PRKACA antibody (Center)
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- Target See all PRKACA Antibodies
- PRKACA (Protein Kinase A, alpha (PRKACA))
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Binding Specificity
- Center
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Reactivity
- Human, Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PRKACA antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)
- Cross-Reactivity
- Rat (Rattus), Dog (Canine), Sheep (Ovine), Cow (Bovine)
- Cross-Reactivity (Details)
- Rat (100 %), Dog (100 %), Sheep (100 %), Bovine (100 %)
- Characteristics
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Rabbit Polyclonal antibody to PKA C alpha (protein kinase, cAMP-dependent, catalytic, alpha)
PKA C alpha antibody [N2C3] - Purification
- Purified by antigen-affinity chromatography.
- Immunogen
- Recombinant protein encompassing a sequence within the center region of human PKA C alpha. The exact sequence is proprietary.
- Isotype
- IgG
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anti-Protein Kinase A, alpha (PRKACA) (N-Term) antibody
PRKACA Reactivity: Human, Mouse WB, ELISA, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Protein Kinase A, alpha (PRKACA) (AA 233-351), (Catalytic Subunit alpha) antibodyPRKACA Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated
anti-Protein Kinase A, alpha (PRKACA) (Internal Region) antibodyPRKACA Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Protein Kinase A, alpha (PRKACA) (pThr197) antibodyPRKACA Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Protein Kinase A, alpha (PRKACA) (AA 67-94), (N-Term) antibodyPRKACA Reactivity: Human WB, IHC (p), FACS Host: Rabbit Polyclonal RB20762 unconjugated
anti-Protein Kinase A, alpha (PRKACA) (AA 1-120) antibodyPRKACA Reactivity: Human WB, ELISA Host: Mouse Monoclonal 7H3A4 unconjugated
anti-Protein Kinase A, alpha (PRKACA) (pThr197) antibodyPRKACA Reactivity: Human, Mouse, Rat WB, IHC (p), IF Host: Rabbit Polyclonal unconjugated
anti-Protein Kinase A, alpha (PRKACA) (AA 1-120) antibodyPRKACA Reactivity: Human WB, ELISA, PLA Host: Mouse Monoclonal 1D7 unconjugated
anti-Protein Kinase A, alpha (PRKACA) (AA 1-30), (N-Term), (Regulatory Subunit 1) antibodyPRKACA Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB3247 unconjugated
anti-Protein Kinase A, alpha (PRKACA) (AA 1-120) antibodyPRKACA Reactivity: Human WB, ELISA Host: Mouse Polyclonal unconjugated
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- Application Notes
- Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
- Comment
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Positive Control: HeLa , Jurkat , NT2D1 , mouse brain
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.38 mg/mL
- Buffer
- 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- Target
- PRKACA (Protein Kinase A, alpha (PRKACA))
- Alternative Name
- PKA C alpha (PRKACA Products)
- Synonyms
- PKACA antibody, Calpha antibody, Cs-PKA antibody, PKCA1 antibody, PKA antibody, PKCD antibody, Pkaca antibody, zgc:158799 antibody, protein kinase cAMP-activated catalytic subunit alpha antibody, protein kinase, cAMP dependent, catalytic, alpha antibody, cAMP-dependent protein kinase catalytic subunit antibody, protein kinase, cAMP-dependent, catalytic, alpha, genome duplicate a antibody, PRKACA antibody, Prkaca antibody, kin-1 antibody, prkacaa antibody
- Background
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CAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Cellular Localization: Cytoplasm (By similarity) , Nucleus (By similarity) - Molecular Weight
- 41 kDa
- Gene ID
- 5566
- Pathways
- NF-kappaB Signaling, Hedgehog Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, M Phase, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, Lipid Metabolism, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
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