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PKC iota antibody (AA 375-596)

PRKCI Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN7444109
  • Target See all PKC iota (PRKCI) Antibodies
    PKC iota (PRKCI) (Protein Kinase C, iota (PRKCI))
    Binding Specificity
    • 15
    • 7
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 375-596
    Reactivity
    • 42
    • 35
    • 13
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Host
    • 38
    • 4
    Rabbit
    Clonality
    • 38
    • 4
    Polyclonal
    Conjugate
    • 16
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This PKC iota antibody is un-conjugated
    Application
    • 34
    • 17
    • 13
    • 13
    • 9
    • 6
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
    Purpose
    Polyclonal Antibody to Protein Kinase C Iota (PKCi)
    Specificity
    The antibody is a rabbit polyclonal antibody raised against PKCi. It has been selected for its ability to recognize PKCi in immunohistochemical staining and western blotting.
    Cross-Reactivity
    Mouse, Rat
    Purification
    Antigen-specific affinity chromatography followed by Protein A affinity chromatography
    Immunogen
    Recombinant Protein Kinase C Iota (PKCi) corresdonding to Ile375~Val596 (Accession # P41743)
    Isotype
    IgG
  • Application Notes

    Western blotting: 1-5 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
    Expiry Date
    24 months
  • Target
    PKC iota (PRKCI) (Protein Kinase C, iota (PRKCI))
    Alternative Name
    Protein Kinase C Iota (PRKCI Products)
    Synonyms
    DXS1179E antibody, PKCI antibody, nPKC-iota antibody, aPKC[l] antibody, hal antibody, has antibody, PRKCI antibody, PKC antibody, 2310021H13Rik antibody, AI427505 antibody, PKClambda antibody, Pkci antibody, Pkcl antibody, Prkcl antibody, aPKClambda antibody, mKIAA4165 antibody, protein kinase C iota antibody, protein kinase C, iota antibody, PRKCI antibody, prkci antibody, Prkci antibody
    Background
    PKC-I, PRKCI, PRKC-I, NPKC-Iota, Atypical protein kinase C-lambda/iota
    Pathways
    Neurotrophin Signaling Pathway, Cell-Cell Junction Organization, Tube Formation
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