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H2AFZ antibody (acLys4, acLys7, acLys11)

Name: H2AFZ antibody (acLys4, acLys7, acLys11)
SKU: ABIN5773877
Price: 944 USD
Availability: InStock

H2AFZ Reactivity: Human, Mouse WB, ELISA, IF, ChIP, ChIP-seq, DB Host: Rabbit Polyclonal unconjugated

Western Blot analysis of (1) 25 ug whole cell extracts of Hela cells, (2) 1 ug of recombinant histone H2A, (3) 1 ug of recombinant histone H2B, (4) 1 ug of recombinant histone H3, (5) 1 ug of recombinant histone H4.

ChIP assays were performed using human K562 cells.

Immunofluorescent staining of Hela cell line with antibody followed by an anti-rabbit antibody conjugated to Alexa488 (left).

ELISA

ChIP assays were performed using human HeLa cells.

ChIP was performed on sheared chromatin from 100,000 K562 cells using antibody.

Cross reactivity test using the Histone H2AZ antibody.

7 images

Catalog No. ABIN5773877

Datasheet as PDF

Target See all H2AFZ Antibodies

H2AFZ (H2A Histone Family, Member Z (H2AFZ))
Binding Specificity
16

15

9

8

8

7

5

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1
acLys4, acLys7, acLys11
Reactivity
95

32

30

5

5

5

5

4

4

3

3

3

3

2

1

1

1
Human, Mouse
Host
98

1
Rabbit
Compatible Secondaries
Clonality
77

22
Polyclonal
Conjugate
60

5

4

3

3

3

3

3

3

3

2

2

1

1

1

1

1
This H2AFZ antibody is un-conjugated
Application
61

46

27

18

16

13

13

12

11

6

6

6

3

3

3

2

1

1

1

1

1
Western Blotting (WB), ELISA, Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq), Dot Blot (DB)

Purpose

Rabbit polyclonal antibody raised against synthetic peptide of Histone H2AZ.

Cross-Reactivity

Human, Mouse

Immunogen

A synthetic peptide (conjugated with KLH) corresponding to Histone HAZ, acetylated at lysines 4, 7 and 11.
anti-H2A Histone Family, Member Z (H2AFZ) (acLys7) antibody
H2AFZ Reactivity: Human WB, ELISA, IF, ICC, ChIP, IP Host: Rabbit Polyclonal unconjugated

anti-H2A Histone Family, Member Z (H2AFZ) (acLys4) antibody
H2AFZ Reactivity: Human WB, ELISA, IF, ICC, ChIP, IP Host: Rabbit Polyclonal unconjugated

anti-H2A Histone Family, Member Z (H2AFZ) (C-Term) antibody
H2AFZ Reactivity: Human WB, IF, ICC, ChIP, IP, IHC (p), IHC (wm) Host: Rabbit Polyclonal unconjugated

anti-H2A Histone Family, Member Z (H2AFZ) (C-Term) antibody
H2AFZ Reactivity: Human WB, IF, ICC, ChIP, ChIP-seq, DB Host: Rabbit Polyclonal unconjugated

anti-H2A Histone Family, Member Z (H2AFZ) (acLys11), (acLys4), (acLys7) antibody
H2AFZ Reactivity: Human ELISA, IF, ChIP, ChIP-seq, DB Host: Rabbit Polyclonal unconjugated

anti-H2A Histone Family, Member Z (H2AFZ) (C-Term) antibody
H2AFZ Reactivity: Human, Mouse WB, ELISA, IF, ChIP, ChIP-seq Host: Rabbit Polyclonal unconjugated

anti-H2A Histone Family, Member Z (H2AFZ) (N-Term) antibody
H2AFZ Reactivity: Human WB, IF, ICC, IHC (p), IHC (wm) Host: Rabbit Polyclonal unconjugated

anti-H2A Histone Family, Member Z (H2AFZ) (C-Term) antibody
H2AFZ Reactivity: Human WB, IF, ChIP, ChIP-seq, DB, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-H2A Histone Family, Member Z (H2AFZ) (meLys4) antibody
H2AFZ Reactivity: Human ELISA, IF, ICC, ChIP Host: Rabbit Polyclonal unconjugated

anti-H2A Histone Family, Member Z (H2AFZ) (acLys11) antibody
H2AFZ Reactivity: Human WB, ELISA, IF, ChIP Host: Rabbit Polyclonal unconjugated

Application Notes

ELISA (1:500)
Western Blot (1:1000)
ChIP (1 µL/IP)
Dot Blot (1:20000)
Immunofluorescence (1:500)
The optimal working dilution should be determined by the end user.

Restrictions

For Research Use only
Format

Liquid

Buffer

In PBS (0.05 % sodium azide, 0.05 % proclin 300).

Preservative

ProClin, Sodium azide

Precaution of Use

This product contains ProClin and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage

-20 °C,-80 °C

Storage Comment

Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.
Target

H2AFZ (H2A Histone Family, Member Z (H2AFZ))

Alternative Name

H2AFZ (H2AFZ Products)

Synonyms

H2A.Z-1 antibody, H2A.z antibody, H2A/z antibody, H2AZ antibody, H2A.Z antibody, H2a.z-1 antibody, H2AFV antibody, H2Az antibody, XH2AZ antibody, h2a.zl1 antibody, h2a.zl2 antibody, H2A histone family member Z antibody, H2A histone family, member Z antibody, H2A histone family member Z L homeolog antibody, histone H2A.Z antibody, H2AFZ antibody, H2afz antibody, h2afz.L antibody

Background

Full Gene Name: H2A histone family, member Z
Synonyms: H2A.z,H2A/z,H2AZ,MGC117173

Gene ID

3015

Pathways

Telomere Maintenance