KLKB1 Protein (AA 20-638) (His tag)
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- Target See all KLKB1 Proteins
- KLKB1 (Kallikrein B, Plasma (Fletcher Factor) 1 (KLKB1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 20-638
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This KLKB1 protein is labelled with His tag.
- Purpose
- Human KLKB1 Protein
- Sequence
- Gly20-Ala638
- Characteristics
- Recombinant Human KLKB1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly20-Ala638.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human KLKB1, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-KLKB1 Antibody, hFc Tag with the EC50 of 21.4ng/ml determined by ELISA. See testing image for detail.
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Kallikrein B, Plasma (Fletcher Factor) 1 (KLKB1) (AA 20-638) protein (His tag)
KLKB1 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Kallikrein B, Plasma (Fletcher Factor) 1 (KLKB1) (AA 20-638) protein (His tag)KLKB1 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Kallikrein B, Plasma (Fletcher Factor) 1 (KLKB1) (AA 20-390) protein (His tag)Crystallography grade KLKB1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
Kallikrein B, Plasma (Fletcher Factor) 1 (KLKB1) (AA 391-638) protein (His tag)Crystallography grade KLKB1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
Kallikrein B, Plasma (Fletcher Factor) 1 (KLKB1) (AA 20-390) protein (His tag)Crystallography grade KLKB1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
Kallikrein B, Plasma (Fletcher Factor) 1 (KLKB1) (AA 391-638) protein (His tag)Crystallography grade KLKB1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
Kallikrein B, Plasma (Fletcher Factor) 1 (KLKB1) (AA 391-638) protein (His tag)KLKB1 Origin: Rat Host: Yeast Recombinant > 90 % ELISA
Kallikrein B, Plasma (Fletcher Factor) 1 (KLKB1) (AA 391-638), (Light Chain) protein (His tag)KLKB1 Origin: Rat Host: Yeast Recombinant > 90 % SDS
Kallikrein B, Plasma (Fletcher Factor) 1 (KLKB1) (AA 391-638), (Light Chain) protein (His tag)KLKB1 Origin: Rat Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Kallikrein B, Plasma (Fletcher Factor) 1 (KLKB1) protein (Myc-DYKDDDDK Tag)KLKB1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in 20 mM NaAc,150 mM NaCl ( pH 5.0).
- Buffer
- Lyophilized from 0.22μm filtered solution in 20 mM NaAc,150 mM NaCl ( pH 5.0). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- KLKB1 (Kallikrein B, Plasma (Fletcher Factor) 1 (KLKB1))
- Alternative Name
- KLKB1 (KLKB1 Products)
- Synonyms
- KLK3 Protein, PPK Protein, APS Protein, Kal-3 Protein, Kal3 Protein, Klk3 Protein, PSA Protein, KALP15 Protein, Kalp1 Protein, KLKB1 Protein, DKFZp459J2015 Protein, klkb1 Protein, kallikrein B1 Protein, kallikrein B, plasma 1 Protein, plasma kallikrein Protein, Plasma kallikrein Protein, kallikrein B, plasma (Fletcher factor) 1 Protein, coagulation factor XI Protein, kallikrein B1 L homeolog Protein, KLKB1 Protein, Klkb1 Protein, CpipJ_CPIJ004042 Protein, CpipJ_CPIJ014955 Protein, CpipJ_CPIJ020108 Protein, VAA_02266 Protein, LOC100549617 Protein, klkb1 Protein, klkb1.L Protein
- Background
- Plasma kallikrein, also known as Fletcher factor or kallikrein B1 (KLKB1), is a serine endopeptidase, like its homologs tissue kallikrein and kallikrein-related peptidases (KLKs). Its physiological role is to catalyze the release of kinins and other vasoactive peptides.
- Molecular Weight
- 70.3 kDa. Due to glycosylation, the protein migrates to 72-80 kDa based on Tris-Bis PAGE result.
- UniProt
- P03952
- Pathways
- Complement System, Brown Fat Cell Differentiation
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