Reticulon 4 Protein (RTN4) (AA 27-447) (His tag)
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- Target See all Reticulon 4 (RTN4) Proteins
- Reticulon 4 (RTN4)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 27-447
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Reticulon 4 protein is labelled with His tag.
- Purpose
- Human NOGOR Protein
- Sequence
- Cys27-Ser447
- Characteristics
- Recombinant Human NOGOR Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Cys27-Ser447.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human NOGOR, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-NOGOR Antibody, hFc Tag with the EC50 of 9ng/ml determined by ELISA. See testing image for detail.
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Reticulon 4 (RTN4) (AA 27-447) protein (His tag)
RTN4 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
Reticulon 4 (RTN4) (AA 27-447) protein (His tag)RTN4 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Reticulon 4 (RTN4) (AA 1-185) protein (His tag)RTN4 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Reticulon 4 (RTN4) (AA 1-199) protein (GST tag)RTN4 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Reticulon 4 (RTN4) (AA 1-1192) protein (GST tag)RTN4 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Reticulon 4 (RTN4) (AA 1-1018) protein (His tag)Crystallography grade RTN4 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys
Reticulon 4 (RTN4) (AA 1-988) protein (His tag)Crystallography grade RTN4 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys
Reticulon 4 (RTN4) (AA 1-1192) protein (Strep Tag)Crystallography grade RTN4 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS
Reticulon 4 (RTN4) (AA 1-1162) protein (Strep Tag)Crystallography grade RTN4 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- Reticulon 4 (RTN4)
- Alternative Name
- NOGOR (RTN4 Products)
- Synonyms
- ASY Protein, NI220/250 Protein, NOGO Protein, NOGO-A Protein, NOGOC Protein, NSP Protein, NSP-CL Protein, Nbla00271 Protein, Nbla10545 Protein, Nogo-B Protein, Nogo-C Protein, RTN-X Protein, RTN4-A Protein, RTN4-B1 Protein, RTN4-B2 Protein, RTN4-C Protein, RTN4-Cw Protein, DKFZp459C0314 Protein, 1110020G17Rik Protein, AA407876 Protein, AA409940 Protein, AA960376 Protein, C130026I10Rik Protein, NgA Protein, Nogo-A Protein, mKIAA0886 Protein, mKIAA4153 Protein, NI-250 Protein, Nogo Protein, Vp20 Protein, r Protein, rat N Protein, rat NogoA Protein, reticulon-4 Protein, nogo Protein, rtn4 Protein, wu:fb17a02 Protein, wu:fb22f12 Protein, wu:fd61a07 Protein, zgc:92163 Protein, reticulon 4 Protein, reticulon 4a Protein, RTN4 Protein, rtn4 Protein, Rtn4 Protein, rtn4a Protein
- Background
- NOGO Receptor 1 (RTN4R) regulates axonal growth, as well as axon regeneration after injury. The gene maps to the 22q11.2 schizophrenia susceptibility locus and is thus a strong functional and positional candidate gene.RTN4R may modulate the genetic risk or clinical expression of schizophrenia in a subset of patients and identify additional studies that will be necessary to clarify the role of RTN4R in psychiatric phenotypes.
- Molecular Weight
- 46.4 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
- UniProt
- Q9BZR6
- Pathways
- Neurotrophin Signaling Pathway, Regulation of Cell Size, SARS-CoV-2 Protein Interactome
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