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IL5RA antibody (AA 21-196)

Name: IL5RA antibody (AA 21-196)
Brand: antibodies-online
SKU: ABIN7193317
Price: 511.5 USD
Availability: InStock

IL5RA Reactivity: Human WB, ELISA, IHC, FACS, ICC, Neut Host: Mouse Monoclonal 5E12F5D6 unconjugated

Western blot analysis using CD125 mAb against human CD125 (AA: extra 21-196) recombinant protein.

Flow cytometric analysis of HL-60 cells using CD125 mouse mAb (green) and negative control (red).

FACS

Black line: Control Antigen (100 ng),Purple line: Antigen (10 ng), Blue line: Antigen (50 ng), Red line:Antigen (100 ng)

ELISA

Western blot analysis using CD125 mAb against HEK293 (1) and CD125 (AA: extra 21-196)-hIgGFc transfected HEK293 (2) cell lysate.

4 images

Catalog No. ABIN7193317

Datasheet as PDF

Target See all IL5RA Antibodies

IL5RA (Interleukin 5 Receptor, alpha (IL5RA))
Binding Specificity
15

14

11

8

7

6

2

2

1

1

1

1

1

1

1

1
AA 21-196
Reactivity
72

31

8

6

2

2

2

2

2

1
Human
Host
69

4
Mouse
Compatible Secondaries
Clonality
70

2

1
Monoclonal
Conjugate
31

8

5

5

5

4

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This IL5RA antibody is un-conjugated
Application
68

36

25

15

13

13

6

3

3

2

2

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Neutralization (Neut)

Purification

purified

Immunogen

Purified recombinant fragment of human CD125 (AA: extra 21-196) expressed in E. coli.

Clone

5E12F5D6

Isotype

IgG1

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Application Notes

ELISA: 1:10000, WB: 1:500 - 1:2000, FCM: 1:200 - 1:400, ICC: N/A, IHC: N/A

Restrictions

For Research Use only
Buffer

Purified antibody in PBS with 0.05 % sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C/-20 °C

Storage Comment

4°C, -20°C for long term storage
Target

IL5RA (Interleukin 5 Receptor, alpha (IL5RA))

Alternative Name

CD125 (IL5RA Products)

Synonyms

MGC86517 antibody, IL5RA antibody, il5r antibody, cdw125 antibody, hsil5r3 antibody, gpIL-5ra antibody, CD125 antibody, CDw125 antibody, HSIL5R3 antibody, IL5R antibody, Il5r antibody, interleukin 5 receptor subunit alpha L homeolog antibody, interleukin 5 receptor subunit alpha antibody, interleukin 5 receptor, alpha antibody, il5ra.L antibody, IL5RA antibody, il5ra antibody, Il5ra antibody

Background

Description: The protein encoded by this gene is an interleukin 5 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL5 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4. Several alternatively spliced transcript variants encoding four distinct isoforms have been reported.
Aliases: IL5RA, IL5R, CDw125, HSIL5R3

Molecular Weight

47.7 kDa

Gene ID

3568

Pathways

JAK-STAT Signaling