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MEK2 antibody (AA 1-50)

Name: MEK2 antibody (AA 1-50)
SKU: ABIN726500
Price: 384.62 USD
Availability: InStock
Rating: 5 (1 reviews)

MAP2K2 Reactivity: Human, Mouse WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Western Blotting validation image for anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 1-50) antibody (ABIN726500)

Images provided by the Independent Validation Program, badge number 029748.

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti MEK2/MAPKK2 Polyclonal Antibody, Unconjugated (ABIN726500) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

IHC

HT-55 cells were stained with bs-0223R Rabbit Anti-MEK2 Polyclonal Antibody at 1:250.

IF (cc)

4 images

(1 validation)

Catalog No. ABIN726500

Datasheet as PDF

Target See all MEK2 (MAP2K2) Antibodies

MEK2 (MAP2K2) (Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2))
Binding Specificity
18

16

13

11

7

7

6

6

5

4

3

3

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 1-50
Reactivity
133

80

46

6

5

3

2

2

1
Human, Mouse
Host
108

24

1

1
Rabbit
Compatible Secondaries
Clonality
109

25
Polyclonal
Conjugate
70

11

11

10

5

4

3

3

3

3

3

3

1

1

1

1

1
This MEK2 antibody is un-conjugated
Application
108

60

36

22

20

20

18

14

13

10

7

3

3

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity

Human, Mouse

Predicted Reactivity

Rat,Chicken

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human MAPKK2/MEK2

Isotype

IgG
anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 44-313) antibody
MAP2K2 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 200-229) antibody
MAP2K2 Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB12322 unconjugated

anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 262-292) antibody
MAP2K2 Reactivity: Human, Mouse WB, IF, IHC (p) Host: Rabbit Polyclonal RB3597 unconjugated

anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 1-30), (N-Term) antibody
MAP2K2 Reactivity: Human, Mouse, Rat WB, IF Host: Rabbit Polyclonal RB3595 unconjugated

anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 1-400) antibody
MAP2K2 Reactivity: Human WB, IP, IF Host: Rabbit Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 1-360) antibody
MAP2K2 Reactivity: Human WB, ELISA, IF Host: Mouse Monoclonal 8D10 unconjugated

anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 1-100) antibody
KO Validated MAP2K2 Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 264-400) antibody
MAP2K2 Reactivity: Human WB, IHC, ELISA, IF Host: Rabbit Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (C-Term) antibody
MAP2K2 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IP, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (pThr394) antibody
MAP2K2 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IP, IF, ICC Host: Rabbit Polyclonal unconjugated

Application Notes

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions

For Research Use only
Validation #029748 (Western Blotting)

by

Alamo Laboratories Inc

No.

#029748

Date

07/02/2014

Antigen

Lot Number

110511

Method validated

Western Blotting

Positive Control

Hela cell extract

Negative Control

c6/36 Mosquito cell extract (non-reactive species)

Notes

A single positive band at the correct molecular weight was detected in positive control HeLa cell extract. Several bands were observed in the non-reactive species negative control at approximately the same molecular weight as MAP2K2. These bands may constitute cross-reactivity of the target antigen, or they may be non-specific bands.

Validation Images

Figure 1. Western blot of lysates from HeLa cells (Lane 1) and c6/36 cells (Lane 2) probed with anti-MAP2K2 (upper panel) or with anti-Actin for loading control (lower panel).
Full Methods
Primary Antibody

Antigen: Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (1:150 dilution)
Catalog number: ABIN726500
Lot number: 110511

Secondary Antibody

Antibody: Goat Anti-Rabbit IgG (H + L)-HRP Conjugate (1:20,000 dilution)
Lot number: L170-6515

Full Protocol

1. Total protein extracts were boiled in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol at 95°C for 5 min prior to loading.
2. 46 μg of boiled extracts were loaded and resolved on 8-16% SDS-polyacrylamide gel.
3. The Thermo Scientific - Spectra Multicolor Broad Range (Cat # 26634) were used as molecular mass markers.
4. Proteins were then transferred onto PVDF membrane by wet transfer and protein transfer was confirmed with Ponceau-S staining.
5. The PVDF membrane was incubated with 25 mL of blocking buffer [Tris Buffered Saline, pH 7.4 plus 0.1% TW20 (TBST)] containing 5% (W/V) non-fat dry milk at room temperature for 1 h.
6. The membrane was rinsed with TBST once.
7. The membrane was immersed with the protein side up in the primary antibody solution (anti-MAP2K2; 1:150) in TBST containing 5% (W/V) non-fat dry milk and incubated for 16 h at 4°C.
8. The membrane was rinsed in TBST thrice for 5 min each.
9. The membrane was incubated in the HRP-conjugated secondary antibody solution (Goat anti-rabbit IgG-HRP; 1:20,000) in TBST containing 5% (W/V) non-fat dry milk and incubated for 1 h at room temperature (~26°C) with gentle agitation.
10. The membrane was rinsed thrice TBST thrice for 5 min each.
11. The membrane was rinsed in TBS twice for 30 s each.
12. Signals were detected with ECL-2 Substrate. The blot was scanned for 300 s.
13. The membrane was rinsed three times TBST.
14. Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 min each.
15. The membrane was washed in TBST 2 times for 10 min each.
16. Repeated Steps 5-12 with the loading control antibody (anti-Actin; 1:6,000) and its matching secondary antibody (Goat anti-rabbit IgG-HRP; 1:20,000).

Experimental Notes

- No challenges noted.
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Target

MEK2 (MAP2K2) (Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2))

Alternative Name

MEK2 (MAP2K2 Products)

Synonyms

CFC4 antibody, MAPKK2 antibody, MEK2 antibody, MKK2 antibody, PRKMK2 antibody, AA589381 antibody, MK2 antibody, Prkmk2 antibody, ATMKK2 antibody, F27B13.50 antibody, F27B13_50 antibody, MAP KINASE KINASE 1 antibody, MAP kinase kinase 2 antibody, MK1 antibody, map2k2 antibody, wu:fa56c06 antibody, zgc:123171 antibody, zgc:172250 antibody, mitogen-activated protein kinase kinase 2 antibody, mitogen activated protein kinase kinase 2 antibody, MAP kinase kinase 2 antibody, mitogen-activated protein kinase kinase 2a antibody, mitogen-activated protein kinase kinase 2b antibody, MAP2K2 antibody, Map2k2 antibody, MKK2 antibody, map2k2a antibody, map2k2b antibody

Background

Synonyms: CFC4, MEK2, MKK2, MAPKK2, PRKMK2, Dual specificity mitogen-activated protein kinase kinase 2, MAP kinase kinase 2, MAPKK 2, ERK activator kinase 2, MAPK/ERK kinase 2, MEK 2, MAP2K2
Background: Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity).

Gene ID

5605

UniProt

P36507

Pathways

MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling