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Actin antibody (C-Term)

Name: Actin antibody (C-Term)
SKU: ABIN3043519
Price: 370 USD
Availability: InStock

ACTA1 Reactivity: Human, Mouse, Rat WB, IHC (p) Host: Rabbit Polyclonal unconjugated

Western Blotting (WB) image for anti-Actin, alpha 1, Skeletal Muscle (ACTA1) (AA 277-308), (C-Term) antibody (ABIN3043519)

IHC (p)

4 images

(64 references)

Catalog No. ABIN3043519

Datasheet as PDF

Target See all Actin (ACTA1) Antibodies

Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))
Binding Specificity
18

9

8

7

7

6

5

1

1

1

1

1

1

1
AA 277-308, C-Term
Reactivity
95

43

42

17

12

10

9

8

6

6

5

5

4

3

3

2

2

2

1

1

1

1

1

1
Human, Mouse, Rat
Host
80

31
Rabbit
Compatible Secondaries
Clonality
75

34
Polyclonal
Conjugate
62

12

12

10

5

4

1

1

1

1

1

1
This Actin antibody is un-conjugated
Application
90

57

40

28

27

24

10

9

7

2

1

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose

Rabbit IgG polyclonal antibody for Actin, alpha skeletal muscle(ACTA1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence

HETTYNSIMK CDIDIRKDLY ANNVMSGGTT MY

Cross-Reactivity (Details)

No cross reactivity with other proteins.

Characteristics

Rabbit IgG polyclonal antibody for Actin, alpha skeletal muscle(ACTA1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: actin, alpha 1, skeletal muscle
Protein Name: Actin, alpha skeletal muscle

Purification

Immunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Actin (277-308aa HETTYNSIMKCDIDIRKDLYANNVMSGGTTMY), identical to the related mouse and rat sequences.

Isotype

IgG
anti-Actin, alpha 1, Skeletal Muscle (ACTA1) antibody
ACTA1 Reactivity: Human, Rat, Rabbit, Zebrafish (Danio rerio), Pig, Chicken, Monkey WB, ELISA, IHC, IHC (p), IHC (fro), IEM Host: Mouse Monoclonal HHF35 unconjugated

anti-Actin, alpha 1, Skeletal Muscle (ACTA1) (AA 346-375), (C-Term) antibody
ACTA1 Reactivity: Human WB, IHC (p), IF, FACS Host: Rabbit Polyclonal RB35391 unconjugated

anti-Actin, alpha 1, Skeletal Muscle (ACTA1) (AA 346-375) antibody
ACTA1 Reactivity: Human WB, ELISA, IHC, IF, FACS Host: Rabbit Polyclonal unconjugated

anti-Actin, alpha 1, Skeletal Muscle (ACTA1) (AA 3-377) antibody
ACTA1 Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-Actin, alpha 1, Skeletal Muscle (ACTA1) antibody
ACTA1 Reactivity: Human WB, ELISA, IHC, IF, FACS Host: Rabbit Monoclonal 25E3 unconjugated Recombinant Antibody

anti-Actin, alpha 1, Skeletal Muscle (ACTA1) (AA 1-129) antibody
ACTA1 Reactivity: Human, Zebrafish (Danio rerio), Fish WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Actin, alpha 1, Skeletal Muscle (ACTA1) (AA 7-377) antibody
ACTA1 Reactivity: Human ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Actin, alpha 1, Skeletal Muscle (ACTA1) (N-Term) antibody
ACTA1 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Actin, alpha 1, Skeletal Muscle (ACTA1) antibody
ACTA1 Reactivity: Human, Mouse, Rat WB, IP Host: Mouse Monoclonal 4B11 unconjugated

anti-Actin, alpha 1, Skeletal Muscle (ACTA1) antibody
ACTA1 Reactivity: Human, Mouse, Rat, Rabbit, Chicken, Dog, Non-Human Primate, Cat WB, IHC (p), ICC, IHC (fro) Host: Mouse Monoclonal HHF35 unconjugated

Application Notes

WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions

For Research Use only
Format

Lyophilized

Reconstitution

Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration

500 μg/mL

Buffer

Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freezing and thawing.

Storage

4 °C/-20 °C

Storage Comment

At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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Target

Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Alternative Name

ACTA1 (ACTA1 Products)

Synonyms

ACTA antibody, ASMA antibody, CFTD antibody, CFTD1 antibody, CFTDM antibody, MPFD antibody, NEM1 antibody, NEM2 antibody, NEM3 antibody, acta antibody, asma antibody, cftd antibody, cftd1 antibody, cftdm antibody, mpfd antibody, nem1 antibody, AA959943 antibody, Acta-2 antibody, Acts antibody, Actsk-1 antibody, actc antibody, actc1 antibody, cfk antibody, hm:zeh0631 antibody, zeh0631 antibody, acta1 antibody, hm:zeh0293 antibody, zgc:112098 antibody, actin, alpha 1, skeletal muscle antibody, actin, alpha 1, skeletal muscle L homeolog antibody, actin, alpha 1b, skeletal muscle antibody, actin, muscle antibody, actin, alpha skeletal muscle antibody, actin, alpha 1a, skeletal muscle antibody, ACTA1 antibody, acta1.L antibody, acta1 antibody, Acta1 antibody, acta1b antibody, LOC100533357 antibody, LOC109479779 antibody, LOC109108280 antibody, acta1a antibody

Background

Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90 % sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin, two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins, this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.

Synonyms: ACTA antibody|ACTA1 antibody|Actin alpha skeletal muscle antibody|Actin antibody|actin, alpha 1, skeletal muscle 1 antibody|actin, alpha 1, skeletal muscle antibody|Actin, alpha skeletal muscle antibody|actina antibody|actine antibody|ACTS_HUMAN antibody|aktin antibody|Alpha Actin 1 antibody|Alpha skeletal muscle Actin antibody|alpha skeletal muscle antibody|alpha-actin antibody|Alpha-actin-1 antibody|ASMA antibody|CFTD antibody|CFTD1antibody|CFTDM antibody|MPFD antibody|NEM1 antibody|NEM2 antibody|NEM3 antibody|nemaline myopathy type 3 antibody

Gene ID

58

UniProt

P68133

Pathways

Caspase Cascade in Apoptosis, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development