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Patched 1 antibody (C-Term)

Name: Patched 1 antibody (C-Term)
Brand: antibodies-online
SKU: ABIN185521
Price: 469.28 USD
Availability: InStock

Verified PTCH1 Reactivity: Human, Mouse ELISA, IHC, IF Host: Goat Polyclonal unconjugated

ABIN185521 Negative Control showing staining of paraffin embedded Human Cortex, with no primary antibody.

IHC

ABIN185521 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.

ABIN185521 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.

IHC

ABIN185521 (8 μg/mL) staining of paraffin embedded Human Cortex.

IHC

7 images

(2 references)

Catalog No. ABIN185521

Datasheet as PDF

Target See all Patched 1 (PTCH1) Antibodies

Patched 1 (PTCH1)
Binding Specificity
5

4

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
C-Term
Reactivity
36

26

11

3

2

2

2

2

2

1

1
Human, Mouse
Host
33

5

3
Goat
Compatible Secondaries
Clonality
37

4
Polyclonal
Conjugate
29

2

2

2

1

1

1

1

1

1
This Patched 1 antibody is un-conjugated
Application
28

21

13

8

6

5

1

1
ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose

PTCH (C Terminus)

Sequence

ELQDVECEER PR

Specificity

This antibody is expected to recognise all reported isoforms. Variants (NP_001077073.1, NP_001077074.1, NP_001077075.1, NP_001077076.1) encode the same isoform.

Cross-Reactivity

Human, Mouse, Rat

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade

Verified

Immunogen

Peptide with sequence ELQDVECEERPR, from the C Terminus of the protein sequence according to NP_000255.1, NP_001077072.1, NP_001077071.1, NP_001077073.1, NP_001077074.1, NP_001077075.1, NP_001077076.1.

Isotype

IgG
anti-Patched 1 (PTCH1) antibody
PTCH1 Reactivity: Human, Mouse, Rat IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Patched 1 (PTCH1) (AA 213-422) antibody
PTCH1 Reactivity: Mouse WB, IHC, ICC, IP Host: Rabbit Polyclonal unconjugated

anti-Patched 1 (PTCH1) antibody
PTCH1 Reactivity: Human, Mouse, Rat WB, ELISA, IF, ICC, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Patched 1 (PTCH1) (N-Term) antibody
PTCH1 Reactivity: Human, Mouse WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Patched 1 (PTCH1) antibody
PTCH1 Reactivity: Human, Mouse WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-Patched 1 (PTCH1) (Internal Region) antibody
Verified PTCH1 Reactivity: Human, Mouse WB, ELISA, IF Host: Goat Polyclonal unconjugated

anti-Patched 1 (PTCH1) (AA 770-970) antibody
PTCH1 Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated

anti-Patched 1 (PTCH1) (C-Term) antibody
PTCH1 Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Dog, Cow, Horse, Guinea Pig, Rabbit WB, IHC Host: Rabbit Polyclonal unconjugated

anti-Patched 1 (PTCH1) (AA 1180-1411) antibody
PTCH1 Reactivity: Human ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Patched 1 (PTCH1) (AA 266-280) antibody
Verified PTCH1 Reactivity: Human, Mouse WB, ELISA, IHC, IF Host: Goat Polyclonal unconjugated

Application Notes

Immunohistochemistry: Paraffin embedded Human Brain (Coretx). Recommended concentration: 8 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:16000.

Comment

Immunofluorescence: Strong expression of the protein seen in the Golgi of HeLa and NIH3T3 cells. Recommended concentration: 10μg/ml.

Restrictions

For Research Use only
Format

Liquid

Concentration

0.5 mg/mL

Buffer

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Minimize freezing and thawing.

Storage

-20 °C

Storage Comment

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Abiria, Williams, Munden, Grover, Wallace, Lundberg, Valadez, Cooper: "Expression of Hedgehog ligand and signal transduction components in mutually distinct isocitrate dehydrogenase mutant glioma cells supports a role for paracrine signaling." in: Journal of neuro-oncology, Vol. 119, Issue 2, pp. 243-51, (2014) (PubMed).

Gerardo Valadez, Grover, Carter, Calcutt, Abiria, Lundberg, Williams, Cooper: "Identification of Hedgehog pathway responsive glioblastomas by isocitrate dehydrogenase mutation." in: Cancer letters, Vol. 328, Issue 2, pp. 297-306, (2012) (PubMed).
Target

Patched 1 (PTCH1)

Alternative Name

PTCH1 (PTCH1 Products)

Synonyms

BCNS antibody, HPE7 antibody, NBCCS antibody, PTC antibody, PTC1 antibody, PTCH antibody, PTCH11 antibody, lep antibody, ptc1 antibody, ptc2 antibody, Ptch antibody, Ptch2 antibody, Ptc-1 antibody, XPtc1 antibody, patched antibody, patched1 antibody, A230106A15Rik antibody, Ptc antibody, Ptc1 antibody, mes antibody, patched 1 antibody, patched 1 L homeolog antibody, Protein patched homolog 1 antibody, PTCH1 antibody, ptch1 antibody, Ptch1 antibody, ptch1.L antibody, ptc-1 antibody

Background

PTCH1, PTCH, patched homolog (Drosophila), HGNC:9585, BCNS, HPE7, NBCCS, PTC, PTC1, patched, patched (Drosophila) homolog, patched homolog 1 (Drosophila), RP11-435O5.3, FLJ26746, FLJ42602, PTCH11, OTTHUMP00000021709, OTTHUMP00000021710, PTCH protein +12b,

Gene ID

5727

NCBI Accession

NP_000255, NP_001077072, NP_001077071, NP_001077073, NP_001077074, NP_001077075, NP_001077076

Pathways

Hedgehog Signaling, Carbohydrate Homeostasis, Tube Formation