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PPP2CA antibody (C-Term)

Name: PPP2CA antibody (C-Term)
Brand: antibodies-online
SKU: ABIN2856333
Price: 609.18 USD
Availability: InStock

PPP2CA Reactivity: Human, Mouse, Rat WB, IF, IHC (p), IP, ICC Host: Rabbit Polyclonal unconjugated

WB Image Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE antibody diluted at 1:10000

ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PP2A alpha, antibody (Green) at 1:500 dilution.

IP Image Immunoprecipitation of PP2A alpha protein from 293T whole cell extracts using 5 μg of PP2A alpha antibody, Western blot analysis was performed using PP2A alpha antibody, EasyBlot anti-Rabbit IgG was used as a secondary reagent.

IHC-P Image Immunohistochemical analysis of paraffin-embedded A549 xenograft, using PPP2CA , antibody at 1:100 dilution.

IHC

WB Image Sample (30 ug of whole cell lysate) A: 293T B: Molt-4 , 10% SDS PAGE antibody diluted at 1:1500

Effects of PMC on the regulation of PP2A in LPS/IFN-γ-stimulated VSMCs.

Role of ROS in the inhibitory effects of PMC in LPS/IFN-γ-stimulated VSMCs.

8 images

Catalog No. ABIN2856333

Datasheet as PDF

Target See all PPP2CA Antibodies

PPP2CA (Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA))
Binding Specificity
16

14

13

8

7

6

6

3

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
C-Term
Reactivity
67

56

46

13

10

7

7

7

6

5

5

3

3

3

1

1

1

1
Human, Mouse, Rat
Host
75

8

4

2

1
Rabbit
Compatible Secondaries
Clonality
75

15
Polyclonal
Conjugate
56

4

4

4

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1
This PPP2CA antibody is un-conjugated
Application
75

30

18

16

15

14

13

13

12

4

4

2

2

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Cross-Reactivity

Rat (Rattus), Zebrafish (Danio rerio), Xenopus laevis, Xenopus tropicalis, Dog (Canine), Pig (Porcine), Rabbit, Chicken, Cow (Bovine)

Cross-Reactivity (Details)

Rat (100 %), Zebrafish (100 %), Xenopus laevis (100 %), Xenopus Tropicalis (100 %), Dog (100 %), Pig (100 %), Rabbit (100 %), Chicken (100 %), Bovine (100 %)

Characteristics

Rabbit Polyclonal antibody to PP2A alpha (protein phosphatase 2, catalytic subunit, alpha isozyme)
PP2A alpha antibody

Purification

Purified by antigen-affinity chromatography.

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PP2A alpha. The exact sequence is proprietary.

Isotype

IgG
anti-Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) (AA 1-309), (Catalytic Subunit alpha) antibody
PPP2CA Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) (Thr278) antibody
PPP2CA Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) antibody
PPP2CA Reactivity: Human, Mouse, Rat WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) (N-Term) antibody
PPP2CA Reactivity: Human, Mouse, Rat WB, IHC (p), ICC Host: Rabbit Polyclonal unconjugated

anti-Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) (AA 1-309) antibody
PPP2CA Reactivity: Human, Mouse, Rat WB, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) (N-Term) antibody
PPP2CA Reactivity: Human WB, ELISA Host: Rabbit Polyclonal unconjugated

anti-Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) (C-Term) antibody
PPP2CA Reactivity: Human WB, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) (pTyr307) antibody
PPP2CA Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) (AA 274-304), (C-Term) antibody
PPP2CA Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB6000 unconjugated

anti-Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) (AA 1-30), (N-Term) antibody
PPP2CA Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB05998 unconjugated

Application Notes

Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:1000-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:1000-1:10000*

Comment

Positive Control: 293T , A431 , H1299 , HeLaS3 , Molt-4 , Raji , mouse brain

Restrictions

For Research Use only
Format

Liquid

Concentration

0.479 mg/mL

Buffer

1XPBS, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative

Thimerosal (Merthiolate)

Precaution of Use

This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Target

PPP2CA (Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA))

Alternative Name

PP2A alpha (PPP2CA Products)

Synonyms

PP2Ac antibody, PP2CA antibody, PP2Calpha antibody, RP-C antibody, PP2A antibody, R75353 antibody, Pp2a1 antibody, pp2ac antibody, zgc:110641 antibody, protein phosphatase 2 catalytic subunit alpha antibody, protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform antibody, protein phosphatase 2, catalytic subunit, alpha isozyme antibody, protein phosphatase 2 catalytic subunit alpha L homeolog antibody, protein phosphatase 2, catalytic subunit, beta isozyme antibody, serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform antibody, PPP2CA antibody, Ppp2ca antibody, ppp2ca.L antibody, ppp2cb antibody, LOC471633 antibody, ppp2ca antibody

Background

This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit.

Cellular Localization: Cytoplasm , Nucleus , Centromere , Spindle

Molecular Weight

36 kDa

Gene ID

5515

Pathways

PI3K-Akt Signaling, Mitotic G1-G1/S Phases, Hepatitis C, Toll-Like Receptors Cascades