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ILK antibody (AA 97-244)

Name: ILK antibody (AA 97-244)
Brand: antibodies-online
SKU: ABIN1098122
Price: 511.5 USD
Availability: InStock

ILK Reactivity: Human, Mouse, Monkey WB, ELISA, IHC, FACS Host: Mouse Monoclonal 3A9 unconjugated

Western blot analysis using ILK mouse mAb against Jurkat (1), NIH3T3 (2), HeLa (3), PC-12 (4), C6 (5), COS7 (6), Raji (7), K562 (8) and MCF-7 (9) cell lysate.

Flow cytometric analysis of Jurkat cells using ILK mouse mAb (green) and negative control (red).

FACS

Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using ILK mouse mAb with DAB staining.

IHC

Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ILK mouse mAb with DAB staining.

IHC

Western blot analysis using ILK mAb against human ILK recombinant protein.

Black line: Control Antigen (100 ng), Purple line: Antigen(10 ng), Blue line: Antigen (50 ng), Red line: Antigen (100 ng),

ELISA

6 images

(2 references)

Catalog No. ABIN1098122

Datasheet as PDF

Target See all ILK Antibodies

ILK (Integrin-Linked Kinase (ILK))
Binding Specificity
25

24

16

15

10

9

7

7

6

6

6

4

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1
AA 97-244
Reactivity
148

58

52

3

1

1

1

1

1

1

1
Human, Mouse, Monkey
Host
158

4

1
Mouse
Compatible Secondaries
Clonality
157

6
Monoclonal
Conjugate
62

14

14

12

8

8

5

5

5

5

5

5

3

3

3

3

3
This ILK antibody is un-conjugated
Application
111

86

54

40

39

20

17

9

6

5

5

3

3

2

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Purification

purified

Immunogen

Purified recombinant fragment of human ILK (AA: 97-244) expressed in E. coli.

Clone

3A9

Isotype

IgG1
anti-Integrin-Linked Kinase (ILK) antibody
KO Validated ILK Reactivity: Human WB, IHC, IHC (p), IP, IHC (wm) Host: Rabbit Polyclonal unconjugated

anti-Integrin-Linked Kinase (ILK) (AA 341-452) antibody
ILK Reactivity: Human WB, ELISA, IF, RNAi Host: Mouse Monoclonal 4F10 unconjugated

anti-Integrin-Linked Kinase (ILK) (AA 301-400) antibody
ILK Reactivity: Human, Mouse, Rat WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Integrin-Linked Kinase (ILK) (AA 1-260) antibody
ILK Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated

anti-Integrin-Linked Kinase (ILK) (AA 11-41), (N-Term) antibody
ILK Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB1668 unconjugated

anti-Integrin-Linked Kinase (ILK) (AA 225-253) antibody
ILK Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB13868 unconjugated

anti-Integrin-Linked Kinase (ILK) (AA 225-253) antibody
ILK Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-Integrin-Linked Kinase (ILK) (AA 11-41) antibody
ILK Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-Integrin-Linked Kinase (ILK) (AA 1-228) antibody
ILK Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Integrin-Linked Kinase (ILK) (pThr173) antibody
ILK Reactivity: Human, Rat WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Application Notes

ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, FCM: 1:200 - 1:400

Restrictions

For Research Use only
Format

Liquid

Buffer

Ascitic fluid containing 0.03 % sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C/-20 °C

Storage Comment

4°C, -20°C for long term storage
Taylor, Qiao, Colon, Schlegel, Josifi, Chung: "Integrin-linked kinase regulates phosphatase and tensin homologue activity to promote tumorigenesis in neuroblastoma cells." in: Surgery, Vol. 150, Issue 2, pp. 162-8, (2011) (PubMed).

Matsui, Assi, Ogawa, Raven, Dedhar, Gleave, Salh, So: "The importance of integrin-linked kinase in the regulation of bladder cancer invasion." in: International journal of cancer. Journal international du cancer, Vol. 130, Issue 3, pp. 521-31, (2011) (PubMed).
Target

ILK (Integrin-Linked Kinase (ILK))

Alternative Name

ILK (ILK Products)

Synonyms

CG10504 antibody, CT29478 antibody, DmILK antibody, Dmel\\CG10504 antibody, ILK antibody, ilk antibody, l(3)78Ca antibody, p59 antibody, AA511515 antibody, ESTM24 antibody, zgc:65842 antibody, ILK-1 antibody, ILK-2 antibody, P59 antibody, p59ILK antibody, Integrin linked kinase antibody, integrin linked kinase antibody, integrin linked kinase S homeolog antibody, integrin-linked protein kinase antibody, integrin-linked kinase antibody, Ilk antibody, ILK antibody, ilk.S antibody, CpipJ_CPIJ016644 antibody, Bm1_20815 antibody, Tsp_07213 antibody, Tsp_07224 antibody, Tsp_15354 antibody, ilk antibody

Background

Description: Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.
Aliases: P59, ILK-2

Molecular Weight

51.4 kDa

Gene ID

3611

HGNC

3611

Pathways

Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Maintenance of Protein Location, Skeletal Muscle Fiber Development, Smooth Muscle Cell Migration