LAG3 Protein (AA 23-434) (His tag)
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- Target See all LAG3 Proteins
- LAG3 (Lymphocyte-Activation Gene 3 (LAG3))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 23-434
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This LAG3 protein is labelled with His tag.
- Purpose
- Human LAG3/CD223 Protein
- Sequence
- Leu23-Gly434
- Characteristics
- Recombinant Human LAG3/CD223 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu23-Gly434.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human LAG3, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-LAG3 Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA. See testing image for detail.
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Lymphocyte-Activation Gene 3 (LAG3) (AA 23-450) protein (Fc Tag)
LAG3 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Lymphocyte-Activation Gene 3 (LAG3) (AA 23-434) protein (His-Avi Tag,Biotin)LAG3 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Lymphocyte-Activation Gene 3 (LAG3) protein (His tag)LAG3 Origin: Mouse Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE
Lymphocyte-Activation Gene 3 (LAG3) (AA 23-450) (Active) protein (His tag)LAG3 Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Lymphocyte-Activation Gene 3 (LAG3) (AA 18-449) (Active) protein (Fc Tag)LAG3 Origin: Cynomolgus Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Lymphocyte-Activation Gene 3 (LAG3) (Active) protein (His tag)LAG3 Origin: Cynomolgus Host: Human Cells Recombinant > 95 % as determined by reducing SDS-PAGE. Active
Lymphocyte-Activation Gene 3 (LAG3) (AA 23-222) protein (His-IF2DI Tag)LAG3 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Lymphocyte-Activation Gene 3 (LAG3) (Active) protein (His tag)LAG3 Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE Active
Lymphocyte-Activation Gene 3 (LAG3) protein (Fc Tag)LAG3 Origin: Cynomolgus Host: Human Cells Recombinant > 90 % as determined by reducing SDS-PAGE.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- LAG3 (Lymphocyte-Activation Gene 3 (LAG3))
- Alternative Name
- LAG3 (LAG3 Products)
- Synonyms
- CD223 Protein, LAG-3 Protein, Ly66 Protein, lymphocyte activating 3 Protein, lymphocyte-activation gene 3 Protein, LAG3 Protein, Lag3 Protein
- Background
- LAG-3, is a protein which in humans is encoded by the LAG3 gene, which is a cell surface molecule with diverse biologic effects on T cell function. It is an immune checkpoint receptor and as such is the target of various drug development programs by pharmaceutical companies seeking to develop new treatments for cancer and autoimmune disorders.
- Molecular Weight
- 45.5 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
- Pathways
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints
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