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RAD21 antibody (Center)

RAD21 Reactivity: Human, Mouse WB, IF, IP, IHC (p), ICC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN2856242
  • Target See all RAD21 Antibodies
    RAD21 (RAD21 Homolog (RAD21))
    Binding Specificity
    • 16
    • 11
    • 4
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Center
    Reactivity
    • 56
    • 30
    • 29
    • 6
    • 5
    • 5
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    Human, Mouse
    Host
    • 48
    • 6
    • 1
    Rabbit
    Clonality
    • 44
    • 12
    Polyclonal
    Conjugate
    • 34
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This RAD21 antibody is un-conjugated
    Application
    • 48
    • 17
    • 13
    • 13
    • 11
    • 10
    • 9
    • 6
    • 6
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
    Characteristics
    Rabbit Polyclonal antibody to RAD21 (RAD21 homolog (S. pombe))
    RAD21 antibody
    Purification
    Purified by antigen-affinity chromatography.
    Immunogen
    Recombinant protein encompassing a sequence within the center region of human RAD21. The exact sequence is proprietary.
    Isotype
    IgG
  • Application Notes
    Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*
    Comment

    Positive Control: K562 , THP-1 , HL-60 , NIH-3T3 , JC , BCL-1

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Ochiai, Hayashi, Umeda, Yoshimura, Harada, Shimizu, Nakano, Saitoh, Liu, Yamamoto, Okamura, Ohkawa, Kimura, Nikaido: "Genome-wide kinetic properties of transcriptional bursting in mouse embryonic stem cells." in: Science advances, Vol. 6, Issue 25, pp. eaaz6699, (2020) (PubMed).

  • Target
    RAD21 (RAD21 Homolog (RAD21))
    Alternative Name
    RAD21 (RAD21 Products)
    Synonyms
    rad21 antibody, wu:fb55d02 antibody, wu:fb93f04 antibody, CDLS4 antibody, HR21 antibody, HRAD21 antibody, MCD1 antibody, NXP1 antibody, SCC1 antibody, hHR21 antibody, mKIAA0078 antibody, hr21 antibody, mcd1 antibody, nxp-1 antibody, nxp1 antibody, scc1 antibody, xrad21 antibody, RAD21 cohesin complex component antibody, RAD21 cohesin complex component a antibody, RAD21 cohesin complex component L homeolog antibody, RAD21 cohesin complex component S homeolog antibody, R-spondin 2 antibody, RAD21 antibody, rad21a antibody, rad21.L antibody, Rad21 antibody, rad21.S antibody, RSPO2 antibody
    Background
    The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells.

    Cellular Localization: Nucleus
    Molecular Weight
    72 kDa
    Gene ID
    5885
    Pathways
    Positive Regulation of Endopeptidase Activity
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