ABL1 antibody (pTyr245)
(1 reference)-
- Target See all ABL1 Antibodies
- ABL1 (C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1))
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Binding Specificity
- pTyr245
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Reactivity
- Human, Mouse, Rat, Monkey
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This ABL1 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
- Specificity
- Phospho-c-Abl (Tyr245) Antibody detects endogenous levels of c-Abl only when phosphorylated at Tyrosine 245
- Cross-Reactivity
- Human, Monkey, Mouse (Murine), Rat (Rattus)
- Purification
- The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
- Immunogen
- A synthesized peptide derived from human c-Abl around the phosphorylation site of Tyrosine 245
- Isotype
- IgG
- Top Product
- Discover our top product ABL1 Primary Antibody
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anti-C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1) (AA 490-590) antibody
ABL1 Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1) (pTyr251) antibodyABL1 Reactivity: Human WB, IF, IHC (p), DB Host: Rabbit Polyclonal RB7351 unconjugated
anti-C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1) (pTyr393), (pTyr412) antibodyABL1 Reactivity: Human, Mouse, Monkey WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1) (pTyr412) antibodyABL1 Reactivity: Human, Mouse, Monkey WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1) antibodyABL1 Reactivity: Human, Rat WB, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1) (pTyr412) antibodyABL1 Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated
anti-C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1) (AA 741-769) antibodyABL1 Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1) (pTyr204) antibodyABL1 Reactivity: Human, Mouse, Rat, Monkey WB, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1) (pTyr245) antibodyABL1 Reactivity: Human, Mouse WB, IF, IHC (p) Host: Rabbit Polyclonal RB7349 unconjugated
anti-C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1) (pTyr134) antibodyABL1 Reactivity: Human IHC (p), DB Host: Rabbit Polyclonal RB7343 unconjugated
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- Application Notes
- WB 1:500-1:2000 IF/ICC 1:100-1:500
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20 °C.Stable for 12 months from date of receipt
- Expiry Date
- 12 months
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: "Autophagy as an essential cellular antioxidant pathway in neurodegenerative disease." in: Redox biology, Vol. 2, pp. 82-90, (2015) (PubMed).
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: "Autophagy as an essential cellular antioxidant pathway in neurodegenerative disease." in: Redox biology, Vol. 2, pp. 82-90, (2015) (PubMed).
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- Target
- ABL1 (C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1))
- Alternative Name
- c-Abl (ABL1 Products)
- Synonyms
- ABL antibody, JTK7 antibody, bcr/abl antibody, c-ABL antibody, p150 antibody, v-abl antibody, AI325092 antibody, Abl antibody, E430008G22Rik antibody, c-Abl antibody, ABL proto-oncogene 1, non-receptor tyrosine kinase antibody, c-abl oncogene 1, non-receptor tyrosine kinase antibody, Tyrosine-protein kinase abl-1 antibody, ABL1 antibody, Abl1 antibody, abl-1 antibody, abl1 antibody
- Background
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Description: Non-receptor tyrosine-protein kinase that plays a role in many key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion, receptor endocytosis, autophagy, DNA damage response and apoptosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like WASF3 (involved in branch formation), ANXA1 (involved in membrane anchoring), DBN1, DBNL, CTTN, RAPH1 and ENAH (involved in signaling), or MAPT and PXN (microtubule-binding proteins). Phosphorylation of WASF3 is critical for the stimulation of lamellipodia formation and cell migration. Involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as BCAR1, CRK, CRKL, DOK1, EFS or NEDD9. Phosphorylates multiple receptor tyrosine kinases and more particularly promotes endocytosis of EGFR, facilitates the formation of neuromuscular synapses through MUSK, inhibits PDGFRB-mediated chemotaxis and modulates the endocytosis of activated B-cell receptor complexes. Other substrates which are involved in endocytosis regulation are the caveolin (CAV1) and RIN1. Moreover, ABL1 regulates the CBL family of ubiquitin ligases that drive receptor down-regulation and actin remodeling. Phosphorylation of CBL leads to increased EGFR stability. Involved in late-stage autophagy by regulating positively the trafficking and function of lysosomal components. ABL1 targets to mitochondria in response to oxidative stress and thereby mediates mitochondrial dysfunction and cell death. In response to oxidative stress, phosphorylates serine/threonine kinase PRKD2 at 'Tyr-717' (PubMed:28428613). ABL1 is also translocated in the nucleus where it has DNA-binding activity and is involved in DNA-damage response and apoptosis. Many substrates are known mediators of DNA repair: DDB1, DDB2, ERCC3, ERCC6, RAD9A, RAD51, RAD52 or WRN. Activates the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates TP73, a primary regulator for this type of damage-induced apoptosis. Phosphorylates the caspase CASP9 on 'Tyr-153' and regulates its processing in the apoptotic response to DNA damage. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks. ABL1 acts also as a regulator of multiple pathological signaling cascades during infection. Several known tyrosine-phosphorylated microbial proteins have been identified as ABL1 substrates. This is the case of A36R of Vaccinia virus, Tir (translocated intimin receptor) of pathogenic E.coli and possibly Citrobacter, CagA (cytotoxin-associated gene A) of H.pylori, or AnkA (ankyrin repeat-containing protein A) of A.phagocytophilum. Pathogens can highjack ABL1 kinase signaling to reorganize the host actin cytoskeleton for multiple purposes, like facilitating intracellular movement and host cell exit. Finally, functions as its own regulator through autocatalytic activity as well as through phosphorylation of its inhibitor, ABI1. Regulates T-cell differentiation in a TBX21-dependent manner. Phosphorylates TBX21 on tyrosine residues leading to an enhancement of its transcriptional activator activity (By similarity).
Gene: ABL1
- Molecular Weight
- 135kDa
- Gene ID
- 25
- UniProt
- P00519
- Pathways
- Apoptosis, Regulation of Muscle Cell Differentiation, Platelet-derived growth Factor Receptor Signaling, Lipid Metabolism
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