ALKBH3 antibody (Internal Region)
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- Target See all ALKBH3 Antibodies
- ALKBH3 (AlkB, Alkylation Repair Homolog 3 (ALKBH3))
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Binding Specificity
- Internal Region
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This ALKBH3 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Specificity
- ALKBH3 Antibody detects endogenous levels of total ALKBH3.
- Predicted Reactivity
- Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus
- Purification
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- Immunogen
- A synthesized peptide derived from human ALKBH3, corresponding to a region within the internal amino acids.
- Isotype
- IgG
- Top Product
- Discover our top product ALKBH3 Primary Antibody
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anti-AlkB, Alkylation Repair Homolog 3 (ALKBH3) (AA 1-170) antibody
ALKBH3 Reactivity: Human ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-AlkB, Alkylation Repair Homolog 3 (ALKBH3) (AA 1-139) antibodyALKBH3 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 2A5-4F5 unconjugated
anti-AlkB, Alkylation Repair Homolog 3 (ALKBH3) (AA 242-269), (C-Term) antibodyALKBH3 Reactivity: Human, Mouse WB, IHC (p) Host: Rabbit Polyclonal RB22074 unconjugated
anti-AlkB, Alkylation Repair Homolog 3 (ALKBH3) (AA 1-286) antibodyALKBH3 Reactivity: Human WB, IP Host: Rabbit Polyclonal unconjugated
anti-AlkB, Alkylation Repair Homolog 3 (ALKBH3) (AA 216-265) antibodyALKBH3 Reactivity: Human, Mouse, Rat, Horse, Rabbit, Bat, Monkey WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-AlkB, Alkylation Repair Homolog 3 (ALKBH3) (AA 1-286) antibodyALKBH3 Reactivity: Human WB Host: Rabbit Polyclonal unconjugated
anti-AlkB, Alkylation Repair Homolog 3 (ALKBH3) (Middle Region) antibodyALKBH3 Reactivity: Human, Mouse, Rat, Horse, Rabbit, Cow, Dog, Guinea Pig, Pig WB Host: Rabbit Polyclonal unconjugated
anti-AlkB, Alkylation Repair Homolog 3 (ALKBH3) (AA 1-286) antibody (APC)ALKBH3 Reactivity: Human IHC, IF, FACS, ICC Host: Rabbit Polyclonal APC
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- Application Notes
- WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Expiry Date
- 12 months
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- Target
- ALKBH3 (AlkB, Alkylation Repair Homolog 3 (ALKBH3))
- Alternative Name
- ALKBH3 (ALKBH3 Products)
- Synonyms
- ABH3 antibody, DEPC-1 antibody, DEPC1 antibody, PCA1 antibody, 1700108H04Rik antibody, 1810020C19Rik antibody, Abh3 antibody, mABH3 antibody, alkB homolog 3, alpha-ketoglutarate-dependent dioxygenase L homeolog antibody, alkB homolog 3, alpha-ketoglutaratedependent dioxygenase antibody, alkB homolog 3, alpha-ketoglutarate-dependent dioxygenase antibody, alkbh3.L antibody, ALKBH3 antibody, Alkbh3 antibody
- Background
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Description: Dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A) (PubMed:12486230, PubMed:12594517, PubMed:16174769, PubMed:26863196, PubMed:26863410). Repairs alkylated DNA containing 1-methyladenosine (m1A) and 3-methylcytosine (m3C) by oxidative demethylation (PubMed:12486230, PubMed:12594517, PubMed:16174769, PubMed:25944111). Has a strong preference for single-stranded DNA (PubMed:12486230, PubMed:12594517, PubMed:16174769). Able to process alkylated m3C within double-stranded regions via its interaction with ASCC3, which promotes DNA unwinding to generate single-stranded substrate needed for ALKBH3 (PubMed:22055184). Also acts on RNA (PubMed:12594517, PubMed:16174769, PubMed:26863196, PubMed:26863410, PubMed:16858410). Demethylates N1-methyladenosine (m1A) RNA, an epigenetic internal modification of messenger RNAs (mRNAs) highly enriched within 5'-untranslated regions (UTRs) and in the vicinity of start codons (PubMed:26863196, PubMed:26863410). Requires molecular oxygen, alpha-ketoglutarate and iron (PubMed:22055184, PubMed:16858410).
Gene: ALKBH3
- Molecular Weight
- 33 kDa
- Gene ID
- 221120
- UniProt
- Q96Q83
- Pathways
- DNA Damage Repair
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