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RGS7BP Protein (AA 1-257) (His tag)

Crystallography grade RGS7BP Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, Crys, SDS, WB
Catalog No. ABIN3086870
  • Target See all RGS7BP Proteins
    RGS7BP (Regulator of G Protein Signaling 7 Binding Protein (RGS7BP))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-257
    Origin
    • 2
    • 1
    • 1
    Human
    Source
    • 3
    • 1
    Insect Cells
    Purification tag / Conjugate
    This RGS7BP protein is labelled with His tag.
    Application
    ELISA, Crystallization (Crys), SDS-PAGE (SDS), Western Blotting (WB)
    Sequence
    MSSAPNGRKK RPSRSTRSSI FQISKPPLQS GDWERRGSGS ESAHKTQRAL DDCKMLVQEF NTQVALYREL VISIGDVSVS CPSLRAEMHK TRTKGCEMAR QAHQKLAAIS GPEDGEIHPE ICRLYIQLQC CLEMYTTEML KSICLLGSLQ FHRKGKEPGG GTKSLDCKIE ESAETPALED SSSSPVDSQQ HSWQVSTDIE NTERDMREMK NLLSKLRETM PLPLKNQDDS SLLNLTPYPL VRRRKRRFFG LCCLISS
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human RGS7BP Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    RGS7BP (Regulator of G Protein Signaling 7 Binding Protein (RGS7BP))
    Alternative Name
    RGS7BP (RGS7BP Products)
    Synonyms
    A930030I01Rik Protein, AI842293 Protein, D13Bwg1146e Protein, R7bp Protein, R7BP Protein, RGD1309360 Protein, zgc:154140 Protein, fj83a05 Protein, rgs7bp Protein, wu:fj83a05 Protein, zgc:110002 Protein, regulator of G-protein signalling 7 binding protein Protein, regulator of G protein signaling 7 binding protein Protein, regulator of G-protein signaling 7 binding protein Protein, regulator of G protein signaling 7 binding protein a Protein, regulator of G protein signaling 7 binding protein b Protein, regulator of G-protein signaling 7 binding protein S homeolog Protein, Rgs7bp Protein, RGS7BP Protein, rgs7bpa Protein, rgs7bpb Protein, rgs7bp.S Protein
    Background
    Regulator of G protein-coupled receptor (GPCR) signaling. Regulatory subunit of the R7-Gbeta5 complexes that acts by controlling the subcellular location of the R7-Gbeta5 complexes. When palmitoylated, it targets the R7-Gbeta5 complexes to the plasma membrane, leading to inhibit G protein alpha subunits. When it is unpalmitoylated, the R7-Gbeta5 complexes undergo a nuclear/cypolasmic shuttling. May also act by controlling the proteolytic stability of R7 proteins, probably by protecting them from degradation. {ECO:0000250|UniProtKB:Q8BQP9}.
    Molecular Weight
    29.9 kDa Including tag.
    UniProt
    Q6MZT1
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