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SMAD2 antibody (N-Term)

Name: SMAD2 antibody (N-Term)
SKU: ABIN2780721
Price: 418.92 USD
Availability: InStock

SMAD2 Reactivity: Human, Mouse, Rat, Cow, Rabbit, Zebrafish (Danio rerio), Sheep, Dog, Horse, Guinea Pig WB Host: Rabbit Polyclonal unconjugated

WB Suggested Anti-SMAD2 Antibody Titration: 2.

Lanes: Lane1: 2ug HEK293lysate Lane2: 2ug SMAD2 transfected HEK293 lysate Primary Antibody Dilution: 1:1000 Secondary Antibody: Anti-rabbit HRP Secondary Antibody Dilution: 1:5000 Gene Name: SMAD2 Submitted by: Dominique Alfandari, University of Massachusetts

Host: Rat Target Name: SMAD2 Sample Tissue: Rat Liver Antibody Dilution: 1ug/ml

3 images

Catalog No. ABIN2780721

Datasheet as PDF

Target See all SMAD2 Antibodies

SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))
Binding Specificity
47

37

30

26

21

16

9

8

7

7

7

6

4

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
N-Term
Reactivity
235

149

116

11

11

9

8

7

7

7

6

5

4

4

3

3

1

1
Human, Mouse, Rat, Cow, Rabbit, Zebrafish (Danio rerio), Sheep, Dog, Horse, Guinea Pig
Host
238

10
Rabbit
Compatible Secondaries
Clonality
212

36
Polyclonal
Conjugate
126

18

12

10

8

8

8

8

8

7

7

5

5

5

5

5

3
This SMAD2 antibody is un-conjugated
Application
210

101

64

53

52

44

41

39

18

14

10

10

5

2

2

1

1
Western Blotting (WB)

Sequence

VVKRLLGWKK SAGGSGGAGG GEQNGQEEKW CEKAVKSLVK KLKKTGRLDE

Predicted Reactivity

Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 100%

Characteristics

This is a rabbit polyclonal antibody against SMAD2. It was validated on Western Blot using a cell lysate as a positive control.

Purification

Protein A purified

Immunogen

The immunogen is a synthetic peptide directed towards the N terminal region of human SMAD2

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anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) antibody
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anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (AA 181-280) antibody
SMAD2 Reactivity: Human WB, ELISA, IF, IHC (p) Host: Mouse Monoclonal 3G9 unconjugated

anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (AA 2-467) antibody
SMAD2 Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (C-Term) antibody
SMAD2 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (pSer423), (pSer425), (pSer465), (pSer467) antibody
SMAD2 Reactivity: Human, Mouse, Rat WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) antibody
KO Validated SMAD2 Reactivity: Human, Mouse, Rat WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (pSer467) antibody
SMAD2 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (AA 181-280) antibody
SMAD2 Reactivity: Human WB, ELISA, IF, IHC (p) Host: Mouse Monoclonal 4D10 unconjugated

anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) antibody
KO Validated SMAD2 Reactivity: Human WB, IF, IP Host: Rabbit Monoclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (N-Term) antibody
SMAD2 Reactivity: Human, Mouse, Rat WB, IHC (p), ICC Host: Rabbit Polyclonal unconjugated

Application Notes

Optimal working dilutions should be determined experimentally by the investigator.

Comment

Antigen size: 467 AA

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freeze-thaw cycles.

Storage

-20 °C

Storage Comment

For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Target

SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Alternative Name

SMAD2 (SMAD2 Products)

Synonyms

JV18 antibody, JV18-1 antibody, MADH2 antibody, MADR2 antibody, hMAD-2 antibody, hSMAD2 antibody, jv18 antibody, Xmad2 antibody, madh2 antibody, madr2 antibody, XSmad2 antibody, hmad-2 antibody, hsmad2 antibody, jv18-1 antibody, smad-2 antibody, fj43c06 antibody, wu:fj43c06 antibody, 7120426M23Rik antibody, Madh2 antibody, Madr2 antibody, Smad-2 antibody, mMad2 antibody, MAD homolog 2 antibody, Mothers against DPP homolog 2 antibody, SMAD 2 antibody, Smad2 antibody, SMAD family member 2 antibody, SMAD family member 2-Z antibody, SMAD family member 2 S homeolog antibody, SMAD2 antibody, SMAD2Z antibody, smad2 antibody, smad2.S antibody, Smad2 antibody

Background

SMAD2 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. SMAD2 mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. SMAD2 is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, SMAD2 is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin.The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the same protein have been observed.
Alias Symbols: JV18, MADH2, MADR2, JV18-1, hMAD-2, hSMAD2
Protein Interaction Partner: HDAC2, UBC, SMAD4, SKIL, ANP32B, TUBA1B, SYT1, SOD1, ANP32E, PTMS, NEFM, JUN, ATP5I, RHOA, OTUB1, ZFYVE9, PPARG, TP53, TGFBR1, TRIM33, HCVgp1, TSC2, FHL3, FHL2, FHL1, CSNK1D, KLF5, SH2D2A, SKI, LCK, IRF7, IRF3, RNF111, LEMD3, PIK3CA, ST13, ST5, SRI, SPTBN
Protein Size: 467

Molecular Weight

51 kDa

Gene ID

4087

NCBI Accession

NM_005901, NP_005892

UniProt

Q15796

Pathways

Cell Division Cycle, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus