PHKA1 antibody (C-Term)
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- Target See all PHKA1 Antibodies
- PHKA1 (phosphorylase Kinase, alpha 1 (Muscle) (PHKA1))
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Binding Specificity
- C-Term
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PHKA1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
- Cross-Reactivity
- Mouse (Murine), Rat (Rattus)
- Cross-Reactivity (Details)
- Mouse (96 %), Rat (95 %)
- Characteristics
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Rabbit Polyclonal antibody to PHKA1 (phosphorylase kinase, alpha 1 (muscle))
PHKA1 antibody [C2C3], C-term - Purification
- Purified by antigen-affinity chromatography.
- Immunogen
- Recombinant protein encompassing a sequence within the C-terminus region of human PHKA1. The exact sequence is proprietary.
- Isotype
- IgG
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anti-phosphorylase Kinase, alpha 1 (Muscle) (PHKA1) (AA 631-730) antibody
PHKA1 Reactivity: Human WB, ELISA Host: Mouse Polyclonal unconjugated
anti-phosphorylase Kinase, alpha 1 (Muscle) (PHKA1) (AA 555-688), (Regulatory Subunit A) antibodyPHKA1 Reactivity: Human ELISA, IHC Host: Rabbit Polyclonal unconjugated
anti-phosphorylase Kinase, alpha 1 (Muscle) (PHKA1) (AA 600-820) antibodyPHKA1 Reactivity: Human WB Host: Rabbit Polyclonal unconjugated
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- Application Notes
- Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
- Comment
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Positive Control: 293T , A431 , H1299 , HeLa
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- Target
- PHKA1 (phosphorylase Kinase, alpha 1 (Muscle) (PHKA1))
- Alternative Name
- PHKA1 (PHKA1 Products)
- Synonyms
- PHKA1 antibody, wu:fl37h01 antibody, 5330411D17 antibody, 9830108K24Rik antibody, Phka antibody, Pcyt1b antibody, PHKA antibody, phosphorylase kinase regulatory subunit alpha 1 antibody, phosphorylase kinase, alpha 1a (muscle) antibody, phosphorylase kinase, alpha 1 (muscle) antibody, phosphorylase kinase alpha 1 antibody, PHKA1 antibody, phka1a antibody, phka1 antibody, Phka1 antibody
- Background
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The PHKA1 gene encodes the alpha subunit of muscle phosphorylase kinase (EC 2.7.1.38), a key regulatory enzyme of glycogen metabolism. Phosphorylase kinase consists of 4 copies of an alpha-beta-gamma-delta tetramer. The alpha, beta (PHKB, MIM 172490), and gamma (PHKG1, MIM 172470 and PHKG2, MIM 172471) subunits have several isoforms, the delta subunit is calmodulin (CALM1, MIM 114180). PHKA2 (MIM 306000) encodes the alpha subunit of liver-specific phosphorylase kinase and is also located on the X chromosome.[supplied by OMIM]
Cellular Localization: Cell membrane, Lipid-anchor, Cytoplasmic side - Molecular Weight
- 137 kDa
- Gene ID
- 5255
- Pathways
- Cellular Glucan Metabolic Process
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