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Arylsulfatase A antibody (Internal Region)

Name: Arylsulfatase A antibody (Internal Region)
Brand: antibodies-online
SKU: ABIN238536
Price: 469.28 USD
Availability: InStock

Verified ARSA Reactivity: Human, Mouse, Rat WB, ELISA, IHC, FACS, IF Host: Goat Polyclonal unconjugated

ABIN238536 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.

FACS

ABIN238536 (5µg/ml) staining of paraffin embedded Human Testis.

IHC

ABIN238536 (5µg/ml) staining of paraffin embedded Human Cortex.

IHC

ABIN238536 (1 μg/mL) staining of Mouse Testes lysate (A) + peptide (B) and (0.

ABIN238536 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.

6 images

(4 references)

Catalog No. ABIN238536

Datasheet as PDF

Target See all Arylsulfatase A (ARSA) Antibodies

Arylsulfatase A (ARSA)
Binding Specificity
19

15

6

5

4

4

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
Internal Region
Reactivity
56

35

33

4

4

3

2

2

1

1
Human, Mouse, Rat
Host
55

16
Goat
Compatible Secondaries
Clonality
57

14
Polyclonal
Conjugate
35

4

4

3

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1
This Arylsulfatase A antibody is un-conjugated
Application
54

21

13

13

13

9

8

7

5

4

4

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Purpose

Arylsulfatase A

Sequence

YDLSKDPGEN YN

Cross-Reactivity

Human, Mouse, Rat

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade

Verified

Immunogen

Peptide with sequence C-YDLSKDPGENYN, from the internal region of the protein sequence according to NP_000478.2.

Isotype

IgG
anti-Arylsulfatase A (ARSA) antibody
ARSA Reactivity: Human WB, IHC, ICC, IP Host: Rabbit Polyclonal unconjugated

anti-Arylsulfatase A (ARSA) (Internal Region) antibody
ARSA Reactivity: Human, Mouse, Rat WB, ELISA, IHC, ICC, IF Host: Rabbit Polyclonal unconjugated

anti-Arylsulfatase A (ARSA) antibody
ARSA Reactivity: Human, Mouse ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-Arylsulfatase A (ARSA) (AA 1-507) antibody
ARSA Reactivity: Human WB, IP Host: Rabbit Polyclonal unconjugated

anti-Arylsulfatase A (ARSA) (AA 1-507) antibody
ARSA Reactivity: Human WB, IHC (p) Host: Mouse Polyclonal unconjugated

anti-Arylsulfatase A (ARSA) (AA 210-509) antibody
ARSA Reactivity: Human WB Host: Rabbit Polyclonal unconjugated

anti-Arylsulfatase A (ARSA) (AA 21-295) antibody
ARSA Reactivity: Human WB, IHC, ICC, IP Host: Rabbit Polyclonal unconjugated

anti-Arylsulfatase A (ARSA) (AA 454-482), (C-Term) antibody
ARSA Reactivity: Human, Mouse, Rat WB, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Arylsulfatase A (ARSA) antibody
ARSA Reactivity: Human, Mouse, Rat WB, IHC (p), FACS Host: Rabbit Polyclonal unconjugated

anti-Arylsulfatase A (ARSA) (AA 368-412) antibody
ARSA Reactivity: Mouse, Rat WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Application Notes

Immunohistochemistry: Paraffin embedded Human Brain (Cortex) and Testis. Recommended concentration: 5 μg/mL.
Western Blot: Approx 60-65Da band observed in Mouse and Rat Testis lysates (calculated MW of 53.7 kDa according to Mouse NP_033843.2 and Rat NP_001030105.2,). This molecular weight is routinely observed by other sources and was successfully blocked by inc
Peptide ELISA: antibody detection limit dilution 1:32000.

Comment

Immunofluorescence: Strong expression of the protein seen in the Golgi apparatus of HeLa cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10u

Restrictions

For Research Use only
Format

Liquid

Concentration

0.5 mg/mL

Buffer

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Minimize freezing and thawing.

Storage

-20 °C

Storage Comment

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Kanbe, Kamijo, Nakajima, Tanaka, Sugiyama, Wang, Fang, Hara, Gonzalez, Aoyama: "Chronic ethanol consumption decreases serum sulfatide levels by suppressing hepatic cerebroside sulfotransferase expression in mice." in: Archives of toxicology, Vol. 88, Issue 2, pp. 367-79, (2014) (PubMed).

Kimura, Nakajima, Kamijo, Tanaka, Wang, Hara, Sugiyama, Tanaka, Gonzalez, Aoyama: "Hepatic Cerebroside Sulfotransferase Is Induced by PPAR? Activation in Mice." in: PPAR research, Vol. 2012, pp. 174932, (2012) (PubMed).

Kiebish, Young, Lehman, Han: "Chronic caloric restriction attenuates a loss of sulfatide content in PGC-1?-/- mouse cortex: a potential lipidomic role of PGC-1? in neurodegeneration." in: Journal of lipid research, Vol. 53, Issue 2, pp. 273-81, (2012) (PubMed).

Zhang, Nakajima, Kamijo, Li, Hu, Kannagi, Kyogashima, Aoyama, Hara: "Acute kidney injury induced by protein-overload nephropathy down-regulates gene expression of hepatic cerebroside sulfotransferase in mice, resulting in reduction of liver and serum sulfatides." in: Biochemical and biophysical research communications, Vol. 390, Issue 4, pp. 1382-8, (2009) (PubMed).
Target

Arylsulfatase A (ARSA)

Alternative Name

ARSA (ARSA Products)

Synonyms

ARSA antibody, zgc:101575 antibody, arsa antibody, AS-A antibody, ASA antibody, AW212749 antibody, As-2 antibody, As2 antibody, TISP73 antibody, MLD antibody, mld antibody, arylsulfatase A antibody, arylsulfatase antibody, arylsulfatase A, gene 1 S homeolog antibody, ARSA antibody, arsa antibody, arsA antibody, RB6599 antibody, Arsa antibody, arsa.1.S antibody

Background

ARSA, arylsulfatase A, MLD, cerebroside-sulfatase, cerebroside 3-sulfatase

Gene ID

410, 11883, 315222

NCBI Accession

NP_000478