T-Bet antibody (Internal Region)
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- Target See all T-Bet Antibodies
- T-Bet
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Binding Specificity
- Internal Region
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This T-Bet antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
- Specificity
- Tbx21 Antibody detects endogenous levels of total tbx21.
- Predicted Reactivity
- Pig,Bovine,Sheep,Rabbit,Dog
- Purification
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- Immunogen
- A synthesized peptide derived from human tbx21, corresponding to a region within the internal amino acids.
- Isotype
- IgG
- Top Product
- Discover our top product T-Bet Primary Antibody
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anti-T-Bet (AA 241-493) antibody
Reactivity: Rat WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-T-Bet (AA 338-387) antibodyReactivity: Human, Rat, Cow, Dog, Pig, Rabbit, Guinea Pig, Monkey, Bat WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-T-Bet (Middle Region) antibodyReactivity: Human, Mouse, Rat, Cow, Dog, Pig, Rabbit, Guinea Pig WB, IHC Host: Rabbit Polyclonal unconjugated
anti-T-Bet (AA 326-535) antibodyReactivity: Human WB Host: Rabbit Polyclonal unconjugated
anti-T-Bet (AA 160-399) antibodyReactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-T-Bet antibodyReactivity: Human, Mouse, Rat WB, IF (p), IHC (p) Host: Rabbit Polyclonal unconjugated
anti-T-Bet (AA 387-486) antibodyReactivity: Human WB, ELISA Host: Mouse Monoclonal 4F8 unconjugated
anti-T-Bet (AA 400-535) antibodyReactivity: Human WB, ELISA Host: Mouse Monoclonal 2F10 unconjugated
anti-T-Bet (AA 82-395) antibodyReactivity: Human WB, ELISA Host: Rabbit Polyclonal unconjugated
anti-T-Bet (C-Term) antibodyReactivity: Human, Mouse WB, ELISA, IHC, IHC (p), IF Host: Rabbit Polyclonal unconjugated
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- Application Notes
- WB 1:1000-3000, IHC 1:200, IF1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20 °C. Stable for 12 months from date of receipt.
- Expiry Date
- 12 months
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- Target
- T-Bet
- Alternative Name
- TBX21 (T-Bet Products)
- Synonyms
- T-PET antibody, T-bet antibody, TBET antibody, TBLYM antibody, TBT1 antibody, Tbet antibody, Tblym antibody, T-box 21 antibody, TBX21 antibody, Tbx21 antibody
- Background
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Description: Lineage-defining transcription factor which initiates Th1 lineage development from naive Th precursor cells both by activating Th1 genetic programs and by repressing the opposing Th2 and Th17 genetic programs (PubMed:10761931). Activates transcription of a set of genes important for Th1 cell function, including those encoding IFN-gamma and the chemokine receptor CXCR3. Activates IFNG and CXCR3 genes in part by recruiting chromatin remodeling complexes including KDM6B, a SMARCA4-containing SWI/SNF-complex, and an H3K4me2-methyltransferase complex to their promoters and all of these complexes serve to establish a more permissive chromatin state conducive with transcriptional activation (By similarity). Can activate Th1 genes also via recruitment of Mediator complex and P-TEFb (composed of CDK9 and CCNT1/cyclin-T1) in the form of the super elongation complex (SEC) to super-enhancers and associated genes in activated Th1 cells (PubMed:27292648). Inhibits the Th17 cell lineage commitment by blocking RUNX1-mediated transactivation of Th17 cell-specific transcriptinal regulator RORC. Inhibits the Th2 cell lineage commitment by suppressing the production of Th2 cytokines, such as IL-4, IL-5, and IL- 13, via repression of transcriptional regulators GATA3 and NFATC2. Protects Th1 cells from amplifying aberrant type-I IFN response in an IFN-gamma abundant microenvironment by acting as a repressor of type-I IFN transcription factors and type-I IFN-stimulated genes. Acts as a regulator of antiviral B-cell responses, controls chronic viral infection by promoting the antiviral antibody IgG2a isotype switching and via regulation of a broad antiviral gene expression program (By similarity).
Gene: TBX21
- Molecular Weight
- 58 kDa
- Gene ID
- 30009
- UniProt
- Q9UL17
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