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EPAS1 antibody (C-Term)

Name: EPAS1 antibody (C-Term)
SKU: ABIN540396
Price: 1050 USD
Availability: InStock

EPAS1 Reactivity: Human, Mouse, Rat, Fish, Primate WB, ELISA, IP, FACS, ICC, IHC (p), IF, IHC (fro), ChIP, GS Host: Rabbit Polyclonal unconjugated

Western Blot analysis of (1) normoxic and (2) hypoxic nuclear rat cell lysates with EPAS1 polyclonal antibody .

Immunofluorescent staining of RCC4 cells with EPAS1 polyclonal antibody at a 1:200 dilution against Dylight 488 (Green).

Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human cardiac myocytes with EPAS1 polyclonal antibody .

IHC

Western Blot analysis of (1) Cobalt chloride treated COS-7 nuclear extracts and (2) untreated COS-7 nuclear extracts with EPAS1 polyclonal antibody .

4 images

(6 references)

Catalog No. ABIN540396

Datasheet as PDF

Target See all EPAS1 Antibodies

EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))
Binding Specificity
16

10

9

7

7

6

6

6

6

3

3

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1
C-Term
Reactivity
91

48

42

18

17

3

3

2

2

2

2

2

1

1

1

1

1
Human, Mouse, Rat, Fish, Primate
Host
99

11

1

1
Rabbit
Compatible Secondaries
Clonality
91

21
Polyclonal
Conjugate
56

14

7

5

5

4

2

2

2

2

2

2

2

2

1

1

1

1

1
This EPAS1 antibody is un-conjugated
Application
91

46

31

23

14

13

13

13

13

10

4

4

3

2

1

1

1
Western Blotting (WB), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Chromatin Immunoprecipitation (ChIP), Gel Shift (GS)

Purpose

Rabbit polyclonal antibody raised against synthetic peptide of EPAS1.

Specificity

Specific for HIF-2 alpha/EPAS. Does not cross-react with HIF-1 alpha.

Cross-Reactivity

Fish, Human, Mouse, Primate, Rat

Immunogen

A synthetic peptide corresponding to C-terminus of human and mouse EPAS1/Epas1.

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anti-Endothelial PAS Domain Protein 1 (EPAS1) (AA 339-541) antibody
EPAS1 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Endothelial PAS Domain Protein 1 (EPAS1) (AA 21-336) antibody
EPAS1 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Endothelial PAS Domain Protein 1 (EPAS1) (Ser343) antibody
EPAS1 Reactivity: Human, Mouse, Rat WB, ELISA, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Endothelial PAS Domain Protein 1 (EPAS1) (AA 680-870) antibody
EPAS1 Reactivity: Human WB, ELISA, IHC, FACS, ICC Host: Mouse Monoclonal 1H3E12 unconjugated

anti-Endothelial PAS Domain Protein 1 (EPAS1) (AA 28-347) antibody
EPAS1 Reactivity: Human WB, ELISA, IF Host: Mouse Monoclonal 1G2 unconjugated

anti-Endothelial PAS Domain Protein 1 (EPAS1) antibody
EPAS1 Reactivity: Human WB, ChIP Host: Rabbit Polyclonal unconjugated

anti-Endothelial PAS Domain Protein 1 (EPAS1) (AA 535-631) antibody
EPAS1 Reactivity: Human, Mouse, Rat, Hamster WB, ELISA, IHC, IP, FACS, ICC, IF, ChIP Host: Mouse Monoclonal ep190b unconjugated

anti-Endothelial PAS Domain Protein 1 (EPAS1) (AA 1-130) antibody
EPAS1 Reactivity: Human, Mouse, Rat, Monkey, Sheep WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Endothelial PAS Domain Protein 1 (EPAS1) (AA 543-870) antibody
EPAS1 Reactivity: Human ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Endothelial PAS Domain Protein 1 (EPAS1) (AA 26-347) antibody
EPAS1 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

Application Notes

ChIP (1:10-1:500)
ELISA (1:100-1:2000)
Flow Cytometry
Gel Supershift Assay
Immunocytochemistry (1:100)
Immunofluorescence (1:100)
Immunohistochemistry (Frozen sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sectio

Restrictions

For Research Use only
Format

Liquid

Buffer

In PBS (0.05 % sodium azide)

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
Ruf, Moch, Schraml: "PD-L1 expression is regulated by hypoxia inducible factor in clear cell renal cell carcinoma." in: International journal of cancer, Vol. 139, Issue 2, pp. 396-403, (2016) (PubMed).

Wacker, Sachs, Knaup, Wiesener, Weiske, Huber, Akçetin, Behrens: "Key role for activin B in cellular transformation after loss of the von Hippel-Lindau tumor suppressor." in: Molecular and cellular biology, Vol. 29, Issue 7, pp. 1707-18, (2009) (PubMed).

Lee, Qian, Rey, Wei, Liu, Semenza: "Anthracycline chemotherapy inhibits HIF-1 transcriptional activity and tumor-induced mobilization of circulating angiogenic cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 7, pp. 2353-8, (2009) (PubMed).

Walter, Etienne-Selloum, Brasse, Schleiffer, Bekaert, Vanhoutte, Beretz, Schini-Kerth: "Red wine polyphenols prevent acceleration of neovascularization by angiotensin II in the ischemic rat hindlimb." in: The Journal of pharmacology and experimental therapeutics, Vol. 329, Issue 2, pp. 699-707, (2009) (PubMed).

Lomb, Desouza, Franklin, Freeman et al.: "Prolyl hydroxylase inhibitors depend on extracellular glucose and hypoxia-inducible factor (HIF)-2alpha to inhibit cell death caused by nerve growth factor (NGF) deprivation: evidence that HIF-2alpha ..." in: Molecular pharmacology, Vol. 75, Issue 5, pp. 1198-209, (2009) (PubMed).

Sears, Hoppe, Ebrahem, Anand-Apte: "Prolyl hydroxylase inhibition during hyperoxia prevents oxygen-induced retinopathy." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 50, pp. 19898-903, (2008) (PubMed).
Target

EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Alternative Name

HIF2A / HIF2 alpha (EPAS1 Products)

Gene ID

2034, 13819

Pathways

Signaling Events mediated by VEGFR1 and VEGFR2, Warburg Effect