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PTGS2 antibody (AA 501-604)

Name: PTGS2 antibody (AA 501-604)
SKU: ABIN672471
Price: 384.62 USD
Availability: InStock

PTGS2 Reactivity: Human, Mouse, Rat WB, ELISA, IHC (p), IF (cc), IF (p), FACS, IHC (fro) Host: Rabbit Polyclonal unconjugated

Mouse brain lysates probed with Rabbit Anti-Cyclooxygenase 2 Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti Cyclooxygenase 2/COX2 Polyclonal Antibody, Unconjugated (ABIN672471) at 1:200 followed by conjugation to the secondary antibody and DAB staining

IHC

ELISA

Human A549 lysates probed with Rabbit Anti-Cyclooxygenase 2 Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti Cyclooxygenase 2/COX2 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

IHC (p)

ELISA

Effects of Celecoxib on COX-2 and cyclin D1 activation in SKOV-3 xenograft tumors: (A) Western blot analysis of SKOV-3 tumors treated with a high-dose of Celecoxib (100 mg/kg).

COX protein levels in xenograft tumors of nude mice treated or not treated with combined treatment of SC-560 and celecoxib.

COX-2 over expressed in MDR-Caco-2 cells, which could be reversed by sinomenine.

9 images

(14 references)

Catalog No. ABIN672471

Datasheet as PDF

Target See all PTGS2 Antibodies

PTGS2 (Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2))
Binding Specificity
15

15

12

7

7

6

6

6

5

4

4

4

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
AA 501-604
Reactivity
103

56

43

21

5

2

2

1

1
Human, Mouse, Rat
Host
94

19

2
Rabbit
Compatible Secondaries
Clonality
88

27
Polyclonal
Conjugate
64

9

8

5

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1
This PTGS2 antibody is un-conjugated
Application
101

40

36

30

26

25

22

18

14

14

7

3

3

2

2

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity

Human, Mouse, Rat

Predicted Reactivity

Dog,Cow,Sheep,Pig,Rabbit

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from mouse Cyclooxygenase 2

Isotype

IgG
anti-Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2) (AA 187-425) antibody
PTGS2 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2) (AA 240-351) antibody
PTGS2 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2) (AA 375-604) antibody
PTGS2 Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2) (AA 418-589) antibody
PTGS2 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2) (AA 363-391) antibody
PTGS2 Reactivity: Human WB, IF, IHC (p) Host: Rabbit Polyclonal RB17409 unconjugated

anti-Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2) (C-Term) antibody
Verified PTGS2 Reactivity: Human, Mouse WB, ELISA, IF, FACS Host: Goat Polyclonal unconjugated

anti-Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2) antibody
PTGS2 Reactivity: Human WB, IHC, ELISA, IF Host: Rabbit Polyclonal unconjugated

anti-Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2) (AA 530-610) antibody
PTGS2 Reactivity: Human WB, ELISA, IF, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2) (AA 301-400) antibody
PTGS2 Reactivity: Human, Mouse, Rat, Sheep WB, ELISA, IHC (p), ICC, IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2) (AA 555-604) antibody
PTGS2 Reactivity: Human WB, IHC, ELISA, IF, IHC (p) Host: Rabbit Polyclonal unconjugated

Application Notes

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Kellermann, Janssen, Münch, Koch, Schneider-Stock, Cesnjevar, Rüffer: "Deep hypothermic circulatory arrest or tepid regional cerebral perfusion: impact on haemodynamics and myocardial integrity in a randomized experimental trial." in: Interactive cardiovascular and thoracic surgery, Vol. 26, Issue 4, pp. 667-672, (2018) (PubMed).

Chen, Hou, Fan, Jin, Wang: "Sonic hedgehog protein regulates fibroblast growth factor 8 expression in metanephric explant culture from BALB/c mice: Possible mechanisms associated with renal morphogenesis." in: Molecular medicine reports, Vol. 14, Issue 4, pp. 2929-36, (2017) (PubMed).

Hu, Xi, Cao, Yang, Liu, Sheng, Han, Yuan, Guo, Weng, Xu: "The expression of prostaglandin-E2 and its receptor in the oviduct of Chinese brown frog (Rana dybowskii)." in: Prostaglandins & other lipid mediators, Vol. 124, pp. 9-15, (2016) (PubMed).

Li, Xu, Jiang, Bi, Xu, Han: "Lipopolysaccharide induces inflammation and facilitates lung metastasis in a breast cancer model via the prostaglandin E2-EP2 pathway." in: Molecular medicine reports, Vol. 11, Issue 6, pp. 4454-62, (2015) (PubMed).

Liu, Duan, Chang, Zhang, Chu, Li, Dai, Mo, Chang: "Sinomenine sensitizes multidrug-resistant colon cancer cells (Caco-2) to doxorubicin by downregulation of MDR-1 expression." in: PLoS ONE, Vol. 9, Issue 6, pp. e98560, (2015) (PubMed).

Wallace, Zheng, Kanazawa, Shih: "Immunopathology of inflammatory bowel disease." in: World journal of gastroenterology : WJG, Vol. 20, Issue 1, pp. 6-21, (2014) (PubMed).

Li, Li, Mu, Zhang, Yao, Li, Niu: "Protective effect of sanguinarine on LPS-induced endotoxic shock in mice and its effect on LPS-induced COX-2 expression and COX-2 associated PGE2 release from peritoneal macrophages." in: International immunopharmacology, Vol. 22, Issue 2, pp. 311-7, (2014) (PubMed).

Zhang, Wu, Wan, Song, Xu, Xu, Chang, Tai, Dong, Liu: "Protective role of hydrogen-rich water on aspirin-induced gastric mucosal damage in rats." in: World journal of gastroenterology : WJG, Vol. 20, Issue 6, pp. 1614-22, (2014) (PubMed).

de Moraes, Maia, de Lima, Dias, Raposo-Ferreira, Sudano, Junior, Oba et al.: "Temporal analysis of prostaglandin F2? receptor, caspase 3, and cyclooxygenase 2 messenger RNA expression and prostaglandin F2? receptor and cyclooxygenase 2 protein expression in endometrial tissue ..." in: Theriogenology, Vol. 83, Issue 2, pp. 276-84, (2014) (PubMed).

Gao, Wen, Yang, Lu, Tong, Huang, Liu, Tang: "Celecoxib ameliorates portal hypertension of the cirrhotic rats through the dual inhibitory effects on the intrahepatic fibrosis and angiogenesis." in: PLoS ONE, Vol. 8, Issue 7, pp. e69309, (2013) (PubMed).

Chu, He, Kuang, Ren, Gou: "Celastrol protects kidney against ischemia-reperfusion-induced injury in rats." in: The Journal of surgical research, Vol. 186, Issue 1, pp. 398-407, (2013) (PubMed).

Li, Wang, Jiang, Xu, Zhang, Liu, Zhai: "Combined effects of cyclooxygenase-1 and cyclooxygenase-2 selective inhibitors on ovarian carcinoma in vivo." in: International journal of molecular sciences, Vol. 12, Issue 1, pp. 668-81, (2011) (PubMed).

Li, Jiang, Xu, Wang, Zhang, Liu, Zhai: "Cyclin D1 expression and the inhibitory effect of celecoxib on ovarian tumor growth in vivo." in: International journal of molecular sciences, Vol. 11, Issue 10, pp. 3999-4013, (2010) (PubMed).
Target

PTGS2 (Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2))

Alternative Name

Cyclooxygenase 2 (PTGS2 Products)

Synonyms

COX-2 antibody, COX2 antibody, GRIPGHS antibody, PGG/HS antibody, PGHS-2 antibody, PHS-2 antibody, hCox-2 antibody, Cox-2 antibody, Pghs2 antibody, TIS10 antibody, Cox2 antibody, cox-2 antibody, CEF147 antibody, PGHS2 antibody, PHSII antibody, cox2 antibody, gripghs antibody, pgg/hs antibody, pghs-2 antibody, phs-2 antibody, PTGS2 antibody, hcox-2 antibody, fj02a10 antibody, ptgs2 antibody, unp1239 antibody, wu:fj02a10 antibody, zCOX-2 antibody, si:dkey-97o5.6 antibody, prostaglandin-endoperoxide synthase 2 antibody, prostaglandin G/H synthase 2 antibody, prostaglandin-endoperoxide synthase 2S homeolog antibody, prostaglandin-endoperoxide synthase 2a antibody, prostaglandin-endoperoxide synthase 2b antibody, PTGS2 antibody, Ptgs2 antibody, LOC100136456 antibody, ptgs2.S antibody, ptgs2 antibody, ptgs2a antibody, ptgs2b antibody

Background

Synonyms: COX2, Cox-2, PHS-2, Pghs2, TIS1, PGHS-2, Prostaglandin G/H synthase 2, Cyclooxygenase-2, Glucocorticoid-regulated inflammatory cyclooxygenase, Gripghs, Macrophage activation-associated marker protein P71/73, PES-2, PHS II, Prostaglandin H2 synthase 2, PGH synthase 2, Prostaglandin-endoperoxide synthase 2, TIS1 protein, Ptgs2, Pghs-b
Background: Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and in pathological conditions, such as in cancer. PTGS2 is responsible for production of inflammatory prostaglandins. Up-regulation of PTGS2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, PTGS2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis.

Gene ID

19225

UniProt

Q05769

Pathways

Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation