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POU2AF1 antibody (AA 148-255)

POU2AF1 Reactivity: Human WB, IHC, FACS, IF, StM Host: Mouse Monoclonal BOB1-2424 unconjugated
Catalog No. ABIN6940356
  • Target See all POU2AF1 Antibodies
    POU2AF1 (POU Class 2 Associating Factor 1 (POU2AF1))
    Binding Specificity
    • 15
    • 15
    • 14
    • 8
    • 6
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    AA 148-255
    Reactivity
    • 59
    • 20
    • 6
    Human
    Host
    • 66
    • 8
    • 2
    Mouse
    Clonality
    • 64
    • 12
    Monoclonal
    Conjugate
    • 34
    • 6
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This POU2AF1 antibody is un-conjugated
    Application
    • 51
    • 32
    • 26
    • 26
    • 23
    • 18
    • 15
    • 6
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Staining Methods (StM)
    Purification
    Purified by Protein A/G
    Immunogen
    Recombinant fragment (around aa 148-255) of human BOB1 (POU2AF1) protein (exact sequence is proprietary)
    Clone
    BOB1-2424
    Isotype
    IgG2b kappa
  • Application Notes

    Positive Control: Raji, Ramos cells. Spleen or Tonsil.

    Known Application: Flow Cytometry (1-2 μg/mL), Immunofluorescence (1-2 μg/mL),Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-80 °C
    Storage Comment
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Expiry Date
    24 months
  • Target
    POU2AF1 (POU Class 2 Associating Factor 1 (POU2AF1))
    Alternative Name
    POU2AF1 (POU2AF1 Products)
    Synonyms
    BOB1 antibody, OBF-1 antibody, OBF1 antibody, OCAB antibody, BOB-1 antibody, BOB.1 antibody, Bob-1 antibody, Bob1 antibody, OBF.1 antibody, OCA-B antibody, POU class 2 associating factor 1 antibody, POU domain, class 2, associating factor 1 antibody, POU2AF1 antibody, Pou2af1 antibody
    Background
    BOB.1 expression in a variety of established B-cell lines, representing different stages of B-cell development, has suggested a constitutive, B-cell-specific expression pattern. LP cells in nodular lymphocyte predominant Hodgkin lymphoma, because they are germinal center-derived, are consistently immuno-positive for BOB.1. Conversely, only some cases of classical Hodgkin lymphoma show BOB.1 immuno-reactivity within the Hodgkin and Reed-Sternberg cells.Expression of BOB.1 has been reported in follicular center cell lymphoma, diffuse large B-cell lymphoma and some cases of acute myeloid leukemia.B-CLL, marginal zone lymphoma, and mantle cell lymphoma may show weak to moderate immunoreactivity.
    Molecular Weight
    35kDa
    Gene ID
    5450
    UniProt
    Q16633
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