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AIRE antibody (C-Term)

Name: AIRE antibody (C-Term)
Brand: antibodies-online
SKU: ABIN184780
Price: 469.28 USD
Availability: InStock

Verified AIRE Reactivity: Human WB, ELISA, IF, IHC, FACS Host: Goat Polyclonal unconjugated

ABIN184780 (1µg/ml) staining of nuclear Jurkat cell (A), (2ug/ml) Human Spleen (B) and negative control Human Smooth Muscle (C) lysate (35µg protein in RIPA buffer).

IHC

ABIN184780 (2µg/ml) staining of paraffin embedded Human Thymus.

IHC

ABIN184780 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.

ABIN184780 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.

ABIN184780 Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.

FACS

8 images

Catalog No. ABIN184780

Datasheet as PDF

Target See all AIRE Antibodies

AIRE (Autoimmune Regulator (AIRE))
Binding Specificity
10

9

5

5

3

3

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
C-Term
Reactivity
51

23

7

5

3

3

3

3

2
Human
Host
47

6

1
Goat
Compatible Secondaries
Clonality
53
Polyclonal
Conjugate
38

3

3

2

1

1

1

1

1

1

1

1
This AIRE antibody is un-conjugated
Application
39

24

18

7

7

2

1

1

1

1

1

1
Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Purpose

AIRE

Sequence

QSMARPAAPF PS

Cross-Reactivity

Human

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade

Verified

Immunogen

Peptide with sequence C-QSMARPAAPFPS, from the C Terminus of the protein sequence according to NP_000374.1, NP_000649.1.

Isotype

IgG
anti-Autoimmune Regulator (AIRE) (AA 30-130) antibody
AIRE Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated

anti-Autoimmune Regulator (AIRE) (N-Term) antibody
AIRE Reactivity: Human, Mouse, Rat, Dog, Horse, Cow, Guinea Pig WB, IHC Host: Rabbit Polyclonal unconjugated

anti-Autoimmune Regulator (AIRE) (Internal Region) antibody
AIRE Reactivity: Human, Mouse, Rat WB, ELISA, IF, IHC, ICC Host: Rabbit Polyclonal unconjugated

anti-Autoimmune Regulator (AIRE) (Internal Region) antibody
Verified AIRE Reactivity: Human, Pig WB, ELISA, IHC Host: Goat Polyclonal unconjugated

anti-Autoimmune Regulator (AIRE) (AA 39-108) antibody
AIRE Reactivity: Human ELISA, IF, IHC Host: Rabbit Polyclonal unconjugated

anti-Autoimmune Regulator (AIRE) (AA 64-92) antibody
AIRE Reactivity: Human WB, IF Host: Rabbit Polyclonal RB30050 unconjugated

anti-Autoimmune Regulator (AIRE) (Internal Region) antibody
AIRE Reactivity: Human, Mouse WB, ELISA, IF Host: Rabbit Polyclonal unconjugated

anti-Autoimmune Regulator (AIRE) (pSer156) antibody
AIRE Reactivity: Human WB, ELISA Host: Rabbit Polyclonal unconjugated

anti-Autoimmune Regulator (AIRE) (Internal Region) antibody
Verified AIRE Reactivity: Human, Mouse WB, ELISA Host: Goat Polyclonal unconjugated

anti-Autoimmune Regulator (AIRE) antibody
AIRE Reactivity: Human, Mouse IF Host: Rabbit Polyclonal unconjugated

Application Notes

Immunohistochemistry: In paraffin embedded Human Thymic medula shows staining of select nuclei. Recommended concentration: 2.5 μg/mL.
Western Blot: Preliminary testing showed an approx 60 kDa band in nuclear lysates of cell line Jurkat and in Human Spleen lysates (calculated MW of 57.7 kDa according to NP_000374.1). Recommended concentration: 1-2 μg/mL. Primary incubation 1 hour at room t
Peptide ELISA: antibody detection limit dilution 1:128000.

Comment

Immunofluorescence: Strong expression of the protein seen in the nucleoplasm and nuclear membranes of U2OS and HeLa cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of Jurkat cells. R

Restrictions

For Research Use only
Format

Liquid

Concentration

0.5 mg/mL

Buffer

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Minimize freezing and thawing.

Storage

-20 °C

Storage Comment

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Target

AIRE (Autoimmune Regulator (AIRE))

Alternative Name

AIRE (AIRE Products)

Synonyms

AIRE1 antibody, APECED antibody, APS1 antibody, APSI antibody, PGA1 antibody, autoimmune regulator antibody, autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy) antibody, AaeL_AAEL013794 antibody, CpipJ_CPIJ001208 antibody, CpipJ_CPIJ001209 antibody, AIRE antibody, Aire antibody

Background

AIRE, autoimmune regulator (automimmune polyendocrinopathy candidiasis ectodermal dystrophy), APS1, APSI, PGA1, APECED, AIRE protein, autoimmune regulator (APECED protein), autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrop

Gene ID

326

NCBI Accession

NP_000374

Pathways

Chromatin Binding