PDGFC Protein (Homodimer)
-
- Target See all PDGFC Proteins
- PDGFC (Platelet-Derived Growth Factor C (PDGFC))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- Homodimer
-
Origin
- Parapoxvirus
-
Source
- Escherichia coli (E. coli)
- Sequence
- MGSSHHHHHHSSGLVPRGSHDSTKTWSEVFENSGCKPRPM VFRVHDEHPELTSQRFNPPCVTLMRCGGCCNDESLECVPT EEANVTMQLMGASVSGGNGMQHLSFVEHKKCDCKPPLTTT PPTTTRPPRRRR
- Characteristics
-
Length (AA): 132
Measured in a cell proliferation assay using primary HUVECs. The ED50 for this effect is typically 1-5 ng/mL. - Purity
- > 90 % by SDS-PAGE. Visualized by silver stain
- Endotoxin Level
- < 0.1 ng per µg of ov-VEGF-E
- Top Product
- Discover our top product PDGFC Protein
-
Platelet-Derived Growth Factor C (PDGFC) (AA 236-345) protein (GST tag)
PDGFC Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Platelet-Derived Growth Factor C (PDGFC) (AA 1-345) protein (GST tag)PDGFC Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Platelet-Derived Growth Factor C (PDGFC) (AA 23-345) protein (His tag)Crystallography grade PDGFC Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Platelet-Derived Growth Factor C (PDGFC) (AA 23-345) protein (His tag)Crystallography grade PDGFC Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
-
- Comment
-
Cytokines & Growth Factors
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- The lyophilised ovVEGF-E should be reconstituted in water or medium to a concentration not lower than 50 μg/mL. For long term storage we would recommend to add at least 0.1% human or bovine serum albumin.
- Buffer
- PBS
- Storage
- -20°C
- Storage Comment
- Lyophilised samples are stable for greater than six months at -20 °C to -70 °C. Reconstituted VEGF-E should be stored in working aliquots at -20 °C.
-
- Target
- PDGFC (Platelet-Derived Growth Factor C (PDGFC))
- Alternative Name
- VEGF-E (PDGFC Products)
- Synonyms
- SCDGF Protein, FALLOTEIN Protein, 1110064L01Rik Protein, AI647969 Protein, PDGF-C Protein, platelet derived growth factor C Protein, platelet-derived growth factor, C polypeptide Protein, PDGFC Protein, Pdgfc Protein
- Target Type
- Viral Protein
- Background
-
A DNA sequence encoding the mature variant of ovVEGF-E isolate D1701 (Dehio et al., 1999, GenBank accession No. AF106020) was expressed in E. coli as a 132 amino acid residue fusion protein with an N-terminal His-tag sequence and a thrombin cleavage site. Recombinant VEGF-E homodimer was dimerized in vitro and has a predicted mass of approximately 35 kDa. Based on sequence similarity to VEGF-A, a gene encoding a VEGF homologue has recently been discovered in the genome of Orf virus (OV) (Lyttle et al., 1994). Different isolates of Orf virus show significant amino acid sequence similarity to VEGF-A and described as a viral virulence factor that appears to be derived from captured host genes. All eight cysteine residues of the central cysteine knot motif characteristic of members of the VEGF family are conserved among other residues in the VEGF-E proteins (Dehio et al., 1999, Wise et al., 1999). Alignment of all mammalian VEGF sequences indicated that VEGF-E is distinct from the previously described VEGFs but most closely related to VEGF-A. Like VEGF-A, VEGF-E was found to bind with high affinity to VEGF receptor-2 (KDR) resulting in receptor autophosphorylation, whilst in contrast to VEGF-A, VEGF-E can not bind to VEGF receptor-1 (Flt-1). Furthermore VEGF-E can also not bind to VEGF receptor-3 (FLT-4). Therefore VEGF-E is a potent angiogenic factor selectively binding to VEGF receptor –2/KDR.
Synonyms: Vascular Endothelial Growth Factor-E - Molecular Weight
- 35 kDa
- UniProt
- Q9YMF3
- Pathways
- RTK Signaling, Platelet-derived growth Factor Receptor Signaling
-
