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NRF2 antibody (pSer40)

Name: NRF2 antibody (pSer40)
SKU: ABIN676673
Price: 384.62 USD
Availability: InStock

NFE2L2 Reactivity: Human, Mouse, Rat, Pig WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody (ABIN676673), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

IHC

Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody , Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

IHC (p)

Formalin-fixed and paraffin embedded rat tongue tissue labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody (ABIN676673), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

SDS

Formalin-fixed and paraffin embedded rat tongue tissue labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody , Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

IHC (p)

Formalin-fixed and paraffin embedded human colon carcinoma tissue labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody (ABIN676673), Unconjugated 1:200 followed by conjugation to the secondary Cy5 antibody and DAPIstaining

L1 mouse heart, L2 mouse muscle lysates probed (ABIN676673) at 1:200 in 4 °C.

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody, Unconjugated (ABIN676673) at 1:200 followed by conjugation to the secondary antibody and DAB staining

IHC

7 images

(19 references)

Catalog No. ABIN676673

Datasheet as PDF

Target See all NRF2 (NFE2L2) Antibodies

NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))
Binding Specificity
21

15

9

8

8

7

7

4

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pSer40
Reactivity
120

56

54

5

3

3

2

2

1

1

1

1

1

1
Human, Mouse, Rat, Pig
Host
113

9

1
Rabbit
Compatible Secondaries
Clonality
104

19
Polyclonal
Conjugate
58

10

7

6

4

4

4

4

4

4

4

4

1

1

1

1

1

1

1

1

1

1
This NRF2 antibody is un-conjugated
Application
96

51

31

30

21

17

14

14

13

10

6

2

2

2

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity

Human, Mouse, Pig, Rat

Predicted Reactivity

Dog,Cow,Chicken

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic phosphopeptide derived from human Nrf2 around the phosphorylation site of Ser40 [DF(p-S)QR]

Isotype

IgG
anti-Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2) (AA 430-480) antibody
NFE2L2 Reactivity: Human, Mouse, Rat WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2) (Internal Region) antibody
NFE2L2 Reactivity: Human, Rat WB, IF, ICC, IP, IHC (p), ChIP Host: Rabbit Polyclonal unconjugated

anti-Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2) (AA 366-605) antibody
NFE2L2 Reactivity: Human WB, IHC, IF, IP Host: Rabbit Polyclonal unconjugated

anti-Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2) (AA 382-604) antibody
NFE2L2 Reactivity: Rat WB, IHC, ICC, IP Host: Rabbit Polyclonal unconjugated

anti-Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2) (acLys599) antibody
NFE2L2 Reactivity: Human, Mouse, Rat WB, ELISA, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2) (N-Term) antibody
NFE2L2 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2) antibody
NFE2L2 Reactivity: Human, Mouse, Rat IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2) (AA 356-589) antibody
NFE2L2 Reactivity: Human WB, ELISA, IHC Host: Mouse Monoclonal 1A6G6 unconjugated

anti-Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2) (AA 445-458) antibody
Verified NFE2L2 Reactivity: Human WB, ELISA, IF, FACS Host: Goat Polyclonal unconjugated

anti-Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2) (pSer40) antibody
NFE2L2 Reactivity: Human, Mouse, Rat WB, IHC (p), FACS, IF (cc) Host: Rabbit Monoclonal 7G4 unconjugated

Application Notes

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Ghosh, Abdo, Zhao, Wu, Shi, Lo, Chenier, Alquier, Filep, Ingelfinger, Zhang, Chan: "Insulin Inhibits Nrf2 Gene Expression via Heterogeneous Nuclear Ribonucleoprotein F/K in Diabetic Mice." in: Endocrinology, Vol. 158, Issue 4, pp. 903-919, (2017) (PubMed).

Allard, Perez, Fukui, Carpenter, Ingram, de Cabo: "Prolonged metformin treatment leads to reduced transcription of Nrf2 and neurotrophic factors without cognitive impairment in older C57BL/6J mice." in: Behavioural brain research, Vol. 301, pp. 1-9, (2016) (PubMed).

Yen, Chen, Jayakumar, Lu, Hsieh, Hsu, Yang, Chang, Lin, Lin, Sheu et al.: "Andrographolide stimulates p38 mitogen-activated protein kinase-nuclear factor erythroid-2-related factor 2-heme oxygenase 1 signaling in primary cerebral endothelial cells for definite protection ..." in: Translational research : the journal of laboratory and clinical medicine, Vol. 170, pp. 57-72, (2016) (PubMed).

Ko, Rho, Jeon, Kim, Woo, Kim, Kim: "Crude Preparations of Helicobacter pylori Outer Membrane Vesicles Induce Upregulation of Heme Oxygenase-1 via Activating Akt-Nrf2 and mTOR-IκB Kinase-NF-κB Pathways in Dendritic Cells." in: Infection and immunity, Vol. 84, Issue 8, pp. 2162-74, (2016) (PubMed).

Ko, Rho, Jeon, Kim, Woo, Lee, Kim: "Bacteroides fragilis Enterotoxin Upregulates Heme Oxygenase-1 in Intestinal Epithelial Cells via a Mitogen-Activated Protein Kinase- and NF-κB-Dependent Pathway, Leading to Modulation of Apoptosis." in: Infection and immunity, Vol. 84, Issue 9, pp. 2541-54, (2016) (PubMed).

Kim, Kim, Jung, Yang, Park, Jun: "EX4 stabilizes and activates Nrf2 via PKCδ, contributing to the prevention of oxidative stress-induced pancreatic beta cell damage." in: Toxicology and applied pharmacology, Vol. 315, pp. 60-69, (2016) (PubMed).

Cheng, Cheng, Lee, Chung, Chang: "Resveratrol protects against methylglyoxal-induced hyperglycemia and pancreatic damage in vivo." in: Nutrients, Vol. 7, Issue 4, pp. 2850-65, (2016) (PubMed).

Chang, Wu, Xu, Liao, Wu, Cheng: "Scopoletin Protects against Methylglyoxal-Induced Hyperglycemia and Insulin Resistance Mediated by Suppression of Advanced Glycation Endproducts (AGEs) Generation and Anti-Glycation." in: Molecules (Basel, Switzerland), Vol. 20, Issue 2, pp. 2786-801, (2015) (PubMed).

Kaneko, Takayanagi, Nagasaki, Kodani, Nakashima, Mori, Suzuki, Itoh, Kondo, Nagatsu, Ota, Ota: "Aripiprazole increases NAD(P)H-quinone oxidoreductase-1 and heme oxygenase-1 in PC12 cells." in: Journal of neural transmission (Vienna, Austria : 1996), Vol. 122, Issue 6, pp. 757-72, (2015) (PubMed).

Santofimia-Castaño, Clea Ruy, Garcia-Sanchez, Jimenez-Blasco, Fernandez-Bermejo, Bolaños, Salido, Gonzalez: "Melatonin induces the expression of Nrf2-regulated antioxidant enzymes via PKC and Ca(2+) influx activation in mouse pancreatic acinar cells." in: Free radical biology & medicine, (2015) (PubMed).

Mo, Wang, Zhang, Numazawa, Tang, Tang, Han, Li, Yang, Wang, Wei, Xiao: "The crosstalk between Nrf2 and AMPK signal pathways is important for the anti-inflammatory effect of berberine in LPS-stimulated macrophages and endotoxin-shocked mice." in: Antioxidants & redox signaling, Vol. 20, Issue 4, pp. 574-88, (2014) (PubMed).

Kakehashi, Kato, Ishii, Wei, Morimura, Fukushima, Wanibuchi: "Valerian inhibits rat hepatocarcinogenesis by activating GABA(A) receptor-mediated signaling." in: PLoS ONE, Vol. 9, Issue 11, pp. e113610, (2014) (PubMed).

Li, Guo, Xi, Yang, Zhou, Yin, Hai, Li, Qin: "Saponins from Aralia taibaiensis attenuate D-galactose-induced aging in rats by activating FOXO3a and Nrf2 pathways." in: Oxidative medicine and cellular longevity, Vol. 2014, pp. 320513, (2014) (PubMed).

Patwardhan, Sharma, Checker, Sandur: "Mitigation of radiation-induced hematopoietic injury via regulation of cellular MAPK/phosphatase levels and increasing hematopoietic stem cells." in: Free radical biology & medicine, Vol. 68, pp. 52-64, (2014) (PubMed).

Lee, Hsu, Hsu, Pan: "Dimerumic acid attenuates receptor for advanced glycation endproducts signal to inhibit inflammation and diabetes mediated by Nrf2 activation and promotes methylglyoxal metabolism into d-lactic acid." in: Free radical biology & medicine, Vol. 60, pp. 7-16, (2013) (PubMed).

Hsu, Lee, Li, Hsu, Pan: "Monascin and AITC attenuate methylglyoxal-induced PPAR? phosphorylation and degradation through inhibition of the oxidative stress/PKC pathway depending on Nrf2 activation." in: Journal of agricultural and food chemistry, Vol. 61, Issue 25, pp. 5996-6006, (2013) (PubMed).

Lee, Lee, Cheng, Chang, Hsu, Wu: "Graptopetalum paraguayense and resveratrol ameliorates carboxymethyllysine (CML)-induced pancreas dysfunction and hyperglycemia." in: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol. 62, pp. 492-8, (2013) (PubMed).

Lee, Hsu, Chang, Kuo, Hsu, Pan: "Ankaflavin: a natural novel PPAR? agonist upregulates Nrf2 to attenuate methylglyoxal-induced diabetes in vivo." in: Free radical biology & medicine, Vol. 53, Issue 11, pp. 2008-16, (2012) (PubMed).
Target

NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))

Alternative Name

Nrf2 (NFE2L2 Products)

Synonyms

Nrf2 antibody, nfe2l2 antibody, wu:fc15g09 antibody, wu:fj67e03 antibody, NF-E2R2 antibody, NRF2 antibody, AI194320 antibody, nuclear factor, erythroid 2-like 2a antibody, nuclear factor, erythroid 2 like 2 S homeolog antibody, nuclear factor, erythroid 2 like 2 antibody, nuclear factor, erythroid derived 2, like 2 antibody, nuclear factor, erythroid 2-like 2 antibody, nfe2l2a antibody, nfe2l2.S antibody, NFE2L2 antibody, Nfe2l2 antibody

Background

Synonyms: NRF2, Nuclear factor erythroid 2-related factor 2, NF-E2-related factor 2, NFE2-related factor 2, HEBP1, Nuclear factor, erythroid derived 2, like 2, NFE2L2
Background: Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.

Gene ID

4780

UniProt

Q16236

Pathways

ER-Nucleus Signaling, Negative Regulation of intrinsic apoptotic Signaling