For best experience we recommend to activate Javascript in your browser.

CD45 antibody (AA 1210-1304)

Name: CD45 antibody (AA 1210-1304)
SKU: ABIN669606
Price: 384.62 USD
Availability: InStock

PTPRC Reactivity: Human, Mouse, Rat, Rabbit FACS, WB, IHC (p), ELISA, IF (p), IF (cc) Host: Rabbit Polyclonal unconjugated

Raji cells probed with CD45 Polyclonal Antibody, Unconjugated at 1:20 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

FACS

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-CD45/B220 Polyclonal Antibody, Unconjugated (ABIN669606) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

IHC

Formalin-fixed and paraffin embedded human gastric adenocarcinoma labeled with Rabbit Anti-CD45/B220 Polyclonal Antibody, Unconjugated (ABIN669606) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

IHC

Formalin-fixed and paraffin embedded mouse lung labeled with Anti-CD45 Polyclonal Antibody, Unconjugated (ABIN669606) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, PE conjugated used at 1:200 dilution for 40 minutes at 37°C

Lane 1: Mouse spleen lysates; Lane 2: Human raji lysates probed with Rabbit Anti-CD45 Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Formalin-fixed and paraffin embedded human gastric adenocarcinoma labeled with Rabbit Anti-CD45/B220 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.

IHC (p)

6 images

(6 references)

Catalog No. ABIN669606

Datasheet as PDF

Target See all CD45 (PTPRC) Antibodies

CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
Binding Specificity
18

16

15

14

13

11

11

9

7

7

6

4

3

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 1210-1304
Reactivity
392

226

78

29

19

16

15

10

7

6

6

4

4

3

3

3

2

1

1

1

1

1

1
Human, Mouse, Rat, Rabbit
Host
282

178

146

2

1

1
Rabbit
Compatible Secondaries
Clonality
466

136

1
Polyclonal
Conjugate
173

60

51

48

39

15

13

11

10

10

9

9

8

8

8

7

6

6

6

6

5

5

5

4

4

4

4

4

4

4

4

4

4

4

3

3

3

3

3

3

2

2

2

2

2

2

1

1

1

1

1
This CD45 antibody is un-conjugated
Application
502

205

151

127

126

74

67

57

47

26

17

15

14

12

12

5

4

3

3

3

2

2

1

1

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Specificity

The antibody is predicted to work with Mouse and Rat samples, but with decreased sensitivity compared to human samples.

Cross-Reactivity

Human, Mouse, Rabbit, Rat

Predicted Reactivity

Cow

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human CD45

Isotype

IgG
anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) antibody
PTPRC Reactivity: Human, Dog FACS, IHC, WB, StM Host: Mouse Monoclonal 2B11-PD7-26 unconjugated

anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) (AA 193-575) antibody
PTPRC Reactivity: Human IHC, WB, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) (AA 371-556) antibody
PTPRC Reactivity: Mouse IHC, WB, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) antibody
PTPRC Reactivity: Human FACS, WB, IP, IHC (p), ICC Host: Mouse Monoclonal MEM-28 unconjugated

anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) (AA 24-575) antibody
PTPRC Reactivity: Human FACS, IHC, WB, IF, ELISA Host: Mouse Monoclonal 17H9D4 unconjugated

anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) (C-Term) antibody
PTPRC Reactivity: Human, Mouse, Rat, Dog, Chicken, Cow, Cat, Rabbit, Sheep, Pig, Guinea Pig, Goat, Horse, Monkey, Avian, Donkey, Hamster WB Host: Goat Polyclonal unconjugated

anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) (AA 378-577) antibody
PTPRC Reactivity: Human, Mouse, Rat FACS, IHC, WB, ELISA, ICC Host: Mouse Monoclonal 2H3E2 unconjugated

anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) (AA 928-989) antibody
PTPRC Reactivity: Human IHC, WB, ELISA Host: Mouse Monoclonal 5D3A3 unconjugated

anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) (AA 431-577) antibody
PTPRC Reactivity: Human FACS, IHC, WB, ELISA, ICC, Neut Host: Mouse Monoclonal 7C9F12 unconjugated

anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) antibody
PTPRC Reactivity: Human FACS, IHC, WB, IF, StM Host: Rabbit Monoclonal PTPRC-2877R unconjugated Recombinant Antibody

Application Notes

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Zhu, Meng, Wang, Wang, Zhang, Li, Li, Tan, Yang, Huang: "Effects of neuritin on the differentiation of bone marrow‑derived mesenchymal stem cells into neuron‑like cells." in: Molecular medicine reports, Vol. 16, Issue 3, pp. 3201-3207, (2018) (PubMed).

Liao, Zhong, Liu, Li, Ling, Zou: "Bone mesenchymal stem cells co-expressing VEGF and BMP-6 genes to combat avascular necrosis of the femoral head." in: Experimental and therapeutic medicine, Vol. 15, Issue 1, pp. 954-962, (2018) (PubMed).

Tsang, Huang, Yang, Chi, Chiang, Wang, Andocs, Szasz, Li, Chi: "Improving immunological tumor microenvironment using electro-hyperthermia followed by dendritic cell immunotherapy." in: BMC cancer, Vol. 15, pp. 708, (2015) (PubMed).

Gao, Zhang, Ding, Chen, Hu, Jiang, Li, Chen, Wang, Ye, Zhu: "Common expression of stemness molecular markers and early cardiac transcription factors in human Wharton's jelly-derived mesenchymal stem cells and embryonic stem cells." in: Cell transplantation, Vol. 22, Issue 10, pp. 1883-900, (2014) (PubMed).

Zeng, Hu, Yuan, Hu, Zhong, Kang: "Increased expression of importin13 in endometriosis and endometrial carcinoma." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 18, Issue 6, pp. CR361-7, (2012) (PubMed).
Target

CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Alternative Name

CD45 (PTPRC Products)

Synonyms

PTPRC antibody, si:dkeyp-47c5.1 antibody, B220 antibody, CD45 antibody, CD45R antibody, GP180 antibody, L-CA antibody, LCA antibody, LY5 antibody, T200 antibody, Cd45 antibody, Ly-5 antibody, Lyt-4 antibody, loc antibody, Lca antibody, RT7 antibody, cd45 antibody, protein tyrosine phosphatase, receptor type C antibody, protein tyrosine phosphatase, receptor type, C antibody, receptor-type tyrosine-protein phosphatase C antibody, PTPRC antibody, ptprc antibody, LOC100074271 antibody, Ptprc antibody

Background

Synonyms: LCA, LY5, B220, CD45, L-CA, T2, CD45R, GP18, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, PTPRC
Background: Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).

Gene ID

5788

UniProt

P08575

Pathways

TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling