blocked in 1 X PBS / 3% donkey serum / 0.1% Triton X-100 for 10 min at RT.
    • Sections were incubated with primary antibody diluted 1:100 in Universal Antibody Dilution Buffer (Electron Microscopy Sciences, 25886-05) overnight at 4°C.
    • Sections were rinsed three times in PBS for 5 min each at RT.
    • Sections were incubated with secondary antibody diluted 1:200 in Universal Antibody Dilution Buffer (Electron Microscopy Sciences, 25886-05) for 120 min at RT in dark.
    • Sections were mounted on slides with FluoroGel II with DAPI (Electron Microscopy Sciences).
    • Stained sections were imaged with a 3D Histech Pannoramic SCAN 150.
    Experimental Notes
    None
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Expiry Date
    12 months
  • Ko, Shie, Lin, Chen, Yao, Chen: "Biodegradable Bisvinyl Sulfonemethyl-crosslinked Gelatin Conduit Promotes Regeneration after Peripheral Nerve Injury in Adult Rats." in: Scientific reports, Vol. 7, Issue 1, pp. 17489, (2018) (PubMed).

    Hsu, Yao, Hsu, Lin, Chen, Chen: "Effects of Taxol on Regeneration in a Rat Sciatic Nerve Transection Model." in: Scientific reports, Vol. 7, pp. 42280, (2017) (PubMed).

    Fu, Xia, Liu, Wang, Wang, Shi, Song, Song, Song et al.: "The acute exposure of tetrachloro-p-benzoquinone (a.k.a. chloranil) triggers inflammation and neurological dysfunction via Toll-like receptor 4 signaling: The protective role of melatonin ..." in: Toxicology, Vol. 381, pp. 39-50, (2017) (PubMed).

    Hu, Kodithuwakku, Zhou, Li, Han, Fang, Liu, Li: "Levo-Corydalmine Alleviates Neuropathic Cancer Pain Induced by Tumor Compression via the CCL2/CCR2 Pathway." in: Molecules (Basel, Switzerland), Vol. 22, Issue 6, (2017) (PubMed).

    Furukawa, Nagaike, Ozaki: "Databases for technical aspects of immunohistochemistry." in: Journal of toxicologic pathology, Vol. 30, Issue 1, pp. 79-107, (2017) (PubMed).

    Kouam, Yuan, Njayou, He, Tsayem, Oladejo, Song, Moundipa, Gao: "Induction of Mkp-1 and Nuclear Translocation of Nrf2 by Limonoids from Khaya grandifoliola C.DC Protect L-02 Hepatocytes against Acetaminophen-Induced Hepatotoxicity." in: Frontiers in pharmacology, Vol. 8, pp. 653, (2017) (PubMed).

    Ogawa, Onozato, Okuhara, Nagasawa, Tamura, Hayashi: "Spontaneous cutaneous soft tissue sarcoma with differentiation into fibroblasts in a Sprague-Dawley rat." in: Journal of toxicologic pathology, Vol. 29, Issue 2, pp. 119-24, (2016) (PubMed).

  • Target
    Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))
    Alternative Name
    AIF1 (IBA1 Products)
    Synonyms
    AIF-1 antibody, IBA1 antibody, IRT-1 antibody, IRT1 antibody, AI607846 antibody, D17H6S50E antibody, G1 antibody, Iba1 antibody, Bart1 antibody, iba1 antibody, mrf-1 antibody, fa04b11 antibody, wu:fa04b11 antibody, AIF antibody, AIF1 antibody, aif1 antibody, allograft inflammatory factor 1 antibody, AIF1 antibody, Aif1 antibody, aif1 antibody
    Background

    Synonyms: IBA1, IRT1, AIF-1, IRT-1, Allograft inflammatory factor 1, Ionized calcium-binding adapter molecule 1, Protein G1, AIF1, G1

    Background: Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.

    Gene ID
    199
    UniProt
    P55008
    Pathways
    Smooth Muscle Cell Migration
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