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ATRX antibody (C-Term)

Name: ATRX antibody (C-Term)
Brand: antibodies-online
SKU: ABIN2855005
Price: 609.18 USD
Availability: InStock

ATRX Reactivity: Human, Mouse WB, IF, IHC (p), ICC, IP Host: Rabbit Polyclonal unconjugated

IHC-P Image Rad54 antibody [C3], C-term detects Rad54 protein at nucleus on human lung carcinoma by immunohistochemical analysis.

IHC

WB Image ATRX antibody detects ATRX protein by western blot analysis.

IP Image Immunoprecipitation of ATRX protein from 293T whole cell extracts using 5 μg of ATRX antibody [C3], C-term, Western blot analysis was performed using ATRX antibody [C3], C-term, EasyBlot anti-Rabbit IgG was used as a secondary reagent.

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ATRX, antibody at 1:200 dilution.

IHC-P Image Rad54 antibody [C3], C-term detects Rad54 protein at nucleus on human endometrial carcinoma by immunohistochemical analysis.

IHC

IHC-P Image Rad54 antibody [C3], C-term detects Rad54 protein at nucleus on human breast carcinoma by immunohistochemical analysis.

IHC

7 images

Catalog No. ABIN2855005

Datasheet as PDF

Target See all ATRX Antibodies

ATRX (helicase 2, X-linked (ATRX))
Binding Specificity
12

6

6

3

3

2

1

1

1

1

1

1

1

1

1

1

1

1

1
C-Term
Reactivity
61

16

3

3

2

2
Human, Mouse
Host
40

23

1
Rabbit
Compatible Secondaries
Clonality
37

27
Polyclonal
Conjugate
39

4

4

3

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This ATRX antibody is un-conjugated
Application
38

29

20

18

16

11

10

4

3

2

2

2

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Cross-Reactivity

Chimpanzee

Cross-Reactivity (Details)

Chimpanzee (100 %)

Characteristics

Rabbit Polyclonal antibody to ATRX (alpha thalassemia/mental retardation syndrome X-linked (RAD54 homolog, S. cerevisiae))
ATRX antibody [C3], C-term

Purification

Purified by antigen-affinity chromatography.

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ATRX. The exact sequence is proprietary.

Isotype

IgG
anti-helicase 2, X-linked (ATRX) (AA 2273-2413) antibody
ATRX Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-helicase 2, X-linked (ATRX) (AA 2161-2443) antibody
ATRX Reactivity: Human ELISA, IF, IHC Host: Rabbit Polyclonal unconjugated

anti-helicase 2, X-linked (ATRX) (AA 2311-2492) antibody
ATRX Reactivity: Human ELISA, WB, IHC, FACS, ICC, Neut Host: Mouse Monoclonal 8B2H9 unconjugated

anti-helicase 2, X-linked (ATRX) antibody
ATRX Reactivity: Human WB, IF, IHC (p) Host: Mouse Monoclonal CL0537 unconjugated

anti-helicase 2, X-linked (ATRX) (N-Term) antibody
ATRX Reactivity: Human, Mouse ELISA, WB, IF, IHC, ICC Host: Rabbit Polyclonal unconjugated

anti-helicase 2, X-linked (ATRX) (AA 634-663), (C-Term) antibody
ATRX Reactivity: Human WB, FACS Host: Rabbit Polyclonal RB20635 unconjugated

anti-helicase 2, X-linked (ATRX) antibody
ATRX Reactivity: Human, Mouse ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-helicase 2, X-linked (ATRX) (AA 2311-2410) antibody
ATRX Reactivity: Human ELISA, WB, IF Host: Mouse Monoclonal 5B3 unconjugated

anti-helicase 2, X-linked (ATRX) antibody
ATRX Reactivity: Human IHC, ISt, StM Host: Rabbit Monoclonal ATRX-2900R unconjugated Recombinant Antibody

anti-helicase 2, X-linked (ATRX) antibody
ATRX Reactivity: Human IHC, StM Host: Mouse Monoclonal 39f unconjugated

Application Notes

Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*

Comment

Positive Control: 293T , A431 , HeLa , HepG2 , NIH3T3

Restrictions

For Research Use only
Format

Liquid

Concentration

2.67 mg/mL

Buffer

1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Target

ATRX (helicase 2, X-linked (ATRX))

Alternative Name

ATRX (ATRX Products)

Background

The protein encoded by this gene contains an ATPase/helicase domain, and thus it belongs to the SWI/SNF family of chromatin remodeling proteins. The mutations of this gene are associated with an X-linked mental retardation (XLMR) syndrome most often accompanied by alpha-thalassemia (ATRX) syndrome. These mutations have been shown to cause diverse changes in the pattern of DNA methylation, which may provide a link between chromatin remodeling, DNA methylation, and gene expression in developmental processes. This protein is found to undergo cell cycle-dependent phosphorylation, which regulates its nuclear matrix and chromatin association, and suggests its involvement in the gene regulation at interphase and chromosomal segregation in mitosis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported.

Molecular Weight

283 kDa

Gene ID

546