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PDK4 antibody (C-Term)

Name: PDK4 antibody (C-Term)
SKU: ABIN391039
Price: 429 USD
Availability: InStock

PDK4 Reactivity: Human, Mouse WB, IF, IHC (p) Host: Rabbit Polyclonal RB03041 unconjugated

Anti-PDK4 Antibody (C-term) at 1:2000 dilution + human heart lysate Lysates/proteins at 20 μg per lane.

IHC (p)

All lanes : Anti-PDK4 Antibody (C-term) at 1:2000 dilution Lane 1: human heart lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 μg per lane.

Fluorescent confocal image of HeLa cells stained with PDK4 (C-term) antibody.

All lanes : Anti-PDK4 Antibody at 1:2000 dilution Lane 1: human heart lysates Lane 2: mouse skeletal muscle lysates Lysates/proteins at 20 μg per lane.

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PDK4 Antibody (C-term) (ABIN391039 and ABIN2841206) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

IHC (p)

6 images

(24 references)

Catalog No. ABIN391039

Datasheet as PDF

Target See all PDK4 Antibodies

PDK4 (Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4))
Binding Specificity
15

14

8

8

7

6

5

5

5

5

2

1

1

1

1

1

1

1

1

1
AA 382-410, C-Term
Reactivity
72

46

9

2

2

2

2

1

1

1

1
Human, Mouse
Host
71

13
Rabbit
Compatible Secondaries
Clonality
75

11
Polyclonal
Conjugate
44

9

6

6

5

5

1

1

1

1

1

1

1

1

1

1

1
This PDK4 antibody is un-conjugated
Application
76

50

28

13

13

9

7

3

3

2

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen

This PDK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 382-410 amino acids from the C-terminal region of human PDK4.

Clone

RB03041

Isotype

Ig Fraction
anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (AA 117-387) antibody
PDK4 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (AA 382-410) antibody
PDK4 Reactivity: Human, Mouse WB, ELISA, IHC, IF, mIHC Host: Rabbit Polyclonal unconjugated

anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (C-Term) antibody
PDK4 Reactivity: Human, Mouse WB, ELISA, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (AA 102-411) antibody
PDK4 Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (AA 83-111) antibody
PDK4 Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (AA 83-111) antibody
PDK4 Reactivity: Human WB, IF, IHC (p) Host: Rabbit Polyclonal RB15302 unconjugated

anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (AA 1-411) antibody
PDK4 Reactivity: Human ELISA Host: Mouse Monoclonal 1A10 unconjugated

anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (N-Term) antibody
PDK4 Reactivity: Human, Mouse, Rat, Cow, Horse, Pig, Rabbit, Dog, Guinea Pig WB, IHC Host: Rabbit Polyclonal unconjugated

anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (AA 143-333) antibody
PDK4 Reactivity: Human ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (C-Term) antibody
PDK4 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

Application Notes

IF: 1:100. WB: 1:2000. WB: 1:2000. WB: 1:2000. IHC-P: 1:10~50. IHC-P: 1:25

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid freeze-thaw cycles.

Storage

4 °C,-20 °C

Storage Comment

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Expiry Date

6 months
Cole, Abd Jamil, Heather, Murray, Sutton, Slingo, Sebag-Montefiore, Tan, Aksentijević, Gildea, Stuckey, Yeoh, Carr, Evans, Aasum, Schofield, Ratcliffe, Neubauer, Robbins, Clarke: "On the pivotal role of PPARα in adaptation of the heart to hypoxia and why fat in the diet increases hypoxic injury." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 30, Issue 8, pp. 2684-97, (2017) (PubMed).

Zhang, Wu, Choiniere, Yang, Huang, Bennett, Wang: "Arsenic silences hepatic PDK4 expression through activation of histone H3K9 methylatransferase G9a." in: Toxicology and applied pharmacology, Vol. 304, pp. 42-7, (2017) (PubMed).

Zhang, Zhang, Geng, Yi, Huo, Talmon, Kim, Wang, Wang: "Transforming Growth Factor β Mediates Drug Resistance by Regulating the Expression of Pyruvate Dehydrogenase Kinase 4 in Colorectal Cancer." in: The Journal of biological chemistry, Vol. 291, Issue 33, pp. 17405-16, (2017) (PubMed).

Li, Li, Hu, Lei, Xiong, Ji, Yin, Wei, Hu: "The microRNA-182-PDK4 axis regulates lung tumorigenesis by modulating pyruvate dehydrogenase and lipogenesis." in: Oncogene, Vol. 36, Issue 7, pp. 989-998, (2017) (PubMed).

Jiang, de Castro Barbosa, Massart, Deshmukh, Löfgren, Duque-Guimaraes, Ozilgen, Osler, Chibalin, Zierath: "Diacylglycerol kinase-δ regulates AMPK signaling, lipid metabolism, and skeletal muscle energetics." in: American journal of physiology. Endocrinology and metabolism, Vol. 310, Issue 1, pp. E51-60, (2016) (PubMed).

Mazar, Qi, Lee, Marchica, Govindarajan, Shelley, Li, Ray, Perera: "MicroRNA 211 Functions as a Metabolic Switch in Human Melanoma Cells." in: Molecular and cellular biology, Vol. 36, Issue 7, pp. 1090-108, (2016) (PubMed).

Walter, Thomalla, Bruhn, Fagan, Zehowski, Yee, Skildum: "Altered regulation of PDK4 expression promotes antiestrogen resistance in human breast cancer cells." in: SpringerPlus, Vol. 4, pp. 689, (2015) (PubMed).

Kamarajugadda, Stemboroski, Cai, Simpson, Nayak, Tan, Lu: "Glucose oxidation modulates anoikis and tumor metastasis." in: Molecular and cellular biology, Vol. 32, Issue 10, pp. 1893-907, (2012) (PubMed).

Haldar, Jeyaraj, Anand, Zhu, Lu, Prosdocimo, Eapen, Kawanami, Okutsu, Brotto, Fujioka, Kerner, Rosca, McGuinness, Snow, Russell, Gerber, Bai, Yan, Nosek, Brotto, Hoppel, Jain: "Kruppel-like factor 15 regulates skeletal muscle lipid flux and exercise adaptation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 17, pp. 6739-44, (2012) (PubMed).

Williams, Wan, Frier, Bell, Field, Wright: "Dietary supplementation with vitamin E and C attenuates dexamethasone-induced glucose intolerance in rats." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 302, Issue 1, pp. R49-58, (2011) (PubMed).

Long, Kostovski, Boon, Hjeltnes, Krook, Widegren: "Differential expression of metabolic genes essential for glucose and lipid metabolism in skeletal muscle from spinal cord injured subjects." in: Journal of applied physiology (Bethesda, Md. : 1985), Vol. 110, Issue 5, pp. 1204-10, (2011) (PubMed).

Phillips, Aponte, Covian, Balaban: "Intrinsic protein kinase activity in mitochondrial oxidative phosphorylation complexes." in: Biochemistry, Vol. 50, Issue 13, pp. 2515-29, (2011) (PubMed).

Dunford, Herbst, Jeoung, Gittings, Inglis, Vandenboom, LeBlanc, Harris, Peters: "PDH activation during in vitro muscle contractions in PDH kinase 2 knockout mice: effect of PDH kinase 1 compensation." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 300, Issue 6, pp. R1487-93, (2011) (PubMed).

Attia, Connnaughton, Boone, Wang, Elam, Ness, Cook, Park: "Regulation of pyruvate dehydrogenase kinase 4 (PDK4) by thyroid hormone: role of the peroxisome proliferator-activated receptor gamma coactivator (PGC-1 alpha)." in: The Journal of biological chemistry, Vol. 285, Issue 4, pp. 2375-85, (2010) (PubMed).

Dateki, Kunitomo, Yoshioka, Yanai, Nakasono, Negishi: "Adaptive gene regulation of pyruvate dehydrogenase kinase isoenzyme 4 in hepatotoxic chemical-induced liver injury and its stimulatory potential for DNA repair and cell proliferation." in: Journal of receptor and signal transduction research, Vol. 31, Issue 1, pp. 85-95, (2010) (PubMed).

Zhao, Okuyama, Hashimoto, Tagawa, Jomori, Yang: "Bezafibrate induces myotoxicity in human rhabdomyosarcoma cells via peroxisome proliferator-activated receptor alpha signaling." in: Toxicology in vitro : an international journal published in association with BIBRA, Vol. 24, Issue 1, pp. 154-9, (2010) (PubMed).

Basu, Oudit, Wang, Zhang, Ussher, Lopaschuk, Kassiri: "Type 1 diabetic cardiomyopathy in the Akita (Ins2WT/C96Y) mouse model is characterized by lipotoxicity and diastolic dysfunction with preserved systolic function." in: American journal of physiology. Heart and circulatory physiology, Vol. 297, Issue 6, pp. H2096-108, (2009) (PubMed).

Hsieh, Das, Sambandam, Zhang, Nahlé: "Regulation of the PDK4 isozyme by the Rb-E2F1 complex." in: The Journal of biological chemistry, Vol. 283, Issue 41, pp. 27410-7, (2008) (PubMed).

Nahlé, Hsieh, Pietka, Coburn, Grimaldi, Zhang, Das, Abumrad: "CD36-dependent regulation of muscle FoxO1 and PDK4 in the PPAR delta/beta-mediated adaptation to metabolic stress." in: The Journal of biological chemistry, Vol. 283, Issue 21, pp. 14317-26, (2008) (PubMed).

Long, Glund, Garcia-Roves, Zierath: "Calcineurin regulates skeletal muscle metabolism via coordinated changes in gene expression." in: The Journal of biological chemistry, Vol. 282, Issue 3, pp. 1607-14, (2007) (PubMed).

show more references
Target

PDK4 (Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4))

Alternative Name

PDK4 (PDK4 Products)

Synonyms

AV005916 antibody, pyruvate dehydrogenase kinase 4 antibody, pyruvate dehydrogenase kinase, isoenzyme 4 antibody, pdk4 antibody, PDK4 antibody, Pdk4 antibody

Background

PDK4 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.

Molecular Weight

46469

Gene ID

5166

NCBI Accession

NP_002603

UniProt

Q16654

Pathways

PI3K-Akt Signaling, Hedgehog Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect