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HIST1H1C antibody (acLys96)

Name: HIST1H1C antibody (acLys96)
Brand: antibodies-online
SKU: ABIN7139207
Price: 350.9 USD
Availability: InStock

HIST1H1C Reactivity: Human ELISA, WB, IF, ChIP, ICC, IP Host: Rabbit Polyclonal unconjugated

Chromatin Immunoprecipitation Hela (4*10 6 , treated with 30 mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8 μg anti-HIST1H1C (ABIN7139207) or a control normal rabbit IgG.

IHC

Immunoprecipitating HIST1H1C in Hela whole cell lysate (treated with 30 mM sodium butyrate for 4h) Lane 1: Rabbit control IgG instead of ABIN7139207 in Hela whole cell lysate (treated with 30 mM sodium butyrate for 4h).

Western Blot Detected samples: Hela whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate, Untreated (-) or treated (+) with 30 mM sodium butyrate for 4h All lanes: HIST1H1C antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 22 kDa Observed band size: 22 kDa

Immunocytochemistry analysis of Hela cells using ABIN7139207 at dilution of 1:100

ICC

Immunofluorescent analysis of Hela cells (sodium butyrate, 30 mM, 4h) using ABIN7139207 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

5 images

Catalog No. ABIN7139207

Datasheet as PDF

Target See all HIST1H1C Antibodies

HIST1H1C (Histone Cluster 1, H1c (HIST1H1C))
Binding Specificity
7

6

6

6

5

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
acLys96
Reactivity
78

18

18

1

1

1

1

1
Human
Host
78
Rabbit
Compatible Secondaries
Clonality
68

10
Polyclonal
Conjugate
51

4

4

3

2

2

2

2

2

2

2

2
This HIST1H1C antibody is un-conjugated
Application
56

37

21

15

13

10

9

3

2
ELISA, Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Cross-Reactivity

Human

Purification

Antigen Affinity Purified

Immunogen

Peptide sequence around site of Acetyl-Lys (96) derived from Human Histone H1.2

Isotype

IgG
anti-Histone Cluster 1, H1c (HIST1H1C) (2meLys45) antibody
HIST1H1C Reactivity: Human ELISA, WB, ChIP, ICC, IP Host: Rabbit Polyclonal unconjugated

anti-Histone Cluster 1, H1c (HIST1H1C) (AA 2-213) antibody
HIST1H1C Reactivity: Human ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Histone Cluster 1, H1c (HIST1H1C) (meLys96) antibody
HIST1H1C Reactivity: Human ELISA, WB, IF, ChIP Host: Rabbit Polyclonal unconjugated

anti-Histone Cluster 1, H1c (HIST1H1C) (meLys45) antibody
HIST1H1C Reactivity: Human ELISA, WB, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Histone Cluster 1, H1c (HIST1H1C) (meLys186) antibody
HIST1H1C Reactivity: Human ELISA, WB, IF, ChIP Host: Rabbit Polyclonal unconjugated

anti-Histone Cluster 1, H1c (HIST1H1C) (acLys84) antibody
HIST1H1C Reactivity: Human ELISA, WB, ChIP, ICC Host: Rabbit Polyclonal unconjugated

anti-Histone Cluster 1, H1c (HIST1H1C) (acLys62) antibody
HIST1H1C Reactivity: Human ELISA, IF, ChIP, ICC Host: Rabbit Polyclonal unconjugated

anti-Histone Cluster 1, H1c (HIST1H1C) (acLys16) antibody
HIST1H1C Reactivity: Human ELISA, IF, ChIP, ICC Host: Rabbit Polyclonal unconjugated

anti-Histone Cluster 1, H1c (HIST1H1C) (N-Term) antibody
HIST1H1C Reactivity: Human, Mouse IHC, WB Host: Rabbit Polyclonal unconjugated

anti-Histone Cluster 1, H1c (HIST1H1C) antibody
HIST1H1C Reactivity: Human WB, IF Host: Rabbit Monoclonal unconjugated

Application Notes

Recommended dilution: WB:1:1000-1:5000, ICC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000,

Restrictions

For Research Use only
Format

Liquid

Buffer

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, pH 7.4

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C,-80 °C

Storage Comment

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target

HIST1H1C (Histone Cluster 1, H1c (HIST1H1C))

Alternative Name

HIST1H1C (HIST1H1C Products)

Synonyms

H1.2 antibody, H1C antibody, H1F2 antibody, H1s-1 antibody, 0610008C09Rik antibody, H1c antibody, H1f2 antibody, H1var1 antibody, His1a antibody, histone cluster 1 H1 family member c antibody, histone cluster 1, H1c antibody, HIST1H1C antibody, Hist1h1c antibody

Background

Background: Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
Aliases: H1 histone family member 2 antibody, H1.a antibody, H12_HUMAN antibody, H1F2 antibody, H1s-1 antibody, HIST1H1C antibody, Histone 1 H1c antibody, Histone cluster 1 H1c antibody, Histone H1.2 antibody, Histone H1c antibody, Histone H1d antibody, Histone H1s-1 antibody, MGC3992 antibody

UniProt

P16403