PKC zeta antibody (C-Term)
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- Target See all PKC zeta (PRKCZ) Antibodies
- PKC zeta (PRKCZ) (Protein Kinase C, zeta (PRKCZ))
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Binding Specificity
- C-Term
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PKC zeta antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
- Specificity
- PKC zeta Antibody detects endogenous levels of total PKC zeta.
- Predicted Reactivity
- Pig,Zebrafish,Bovine,Horse,Rabbit,Dog,Chicken,Xenopus
- Purification
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- Immunogen
- A synthesized peptide derived from human PKC zeta, corresponding to a region within C-terminal amino acids.
- Isotype
- IgG
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anti-Protein Kinase C, zeta (PRKCZ) (pThr410) antibody
PRKCZ Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Protein Kinase C, zeta (PRKCZ) (AA 165-255) antibodyPRKCZ Reactivity: Human WB, ELISA, RNAi Host: Mouse Monoclonal 2D1 unconjugated
anti-Protein Kinase C, zeta (PRKCZ) (AA 453-592) antibodyPRKCZ Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated
anti-Protein Kinase C, zeta (PRKCZ) (pThr560) antibodyPRKCZ Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Protein Kinase C, zeta (PRKCZ) (AA 166-197), (N-Term) antibodyPRKCZ Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB01027 unconjugated
anti-Protein Kinase C, zeta (PRKCZ) (Cytoplasmic Domain) antibodyPRKCZ Reactivity: Human ELISA, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Protein Kinase C, zeta (PRKCZ) (pThr410) antibodyPRKCZ Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Protein Kinase C, zeta (PRKCZ) (pThr410) antibodyPRKCZ Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Protein Kinase C, zeta (PRKCZ) (Thr33) antibodyPRKCZ Reactivity: Human, Mouse, Rat, Monkey WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Protein Kinase C, zeta (PRKCZ) (N-Term) antibodyPRKCZ Reactivity: Human ELISA, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
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- Application Notes
- WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20 °C. Stable for 12 months from date of receipt.
- Expiry Date
- 12 months
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- Target
- PKC zeta (PRKCZ) (Protein Kinase C, zeta (PRKCZ))
- Alternative Name
- PRKCZ (PRKCZ Products)
- Background
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Description: Calcium- and diacylglycerol-independent serine/threonine-protein kinase that functions in phosphatidylinositol 3-kinase (PI3K) pathway and mitogen-activated protein (MAP) kinase cascade, and is involved in NF-kappa-B activation, mitogenic signaling, cell proliferation, cell polarity, inflammatory response and maintenance of long-term potentiation (LTP). Upon lipopolysaccharide (LPS) treatment in macrophages, or following mitogenic stimuli, functions downstream of PI3K to activate MAP2K1/MEK1-MAPK1/ERK2 signaling cascade independently of RAF1 activation. Required for insulin-dependent activation of AKT3, but may function as an adapter rather than a direct activator. Upon insulin treatment may act as a downstream effector of PI3K and contribute to the activation of translocation of the glucose transporter SLC2A4/GLUT4 and subsequent glucose transport in adipocytes. In EGF-induced cells, binds and activates MAP2K5/MEK5-MAPK7/ERK5 independently of its kinase activity and can activate JUN promoter through MEF2C. Through binding with SQSTM1/p62, functions in interleukin-1 signaling and activation of NF-kappa-B with the specific adapters RIPK1 and TRAF6. Participates in TNF-dependent transactivation of NF-kappa-B by phosphorylating and activating IKBKB kinase, which in turn leads to the degradation of NF-kappa-B inhibitors. In migrating astrocytes, forms a cytoplasmic complex with PARD6A and is recruited by CDC42 to function in the establishment of cell polarity along with the microtubule motor and dynein. In association with FEZ1, stimulates neuronal differentiation in PC12 cells. In the inflammatory response, is required for the T-helper 2 (Th2) differentiation process, including interleukin production, efficient activation of JAK1 and the subsequent phosphorylation and nuclear translocation of STAT6. May be involved in development of allergic airway inflammation (asthma), a process dependent on Th2 immune response. In the NF-kappa-B-mediated inflammatory response, can relieve SETD6-dependent repression of NF-kappa-B target genes by phosphorylating the RELA subunit at 'Ser-311'. Necessary and sufficient for LTP maintenance in hippocampal CA1 pyramidal cells. In vein endothelial cells treated with the oxidant peroxynitrite, phosphorylates STK11 leading to nuclear export of STK11, subsequent inhibition of PI3K/Akt signaling, and increased apoptosis. Phosphorylates VAMP2 in vitro (PubMed:17313651).
Gene: PRKCZ
- Molecular Weight
- 80kDa
- Gene ID
- 5590
- UniProt
- Q05513
- Pathways
- NF-kappaB Signaling, RTK Signaling, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling
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