Cytokeratin 18 antibody
(4 references)-
- Target See all Cytokeratin 18 (KRT18) Antibodies
- Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Cytokeratin 18 antibody is un-conjugated
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Application
- Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Specificity
- The mouse monoclonal antibody DC-10 reacts with cytokeratin 18 (45 kDa). Cytokeratins are members of intermediate filaments subfamily of intracellular proteins, represented in epithelial tissues.
- No Cross-Reactivity
- Cow, Dog, Hamster, Mouse, Pig, Rat, Sheep
- Cross-Reactivity (Details)
- Human
- Purification
- Purified by protein-A affinity chromatography.
- Purity
- > 95 % (by SDS-PAGE)
- Immunogen
- Human breast carcinoma cell line PMC-42.
- Clone
- DC-10
- Isotype
- IgG1
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anti-Keratin 18 (KRT18) antibody
KRT18 Reactivity: Mammalian WB, FACS, ELISA, IHC (p), IP, ICC Host: Mouse Monoclonal C-04 unconjugated
anti-Keratin 18 (KRT18) (AA 53-150) antibodyKRT18 Reactivity: Human, Mouse, Rat, Pig WB, FACS, ELISA, IHC (p), IHC (fro), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated
anti-Keratin 18 (KRT18) (AA 238-396) antibodyKRT18 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Keratin 18 (KRT18) (AA 1-423) antibodyKRT18 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Keratin 18 (KRT18) (AA 238-396) antibodyKRT18 Reactivity: Human WB, IHC, IP, ICC Host: Mouse Monoclonal C2 unconjugated
anti-Keratin 18 (KRT18) (AA 102-396) antibodyKRT18 Reactivity: Human WB, IHC, IF, ELISA Host: Rabbit Polyclonal unconjugated
anti-Keratin 18 (KRT18) (AA 72-380) antibodyKRT18 Reactivity: Rat WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Keratin 18 (KRT18) (AA 401-430), (C-Term) antibodyKRT18 Reactivity: Human, Mouse WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB18746 unconjugated
anti-Keratin 18 (KRT18) (AA 1-150) antibodyKRT18 Reactivity: Human WB, IHC, FACS, ELISA, ICC Host: Mouse Monoclonal 6G12F2 unconjugated
anti-Keratin 18 (KRT18) antibodyKRT18 Reactivity: Human, Dog, Cat WB, IHC, IF, FACS, StM Host: Mouse Monoclonal DE-K18 unconjugated
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- Application Notes
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Western blotting: Recommended dilution: 1-2 μg/mL.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive tissue: colon, skin.
Immunocytochemistry: positive control: MCF-7 human breast adenocarcinoma cell line, HeLa human cervix carcinoma cell line.
ELISA: Positive control: MCF-7 human breast adenocarcinoma cell line, the antibody DC-10 has been tested as the detection antibody in a sandwich ELISA for analysis of Cytokeratin 18 in combination with the antibody C-04 (cat. no. 11-106-C100).
Flow cytometry: Recommended dilution: 1-4 μg/mL. Intracellular staining. - Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Do not freeze.
- Storage
- 4 °C
- Storage Comment
- Store at 2-8°C. Do not freeze.
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: "Primary human hepatocytes on biodegradable poly(l-lactic acid) matrices: a promising model for improving transplantation efficiency with tissue engineering." in: Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society, Vol. 17, Issue 2, pp. 104-14, (2011) (PubMed).
: "Monoclonal antibodies recognizing different epitopes of cytokeratin No.18." in: Folia biologica, Vol. 35, Issue 6, pp. 373-82, (1990) (PubMed).
: "Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread." in: International journal of cancer. Supplement = Journal international du cancer. Supplement, Vol. 3, pp. 50-5, (1989) (PubMed).
: "Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule." in: Hybridoma, Vol. 7, Issue 5, pp. 495-504, (1989) (PubMed).
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: "Primary human hepatocytes on biodegradable poly(l-lactic acid) matrices: a promising model for improving transplantation efficiency with tissue engineering." in: Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society, Vol. 17, Issue 2, pp. 104-14, (2011) (PubMed).
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- Target
- Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))
- Alternative Name
- Cytokeratin 18 (KRT18 Products)
- Synonyms
- CK18 antibody, K18 antibody, Krt1-18 antibody, CYK18 antibody, DreK18 antibody, cb83 antibody, dapk1 antibody, sb:cb83 antibody, wu:fa13f02 antibody, wu:fb36b04 antibody, krt18 antibody, MGC64569 antibody, MGC75922 antibody, KRT18 antibody, CK-18-A antibody, K18-A antibody, NC-11 antibody, cyk18 antibody, k18 antibody, krt18-a antibody, krt18-b antibody, krt18a antibody, xkendob antibody, keratin 18 antibody, keratin 18, type I L homeolog antibody, keratin 18, type I antibody, keratin, type I cytoskeletal 18 antibody, K18, simple type I keratin antibody, Keratin, type I cytoskeletal 18 antibody, keratin 18, type I S homeolog antibody, Krt18 antibody, KRT18 antibody, krt18 antibody, krt18.L antibody, LOC700569 antibody, LOC100136778 antibody, k1c18 antibody, LOC100008885 antibody, krt18.S antibody
- Background
- Keratin 18,Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratin 18 belongs to type I family (acidic cytokeratins).,K18, CK18, CYK18, KRT18
- Gene ID
- 3875
- UniProt
- P05783
- Pathways
- Apoptosis, Caspase Cascade in Apoptosis
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