LPAR5 antibody (Cytoplasmic Domain)
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- Target See all LPAR5 Antibodies
- LPAR5 (Lysophosphatidic Acid Receptor 5 (LPAR5))
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Binding Specificity
- Cytoplasmic Domain
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Reactivity
- Human, Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This LPAR5 antibody is un-conjugated
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Application
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Human LPAR5 / GPR92. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Predicted Reactivity
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Mouse, Panda (100%) Marmoset, Elephant, Bat, Bovine (95%) Rat, Dog, Rabbit, Pig, Opossum (89%) Monkey (84%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic 19 amino acid peptide from 3rd cytoplasmic domain of human LPAR5 / GPR92. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Mouse, Panda (100%), Marmoset, Elephant, Bat, Bovine (95%), Rat, Dog, Rabbit, Pig, Opossum (89%), Monkey (84%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product LPAR5 Primary Antibody
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anti-Lysophosphatidic Acid Receptor 5 (LPAR5) (AA 310-326) antibody
LPAR5 Reactivity: Human ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Lysophosphatidic Acid Receptor 5 (LPAR5) (Internal Region) antibodyLPAR5 Reactivity: Human WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Lysophosphatidic Acid Receptor 5 (LPAR5) (Extracellular Domain) antibodyLPAR5 Reactivity: Human ELISA, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Lysophosphatidic Acid Receptor 5 (LPAR5) (Extracellular Domain) antibodyLPAR5 Reactivity: Human, Monkey IHC, IHC (p), ICC Host: Rabbit Polyclonal unconjugated
anti-Lysophosphatidic Acid Receptor 5 (LPAR5) (Internal Region) antibodyLPAR5 Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Lysophosphatidic Acid Receptor 5 (LPAR5) (2nd Extracellular Domain) antibodyLPAR5 Reactivity: Human, Monkey, Gorilla IHC (p), ICC Host: Rabbit Polyclonal unconjugated
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- Application Notes
- Approved: IHC, IHC-P (1 - 5 μg/mL)
- Comment
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Target Species of Antibody: Human
- Assay Procedure
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, less than 0.1 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- LPAR5 (Lysophosphatidic Acid Receptor 5 (LPAR5))
- Alternative Name
- LPAR5 / GPR92 (LPAR5 Products)
- Synonyms
- LPA5 antibody, GPR92 antibody, GPR93 antibody, KPG_010 antibody, LPAR5 antibody, Gpr92 antibody, RGD1562580 antibody, Gm1072 antibody, zgc:194481 antibody, zgc:194493 antibody, lpar6l antibody, p2ry5 antibody, wu:fb62d01 antibody, zgc:66034 antibody, lysophosphatidic acid receptor 5 antibody, lysophosphatidic acid receptor 5b antibody, lysophosphatidic acid receptor 5a antibody, LPAR5 antibody, Lpar5 antibody, lpar5 antibody, lpar5b antibody, lpar5a antibody
- Background
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Name/Gene ID: LPAR5
Subfamily: Orphan-A
Family: GPCR
Synonyms: LPAR5, G-protein coupled receptor 92, GPR92, GPR93, LPA receptor 5, LPA-5, KPG_010, LPA5, G protein-coupled receptor 92, G-protein coupled receptor 93 - Gene ID
- 57121
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