Immunofluorescence (1:1000) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
In buffer containing 0.09 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,-80 °C
Storage Comment
Store at -20°C or -80°C. Aliquot to avoid repeated freezing and thawing.
Kim, Jeon, Ha, Park, Kim, Shin, Lee, Chung, Lee: "Functional interaction between BubR1 and securin in an anaphase-promoting complex/cyclosomeCdc20-independent manner." in: Cancer research, Vol. 69, Issue 1, pp. 27-36, (2009) (PubMed).
Fujita, Epperly, Zou, Greenberger, Wan: "Regulation of the anaphase-promoting complex-separase cascade by transforming growth factor-beta modulates mitotic progression in bone marrow stromal cells." in: Molecular biology of the cell, Vol. 19, Issue 12, pp. 5446-55, (2008) (PubMed).
Rubinek, Chesnokova, Wolf, Wawrowsky, Vlotides, Melmed: "Discordant proliferation and differentiation in pituitary tumor-transforming gene-null bone marrow stem cells." in: American journal of physiology. Cell physiology, Vol. 293, Issue 3, pp. C1082-92, (2007) (PubMed).
Chestukhin, Pfeffer, Milligan, DeCaprio, Pellman: "Processing, localization, and requirement of human separase for normal anaphase progression." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 8, pp. 4574-9, (2003) (PubMed).
Target
Separase (ESPL1)
(Extra Spindle Poles Like 1 (ESPL1))