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ICAM-3/CD50 antibody

Name: ICAM-3/CD50 antibody
Brand: antibodies-online
SKU: ABIN94167
Price: 333.96 USD
Availability: InStock

ICAM3 Reactivity: Human FACS, IP Host: Mouse Monoclonal MEM-171 unconjugated

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD50 (MEM-171) purified antibody (concentration in sample 0,6 μg/mL, GAM APC).

FACS

Surface staining of JURKAT humanleukemia T cell line with anti-human CD50 (MEM-171) FITC.

FACS

Separation of human lymphocytes (red-filled) from CD50 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD50 (MEM-171) purified antibody (concentration in sample 0,6 μg/mL, GAM APC).

FACS

3 images

(2 references)

Catalog No. ABIN94167

Datasheet as PDF

Target See all ICAM-3/CD50 (ICAM3) Antibodies

ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))
Reactivity
111

3

2

2
Human
Host
67

43

1
Mouse
Compatible Secondaries
Clonality
71

40
Monoclonal
Conjugate
60

10

6

6

3

3

3

3

3

3

3

2

1

1

1

1

1

1
This ICAM-3/CD50 antibody is un-conjugated
Application
61

49

47

34

26

25

22

12

7

7

7

6

6

4

4

2

1

1

1

1

1

1
Flow Cytometry (FACS), Immunoprecipitation (IP)

Specificity

The antibody MEM-171 recognizes an extracellular epitope in the D2 domain of CD50 (ICAM-3), a 120-130 kDa type I membrane protein (immunoglobulin supergene family) expressed on leukocytes, endothelial cells and Langerhans cells, it is negative on platelets and erythrocytes.

Cross-Reactivity (Details)

Human

Purification

Purified by protein-A affinity chromatography.

Purity

> 95 % (by SDS-PAGE)

Immunogen

Human granulocytes

Clone

MEM-171

Isotype

IgG1
anti-Intercellular Adhesion Molecule 3 (ICAM3) antibody
ICAM3 Reactivity: Human FACS, IHC (p), Func Host: Mouse Monoclonal MEM-04 unconjugated

anti-Intercellular Adhesion Molecule 3 (ICAM3) (AA 30-203) antibody
ICAM3 Reactivity: Human FACS, WB, ELISA, IHC, ICC, Neut Host: Mouse Monoclonal 2H10G7 unconjugated

anti-Intercellular Adhesion Molecule 3 (ICAM3) (AA 200-547) antibody
ICAM3 Reactivity: Human WB, IF, ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-Intercellular Adhesion Molecule 3 (ICAM3) antibody (Biotin)
ICAM3 Reactivity: Human FACS, IP Host: Mouse Monoclonal MEM-171 Biotin

anti-Intercellular Adhesion Molecule 3 (ICAM3) antibody (PE)
ICAM3 Reactivity: Human FACS Host: Mouse Monoclonal MEM-171 PE

anti-Intercellular Adhesion Molecule 3 (ICAM3) (AA 30-547) antibody
ICAM3 Reactivity: Human WB, ELISA, IHC (p) Host: Mouse Monoclonal 2F8 unconjugated

anti-Intercellular Adhesion Molecule 3 (ICAM3) (AA 46-197) antibody
ICAM3 Reactivity: Human WB, IHC, ICC Host: Rabbit Polyclonal unconjugated

anti-Intercellular Adhesion Molecule 3 (ICAM3) (AA 259-491) antibody
ICAM3 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Intercellular Adhesion Molecule 3 (ICAM3) (AA 30-130) antibody
ICAM3 Reactivity: Human ELISA, IHC (p), ICC, IHC (fro), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

anti-Intercellular Adhesion Molecule 3 (ICAM3) (Internal Region) antibody
ICAM3 Reactivity: Human, Mouse WB, IF, ELISA, IHC, ICC Host: Rabbit Polyclonal unconjugated

Application Notes

Flow cytometry: Recommended dilution: 4 μg/mL.

Restrictions

For Research Use only
Concentration

1 mg/mL

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Do not freeze.

Storage

4 °C

Storage Comment

Store at 2-8°C. Do not freeze.
Linnebacher, Wienck, Boeck, Klar: "Identification of an MSI-H tumor-specific cytotoxic T cell epitope generated by the (-1) frame of U79260(FTO)." in: Journal of biomedicine & biotechnology, Vol. 2010, pp. 841451, (2010) (PubMed).

Cermák, Símová, Pintzas, Horejsí, Andera: "Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules." in: The Journal of biological chemistry, Vol. 277, Issue 10, pp. 7955-61, (2002) (PubMed).
Target

ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

Alternative Name

CD50 (ICAM3 Products)

Synonyms

CD50 antibody, CDW50 antibody, ICAM-R antibody, ICAM-3 antibody, ICAM3 antibody, intercellular adhesion molecule 3 antibody, ICAM3 antibody, Icam3 antibody

Background

Intercellular adhesion molecule 3,CD50 (intracellular adhesion molecule 3, ICAM-3) is a transmembrane glycoprotein expressed by leukocytes, that serves as a counter-receptor for the lymphocyte function-associated antigen (LFA)-1 integrin. Besides functioning as an adhesive molecule that mediates e.g. the contact between T cells and antigen presenting cells, ICAM-3 regulates affinity of LFA-1 for ICAM-1 and induces T cell activation and proliferation. ICAM-3 plays an essential role in the initiation of the immune response both on T cells and antigen presenting cells and interacts also with CD209 (dendritic cell-specific ICAM-3-grabbing nonintegrin, DC-SIGN), a C-type lectin of dendritic cells and macrophages, this process is involved in dialogue between dendritic cells and granulocytes.,ICAM-3, ICAM-R

Gene ID

3385

UniProt

P32942