PPP2R1A antibody (Center)
(1 reference)-
- Target See all PPP2R1A Antibodies
- PPP2R1A (Protein Phosphatase 2, Regulatory Subunit A, alpha (PPP2R1A))
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Binding Specificity
- Center
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Reactivity
- Human, Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PPP2R1A antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
- Cross-Reactivity
- Rat (Rattus), Pig (Porcine), Rhesus Monkey
- Cross-Reactivity (Details)
- Rat (100 %), Pig (100 %), Rhesus Monkey (100 %)
- Characteristics
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Rabbit Polyclonal antibody to PPP2R1A (serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform)
PPP2R1A antibody - Purification
- Purified by antigen-affinity chromatography.
- Immunogen
- Recombinant protein encompassing a sequence within the center region of human PPP2R1A. The exact sequence is proprietary.
- Isotype
- IgG
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anti-Protein Phosphatase 2, Regulatory Subunit A, alpha (PPP2R1A) (AA 1-589) antibody
PPP2R1A Reactivity: Human WB, ELISA, PLA Host: Mouse Monoclonal 4E6 unconjugated
anti-Protein Phosphatase 2, Regulatory Subunit A, alpha (PPP2R1A) (C-Term) antibodyPPP2R1A Reactivity: Human, Mouse, Rat, Monkey, Drosophila melanogaster WB, IF, FACS Host: Rabbit Polyclonal unconjugated
anti-Protein Phosphatase 2, Regulatory Subunit A, alpha (PPP2R1A) (AA 454-481), (C-Term) antibodyPPP2R1A Reactivity: Human WB Host: Rabbit Polyclonal RB41029 unconjugated
anti-Protein Phosphatase 2, Regulatory Subunit A, alpha (PPP2R1A) antibodyPPP2R1A Reactivity: Human, Mouse WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated
anti-Protein Phosphatase 2, Regulatory Subunit A, alpha (PPP2R1A) (C-Term) antibodyVerified PPP2R1A Reactivity: Human WB, ELISA Host: Goat Polyclonal unconjugated
anti-Protein Phosphatase 2, Regulatory Subunit A, alpha (PPP2R1A) antibodyPPP2R1A Reactivity: Human WB, ELISA, IHC, IHC (p) Host: Mouse Monoclonal 4E6 unconjugated
anti-Protein Phosphatase 2, Regulatory Subunit A, alpha (PPP2R1A) (AA 107-156) antibodyPPP2R1A Reactivity: Human, Mouse, Rat, Cow, Horse, Guinea Pig, Pig, Monkey, Xenopus laevis, Zebrafish (Danio rerio) WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Protein Phosphatase 2, Regulatory Subunit A, alpha (PPP2R1A) (AA 1-270) antibodyPPP2R1A Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated
anti-Protein Phosphatase 2, Regulatory Subunit A, alpha (PPP2R1A) antibodyPPP2R1A Reactivity: Human, Mouse, Rat IF Host: Rabbit Polyclonal unconjugated
anti-Protein Phosphatase 2, Regulatory Subunit A, alpha (PPP2R1A) (AA 1-589) antibodyPPP2R1A Reactivity: Human WB Host: Rabbit Polyclonal unconjugated
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- Application Notes
- Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation Assay-dependent dilution Western blot 1:1000-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* ImmunoprecipitationAssay-dependent dilution Western blot1:1000-1:10000*
- Comment
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Positive Control: HeLa , NIH-3T3 , JC , BCL-1
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.96 mg/mL
- Buffer
- 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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: "PP2A Deficiency Enhances Carcinogenesis of Lgr5+ Intestinal Stem Cells Both in Organoids and In Vivo." in: Cells, Vol. 9, Issue 1, (2020) (PubMed).
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: "PP2A Deficiency Enhances Carcinogenesis of Lgr5+ Intestinal Stem Cells Both in Organoids and In Vivo." in: Cells, Vol. 9, Issue 1, (2020) (PubMed).
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- Target
- PPP2R1A (Protein Phosphatase 2, Regulatory Subunit A, alpha (PPP2R1A))
- Alternative Name
- PPP2R1A (PPP2R1A Products)
- Synonyms
- PP2A-Aalpha antibody, PP2AAALPHA antibody, PR65A antibody, PR65 antibody, pp2a-aalpha antibody, pp2aaalpha antibody, ppp2r1a antibody, pr65a antibody, wu:fa02h04 antibody, zgc:56296 antibody, 6330556D22Rik antibody, PP2A antibody, ppp2r1a-a antibody, ppp2r1a-b antibody, protein phosphatase 2 scaffold subunit Aalpha antibody, protein phosphatase 2 scaffold subunit A alpha antibody, protein phosphatase 2 regulatory subunit A, alpha L homeolog antibody, protein phosphatase 2 regulatory subunit A, alpha antibody, protein phosphatase 2, regulatory subunit A, beta a antibody, serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform antibody, protein phosphatase 2, regulatory subunit A, alpha isoform antibody, protein phosphatase 2, regulatory subunit A, alpha antibody, protein phosphatase 2 regulatory subunit A, alpha S homeolog antibody, PPP2R1A antibody, Ppp2r1a antibody, ppp2r1a.L antibody, ppp2r1a antibody, ppp2r1ba antibody, LOC583227 antibody, ppp2r1a.S antibody
- Background
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This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A.
- Molecular Weight
- 65 kDa
- Gene ID
- 5518
- Pathways
- PI3K-Akt Signaling, Mitotic G1-G1/S Phases, M Phase, Hepatitis C, Toll-Like Receptors Cascades
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