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ARHGDIA antibody (AA 1-204)

Name: ARHGDIA antibody (AA 1-204)
Brand: antibodies-online
SKU: ABIN969501
Price: 511.5 USD
Availability: InStock

ARHGDIA Reactivity: Human, Mouse, Monkey WB, ELISA, FACS, ICC Host: Mouse Monoclonal 2G3 unconjugated

Malfunctioning Rho-GTPase network in kidneys from Memo cKO (cKO) mice.

Black line: Control Antigen (100 ng), Purple line: Antigen(10 ng), Blue line: Antigen (50 ng), Red line: Antigen (100 ng),

ELISA

Western blot analysis using ARHGDIA mouse mAb against Jurkat (1), HeLa (2), NIH3T3 (3), C6 (4), K562 (5), and COS7 (6) cell lysate.

Immunofluorescence analysis of HepG2 cells using ARHGDIA mouse mAb (green).

Flow cytometric analysis of HeLa cells using ARHGDIA mouse mAb (green) and negative control (red).

FACS

Western blot analysis using ARHGDIA mAb against human ARHGDIA recombinant protein.

6 images

(3 references)

Catalog No. ABIN969501

Datasheet as PDF

Target See all ARHGDIA Antibodies

ARHGDIA (rho GDP Dissociation Inhibitor (GDI) alpha (ARHGDIA))
Binding Specificity
15

8

5

5

4

2

2

2

1

1

1

1

1

1
AA 1-204
Reactivity
60

20

17

4

1
Human, Mouse, Monkey
Host
54

7

1
Mouse
Compatible Secondaries
Clonality
56

4
Monoclonal
Conjugate
34

4

4

3

2

2

2

2

2

2

1

1

1

1

1
This ARHGDIA antibody is un-conjugated
Application
35

24

13

13

13

8

6

5

3

2

1

1

1

1
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC)

Purification

purified

Immunogen

Purified recombinant fragment of human ARHGDIA (AA: FULL(1-204)) expressed in E. coli.

Clone

2G3

Isotype

IgG1
anti-rho GDP Dissociation Inhibitor (GDI) alpha (ARHGDIA) (AA 1-204) antibody
ARHGDIA Reactivity: Human WB, IP Host: Rabbit Polyclonal unconjugated

anti-rho GDP Dissociation Inhibitor (GDI) alpha (ARHGDIA) (AA 1-204) antibody
ARHGDIA Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated

anti-rho GDP Dissociation Inhibitor (GDI) alpha (ARHGDIA) (AA 1-204) antibody
ARHGDIA Reactivity: Human, Mouse, Monkey WB, ELISA, FACS, IF/ICC Host: Mouse Monoclonal unconjugated

anti-rho GDP Dissociation Inhibitor (GDI) alpha (ARHGDIA) (N-Term) antibody
ARHGDIA Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-rho GDP Dissociation Inhibitor (GDI) alpha (ARHGDIA) (AA 112-140), (C-Term) antibody
ARHGDIA Reactivity: Human WB, IHC (p), FACS Host: Rabbit Polyclonal RB20512 unconjugated

anti-rho GDP Dissociation Inhibitor (GDI) alpha (ARHGDIA) antibody
ARHGDIA Reactivity: Human, Mouse, Rat WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-rho GDP Dissociation Inhibitor (GDI) alpha (ARHGDIA) antibody
ARHGDIA Reactivity: Human WB Host: Rabbit Polyclonal unconjugated

anti-rho GDP Dissociation Inhibitor (GDI) alpha (ARHGDIA) (AA 2-204) antibody
ARHGDIA Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-rho GDP Dissociation Inhibitor (GDI) alpha (ARHGDIA) (AA 1-204) antibody
ARHGDIA Reactivity: Human WB Host: Mouse Polyclonal unconjugated

anti-rho GDP Dissociation Inhibitor (GDI) alpha (ARHGDIA) antibody
ARHGDIA Reactivity: Human, Mouse, Rat WB, IF Host: Rabbit Polyclonal unconjugated

Application Notes

ELISA: 1:10000, WB: 1:500 - 1:2000, ICC: 1:200 - 1:1000, FCM: 1:200 - 1:400

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified antibody in PBS with 0.05 % sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C/-20 °C

Storage Comment

4°C, -20°C for long term storage
Bartos, Ramakrishnan, Braga-Lagache, Hänzi, Durussel, Prakash Sridharan, Zhu, Sheehan, Hynes, Bonny, Moor: "Renal FGF23 signaling depends on redox protein Memo1 and promotes orthovanadate-sensitive protein phosphotyrosyl phosphatase activity." in: Journal of cell communication and signaling, (2022) (PubMed).

Boulter, Garcia-Mata, Guilluy, Dubash, Rossi, Brennwald, Burridge: "Regulation of Rho GTPase crosstalk, degradation and activity by RhoGDI1." in: Nature cell biology, Vol. 12, Issue 5, pp. 477-83, (2010) (PubMed).

Ronneburg, Span, Kantelhardt, Dittmer, Schunke, Holzhausen, Sweep, Dittmer: "Rho GDP dissociation inhibitor alpha expression correlates with the outcome of CMF treatment in invasive ductal breast cancer." in: International journal of oncology, Vol. 36, Issue 2, pp. 379-86, (2009) (PubMed).
Target

ARHGDIA (rho GDP Dissociation Inhibitor (GDI) alpha (ARHGDIA))

Alternative Name

ARHGDIA (ARHGDIA Products)

Synonyms

GDIA1 antibody, NPHS8 antibody, RHOGDI antibody, RHOGDI-1 antibody, Bles01 antibody, zgc:55554 antibody, zgc:77681 antibody, wu:fa96g11 antibody, ARHGDIA antibody, NV15502 antibody, 5330430M07Rik antibody, Gdi-1 antibody, Rho-GDI antibody, RhoDGI antibody, RhoGDI-1 antibody, arhgdia antibody, MGC84535 antibody, gdia1 antibody, rhogdi antibody, rhogdi-1 antibody, rhogdi1 antibody, Rho GDP dissociation inhibitor alpha antibody, Rho GDP dissociation inhibitor (GDI) alpha antibody, rho GDP-dissociation inhibitor 2 antibody, Rho GDP dissociation inhibitor (GDI) alpha L homeolog antibody, ARHGDIA antibody, Arhgdia antibody, arhgdia antibody, LOC100120432 antibody, arhgdia.L antibody

Background

Description: Aplysia Ras-related homologs (ARHs), also called Rho genes, belong to the RAS gene superfamily encoding small guanine nucleotide exchange (GTP/GDP) factors. The ARH proteins may be kept in the inactive, GDP-bound state by interaction with GDP dissociation inhibitors, such as ARHGDIA
Aliases: GDIA1,RHOGDI,RHOGDI-1

Molecular Weight

26 kDa

Gene ID

396

HGNC

396

Pathways

Neurotrophin Signaling Pathway