RFWD3 antibody (N-Term)
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- Target See all RFWD3 Antibodies
- RFWD3 (Ring Finger and WD Repeat Domain 3 (RFWD3))
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Binding Specificity
- N-Term
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This RFWD3 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
- Specificity
- RFWD3 Antibody detects endogenous levels of total RFWD3.
- Purification
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- Immunogen
- A synthesized peptide derived from human RFWD3, corresponding to a region within N-terminal amino acids.
- Isotype
- IgG
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anti-Ring Finger and WD Repeat Domain 3 (RFWD3) (Internal Region) antibody
RFWD3 Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated
anti-Ring Finger and WD Repeat Domain 3 (RFWD3) (AA 651-749) antibodyRFWD3 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 6B4 unconjugated
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- Application Notes
- WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20 °C. Stable for 12 months from date of receipt.
- Expiry Date
- 12 months
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- Target
- RFWD3 (Ring Finger and WD Repeat Domain 3 (RFWD3))
- Alternative Name
- RFWD3 (RFWD3 Products)
- Synonyms
- RGD1305243 antibody, RFWD3 antibody, RNF201 antibody, BC027246 antibody, ring finger and WD repeat domain 3 antibody, Rfwd3 antibody, RFWD3 antibody
- Background
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Description: E3 ubiquitin-protein ligase required for the repair of DNA interstrand cross-links (ICL) in response to DNA damage (PubMed:21504906, PubMed:21558276, PubMed:26474068, PubMed:28575657, PubMed:28575658). Plays a key role in RPA-mediated DNA damage signaling and repair (PubMed:21504906, PubMed:21558276, PubMed:26474068, PubMed:28575657, PubMed:28575658). Acts by mediating ubiquitination of the RPA complex (RPA1, RPA2 and RPA3 subunits) and RAD51 at stalled replication forks, leading to remove them from DNA damage sites and promote homologous recombination (PubMed:26474068, PubMed:28575657, PubMed:28575658). Also mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint (PubMed:20173098). May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome (PubMed:20173098). In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53 (PubMed:20173098).
Gene: RFWD3
- Molecular Weight
- 85 kDa
- Gene ID
- 55159
- UniProt
- Q6PCD5
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