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RAD51 antibody (AA 81-180)

Name: RAD51 antibody (AA 81-180)
SKU: ABIN4913431
Price: 384.62 USD
Availability: InStock

RAD51 Reactivity: Human, Mouse, Rat WB, ELISA, IHC (p), IF (cc), IF (p), ICC, IHC (fro) Host: Rabbit Polyclonal unconjugated

Mouse bone lysates probed with Rad51 Polyclonal Antibody, Unconjugated at 1:300 overnight at 4˚C.

HEK 293 stained with Rad51 Polyclonal Antibody, Unconjugated at 1:500 in PBS and incubated for one hour at room temperature, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

IF (cc)

Paraformaldehyde-fixed, paraffin embedded human colon cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (BSA or normal goat serum) at 37°C for 20min, Antibody incubation with Rad51 Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

IHC (p)

3 images

(2 references)

Catalog No. ABIN4913431

Datasheet as PDF

Target See all RAD51 Antibodies

RAD51 (DNA Repair Protein Homolog 1 (RAD51))
Binding Specificity
18

14

10

8

7

6

5

5

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 81-180
Reactivity
122

85

43

4

3

3

3

3

2

2

2

1

1

1

1
Human, Mouse, Rat
Host
104

21

1
Rabbit
Compatible Secondaries
Clonality
98

28
Polyclonal
Conjugate
70

6

5

5

5

5

5

5

4

3

2

2

2

2

2

2

1
This RAD51 antibody is un-conjugated
Application
92

38

28

28

23

23

20

16

10

8

7

4

2

2

2

2

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity

Human, Mouse, Rat

Predicted Reactivity

Cow,Pig,Horse,Rabbit

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human Rad51

Isotype

IgG

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RAD51 Reactivity: Human IHC, FACS, ISt, StM Host: Mouse Monoclonal RAD51-2702 unconjugated

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RAD51 Reactivity: Human WB, PLA Host: Rabbit Polyclonal unconjugated

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RAD51 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 2E5-E5 unconjugated

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RAD51 Reactivity: Human WB, ELISA, IP Host: Mouse Monoclonal 4C11-B4 unconjugated

anti-DNA Repair Protein Homolog 1 (RAD51) antibody
RAD51 Reactivity: Human, Mouse WB, ELISA, IP, IF Host: Chicken Polyclonal unconjugated

Application Notes

WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Qi, Jiang, Wang, Yang, Li, He, Yu: "Sensitization of tamoxifen-resistant breast cancer cells by Z-ligustilide through inhibiting autophagy and accumulating DNA damages." in: Oncotarget, Vol. 8, Issue 17, pp. 29300-29317, (2018) (PubMed).
Target

RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Alternative Name

Rad51 (RAD51 Products)

Synonyms

BRCC5 antibody, HRAD51 antibody, HsRad51 antibody, HsT16930 antibody, MRMV2 antibody, RAD51A antibody, RECA antibody, CRAD51 antibody, wu:fb38e12 antibody, zgc:77754 antibody, RGD1563603 antibody, ATRAD51 antibody, F22D1.20 antibody, F22D1_20 antibody, RAS associated with diabetes protein 51 antibody, AV304093 antibody, Rad51a antibody, Reca antibody, brcc5 antibody, rad51 antibody, rad51a antibody, reca antibody, xrad51 antibody, RAD51 recombinase antibody, RAS associated with diabetes protein 51 antibody, RAD51 recombinase L homeolog antibody, RAD51 antibody, rad51 antibody, Rad51 antibody, rad51.L antibody

Background

Synonyms: RECA, BRCC5, FANCR, MRMV2, HRAD51, RAD51A, HsRad51, HsT16930, DNA repair protein RAD51 homolog 1, RAD51 homolog A, RAD51
Background: Participates in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA and forms helical nucleoprotein filaments. Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.

Gene ID

5888

UniProt

Q06609

Pathways

DNA Damage Repair