DAP Kinase 1 antibody (AA 1331-1430)
(1 reference)-
- Target See all DAP Kinase 1 (DAPK1) Antibodies
- DAP Kinase 1 (DAPK1) (Death-Associated Protein Kinase 1 (DAPK1))
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Binding Specificity
- AA 1331-1430
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This DAP Kinase 1 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Predicted Reactivity
- Human,Mouse,Rat
- Purification
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic peptide derived from human DAPK1
- Isotype
- IgG
- Top Product
- Discover our top product DAPK1 Primary Antibody
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anti-Death-Associated Protein Kinase 1 (DAPK1) (AA 1360-1389), (C-Term) antibody
DAPK1 Reactivity: Human, Mouse WB, IF, IHC (p) Host: Rabbit Polyclonal RB3033 unconjugated
anti-Death-Associated Protein Kinase 1 (DAPK1) (Internal Region) antibodyDAPK1 Reactivity: Human, Mouse, Rat WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated
anti-Death-Associated Protein Kinase 1 (DAPK1) (AA 1211-1310) antibodyDAPK1 Reactivity: Human ELISA, PLA Host: Mouse Monoclonal 2E7 unconjugated
anti-Death-Associated Protein Kinase 1 (DAPK1) antibodyDAPK1 Reactivity: Human, Mouse WB, IHC, IF, IC Host: Rabbit Polyclonal unconjugated
anti-Death-Associated Protein Kinase 1 (DAPK1) antibodyDAPK1 Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Death-Associated Protein Kinase 1 (DAPK1) (Ser296) antibodyDAPK1 Reactivity: Human, Mouse WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Death-Associated Protein Kinase 1 (DAPK1) antibodyDAPK1 Reactivity: Human, Mouse, Rat WB Host: Rabbit Polyclonal unconjugated
anti-Death-Associated Protein Kinase 1 (DAPK1) (N-Term) antibodyDAPK1 Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Monkey WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Death-Associated Protein Kinase 1 (DAPK1) (AA 1141-1430) antibodyDAPK1 Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated
anti-Death-Associated Protein Kinase 1 (DAPK1) (AA 274-323) antibodyDAPK1 Reactivity: Human, Mouse ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated
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- Application Notes
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WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Expiry Date
- 12 months
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: "CpG island hypermethylation of multiple tumor suppressor genes associated with loss of their protein expression during rat lung carcinogenesis induced by 3-methylcholanthrene and diethylnitrosamine." in: Biochemical and biophysical research communications, Vol. 402, Issue 3, pp. 507-14, (2010) (PubMed).
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: "CpG island hypermethylation of multiple tumor suppressor genes associated with loss of their protein expression during rat lung carcinogenesis induced by 3-methylcholanthrene and diethylnitrosamine." in: Biochemical and biophysical research communications, Vol. 402, Issue 3, pp. 507-14, (2010) (PubMed).
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- Target
- DAP Kinase 1 (DAPK1) (Death-Associated Protein Kinase 1 (DAPK1))
- Alternative Name
- DAPK1 (DAPK1 Products)
- Synonyms
- DAPK1 antibody, MGC81366 antibody, si:ch211-66i11.1 antibody, wu:fj09c03 antibody, dapk antibody, MGC83745 antibody, 2310039H24Rik antibody, 2810425C21Rik antibody, AI642003 antibody, D13Ucla1 antibody, DAP-Kinase antibody, DAPK antibody, death-associated protein kinase 1 antibody, death associated protein kinase 1 L homeolog antibody, death associated protein kinase 1 antibody, death associated protein kinase 1 S homeolog antibody, LOC427465 antibody, dapk1.L antibody, DAPK1 antibody, dapk1 antibody, dapk1.S antibody, Dapk1 antibody
- Background
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Synonyms: DAPK, Death-associated protein kinase 1, DAP kinase 1, DAPK1
Background: Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function. Phosphorylates TPM1, enhancing stress fiber formation in endothelial cells. Phosphorylates STX1A and significantly decreases its binding to STXBP1. Phosphorylates PRKD1 and regulates JNK signaling by binding and activating PRKD1 under oxidative stress. Phosphorylates BECN1, reducing its interaction with BCL2 and BCL2L1 and promoting the induction of autophagy. Phosphorylates TSC2, disrupting the TSC1-TSC2 complex and stimulating mTORC1 activity in a growth factor-dependent pathway. Phosphorylates RPS6, MYL9 and DAPK3. Acts as a signaling amplifier of NMDA receptors at extrasynaptic sites for mediating brain damage in stroke. Cerebral ischemia recruits DAPK1 into the NMDA receptor complex and it phosphorylates GRINB at Ser-133 inducing injurious Ca(2+) influx through NMDA receptor channels, resulting in an irreversible neuronal death. Required together with DAPK3 for phosphorylation of RPL13A upon interferon-gamma activation which is causing RPL13A involvement in transcript-selective translation inhibition. Isoform 2 cannot induce apoptosis but can induce membrane blebbing.
- Gene ID
- 1612
- UniProt
- P53355
- Pathways
- MAPK Signaling, Interferon-gamma Pathway
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