DNA2 antibody (Internal Region)
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- Target See all DNA2 Antibodies
- DNA2 (DNA Replication Helicase 2 Homolog (DNA2))
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Binding Specificity
- Internal Region
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Reactivity
- Human, Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This DNA2 antibody is un-conjugated
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Application
- ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
- Specificity
- DNA2L Antibody detects endogenous levels of total DNA2L.
- Predicted Reactivity
- Pig,Bovine,Horse,Sheep,Rabbit,Dog
- Purification
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- Immunogen
- A synthesized peptide derived from human DNA2L, corresponding to a region within the internal amino acids.
- Isotype
- IgG
- Top Product
- Discover our top product DNA2 Primary Antibody
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anti-DNA Replication Helicase 2 Homolog (DNA2) (AA 1-300) antibody
DNA2 Reactivity: Human ELISA, IHC Host: Rabbit Polyclonal unconjugated
anti-DNA Replication Helicase 2 Homolog (DNA2) antibodyDNA2 Reactivity: Human ELISA, IHC Host: Rabbit Polyclonal unconjugated
anti-DNA Replication Helicase 2 Homolog (DNA2) (Middle Region) antibodyDNA2 Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit WB Host: Rabbit Polyclonal unconjugated
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- Application Notes
- WB 1:1000-3000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20 °C. Stable for 12 months from date of receipt.
- Expiry Date
- 12 months
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- Target
- DNA2 (DNA Replication Helicase 2 Homolog (DNA2))
- Alternative Name
- DNA2 (DNA2 Products)
- Synonyms
- DNA2L antibody, Dna2l antibody, E130315B21Rik antibody, PEOA6 antibody, hDNA2 antibody, XDna2 antibody, dna2-A antibody, DNA replication helicase/nuclease 2 antibody, DNA replication helicase/nuclease 2 S homeolog antibody, DNA2 antibody, Dna2 antibody, dna2.S antibody
- Background
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Description: Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break (DSB) repair: recruited by BLM and mediates the cleavage of 5'-ssDNA, while the 3'-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing. Possesses different enzymatic activities, such as single-stranded DNA (ssDNA)-dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is subject to debate. According to various reports, the helicase activity is weak and its function remains largely unclear. Helicase activity may promote the motion of DNA2 on the flap, helping the nuclease function.
Gene: DNA2
- Molecular Weight
- 120 kDa
- Gene ID
- 1763
- UniProt
- P51530
- Pathways
- Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA
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