BTK antibody (Internal Region)
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- Target See all BTK Antibodies
- BTK (Bruton Agammaglobulinemia tyrosine Kinase (BTK))
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Binding Specificity
- Internal Region
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This BTK antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
- Specificity
- BTK Antibody detects endogenous levels of total BTK.
- Predicted Reactivity
- Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus
- Purification
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- Immunogen
- A synthesized peptide derived from human BTK, corresponding to a region within the internal amino acids.
- Isotype
- IgG
- Top Product
- Discover our top product BTK Primary Antibody
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anti-Bruton Agammaglobulinemia tyrosine Kinase (BTK) (AA 30-240) antibody
BTK Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated
anti-Bruton Agammaglobulinemia tyrosine Kinase (BTK) (AA 209-239) antibodyBTK Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Bruton Agammaglobulinemia tyrosine Kinase (BTK) (AA 27-55) antibodyBTK Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated
anti-Bruton Agammaglobulinemia tyrosine Kinase (BTK) (AA 254-443) antibodyBTK Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Bruton Agammaglobulinemia tyrosine Kinase (BTK) (pSer180) antibodyBTK Reactivity: Mouse, Rat WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Bruton Agammaglobulinemia tyrosine Kinase (BTK) (AA 209-239) antibodyBTK Reactivity: Human WB, IF, IHC (p) Host: Rabbit Polyclonal RB03117 unconjugated
anti-Bruton Agammaglobulinemia tyrosine Kinase (BTK) (AA 27-55), (N-Term) antibodyBTK Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB33691 unconjugated
anti-Bruton Agammaglobulinemia tyrosine Kinase (BTK) antibodyBTK Reactivity: Human, Monkey WB, ELISA, IHC, ICC Host: Mouse Monoclonal 7F12H4 unconjugated
anti-Bruton Agammaglobulinemia tyrosine Kinase (BTK) (C-Term) antibodyBTK Reactivity: Human, Mouse WB, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Bruton Agammaglobulinemia tyrosine Kinase (BTK) (AA 91-387) antibodyBTK Reactivity: Human WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
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- Application Notes
- WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20 °C. Stable for 12 months from date of receipt.
- Expiry Date
- 12 months
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- Target
- BTK (Bruton Agammaglobulinemia tyrosine Kinase (BTK))
- Alternative Name
- BTK (BTK Products)
- Synonyms
- BTK antibody, atk antibody, bpk antibody, xla antibody, imd1 antibody, agmx1 antibody, psctk1 antibody, AGMX1 antibody, AT antibody, ATK antibody, BPK antibody, IMD1 antibody, PSCTK1 antibody, XLA antibody, AI528679 antibody, xid antibody, Bruton tyrosine kinase antibody, Bruton agammaglobulinemia tyrosine kinase antibody, BTK antibody, btk antibody, Btk antibody
- Background
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Description: Non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. After BCR engagement and activation at the plasma membrane, phosphorylates PLCG2 at several sites, igniting the downstream signaling pathway through calcium mobilization, followed by activation of the protein kinase C (PKC) family members. PLCG2 phosphorylation is performed in close cooperation with the adapter protein B-cell linker protein BLNK. BTK acts as a platform to bring together a diverse array of signaling proteins and is implicated in cytokine receptor signaling pathways. Plays an important role in the function of immune cells of innate as well as adaptive immunity, as a component of the Toll-like receptors (TLR) pathway. The TLR pathway acts as a primary surveillance system for the detection of pathogens and are crucial to the activation of host defense. Especially, is a critical molecule in regulating TLR9 activation in splenic B-cells. Within the TLR pathway, induces tyrosine phosphorylation of TIRAP which leads to TIRAP degradation. BTK plays also a critical role in transcription regulation. Induces the activity of NF-kappa-B, which is involved in regulating the expression of hundreds of genes. BTK is involved on the signaling pathway linking TLR8 and TLR9 to NF-kappa-B. Transiently phosphorylates transcription factor GTF2I on tyrosine residues in response to BCR. GTF2I then translocates to the nucleus to bind regulatory enhancer elements to modulate gene expression. ARID3A and NFAT are other transcriptional target of BTK. BTK is required for the formation of functional ARID3A DNA-binding complexes. There is however no evidence that BTK itself binds directly to DNA. BTK has a dual role in the regulation of apoptosis.
Gene: BTK
- Molecular Weight
- 76kDa
- Gene ID
- 695
- UniProt
- Q06187
- Pathways
- Fc-epsilon Receptor Signaling Pathway, Hormone Transport, Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, BCR Signaling
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