CIITA antibody (AA 945-1130)
(1 reference)-
- Target See all CIITA Antibodies
- CIITA (Class II, Major Histocompatibility Complex, Transactivator (CIITA))
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Binding Specificity
- AA 945-1130
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This CIITA antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purpose
- Rabbit IgG polyclonal antibody for MHC class II transactivator(CIITA) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
- Cross-Reactivity (Details)
- No cross reactivity with other proteins.
- Characteristics
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Rabbit IgG polyclonal antibody for MHC class II transactivator(CIITA) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: class II, major histocompatibility complex, transactivator
Protein Name: MHC class II transactivator - Purification
- Immunogen affinity purified.
- Immunogen
- E.coli-derived human CIITA recombinant protein (Position: E945-R1130). Human CIITA shares 84.4% amino acid (aa) sequence identity with mouse CIITA.
- Isotype
- IgG
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anti-Class II, Major Histocompatibility Complex, Transactivator (CIITA) (Internal Region) antibody
CIITA Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Class II, Major Histocompatibility Complex, Transactivator (CIITA) (AA 1) antibodyCIITA Reactivity: Pig WB Host: Rabbit Polyclonal unconjugated
anti-Class II, Major Histocompatibility Complex, Transactivator (CIITA) (AA 841-890) antibodyCIITA Reactivity: Mouse, Dog WB Host: Rabbit Polyclonal unconjugated
anti-Class II, Major Histocompatibility Complex, Transactivator (CIITA) (C-Term) antibodyCIITA Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Monkey WB Host: Rabbit Polyclonal unconjugated
anti-Class II, Major Histocompatibility Complex, Transactivator (CIITA) (AA 650-750) antibodyCIITA Reactivity: Human WB Host: Rabbit Polyclonal unconjugated
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- Application Notes
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. - Comment
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Concentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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: "Autoregulatory loop between TGF-β1/miR-411-5p/SPRY4 and MAPK pathway in rhabdomyosarcoma modulates proliferation and differentiation." in: Cell death & disease, Vol. 6, pp. e1859, (2016) (PubMed).
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: "Autoregulatory loop between TGF-β1/miR-411-5p/SPRY4 and MAPK pathway in rhabdomyosarcoma modulates proliferation and differentiation." in: Cell death & disease, Vol. 6, pp. e1859, (2016) (PubMed).
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- Target
- CIITA (Class II, Major Histocompatibility Complex, Transactivator (CIITA))
- Alternative Name
- CIITA (CIITA Products)
- Synonyms
- CIITA antibody, C2ta antibody, EG669998 antibody, Gm9475 antibody, C2TA antibody, CIITAIV antibody, MHC2TA antibody, NLRA antibody, Mhc2ta antibody, class II, major histocompatibility complex, transactivator antibody, class II major histocompatibility complex transactivator antibody, MHC class II transactivator antibody, class II transactivator antibody, CIITA antibody, LOC710761 antibody, ciita antibody, LOC100443575 antibody, Ciita antibody, LOC100736732 antibody
- Background
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CIITA is a human gene which is mapped to 16p13. This gene encodes a protein with an acidic transcriptional activation domain, 4 LRRs (leucine-rich repeats) and a GTP binding domain. The protein is located in the nucleus and acts as a positive regulator of class II major histocompatibility complex gene transcription, and is referred to as the "master control factor" for the expression of these genes. Also, the protein binds GTP and uses GTP binding to facilitate its own transport into the nucleus. Once in the nucleus it does not bind DNA but rather uses an intrinsic acetyltransferase (AT) activity to act in a coactivator-like fashion. Mutations in this gene have been associated with bare lymphocyte syndrome type II (also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency), increased susceptibility to rheumatoid arthritis, multiple sclerosis, and possibly myocardial infarction. Several transcript variants encoding different isoforms have been found for this gene.
Synonyms: C2TA | CIITA | CIITA IV | MHC2TA | NLRA | P33076 - Gene ID
- 4261
- UniProt
- P33076
- Pathways
- Cancer Immune Checkpoints
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