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ROBO1 antibody (Internal Region)

Verified ROBO1 Reactivity: Human ELISA, IHC, IF Host: Goat Polyclonal unconjugated
Catalog No. ABIN185537
  • Target See all ROBO1 Antibodies
    ROBO1 (Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1))
    Binding Specificity
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 31
    • 14
    • 14
    • 2
    • 1
    Human
    Host
    • 27
    • 5
    • 1
    Goat
    Clonality
    • 29
    • 4
    Polyclonal
    Conjugate
    • 22
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This ROBO1 antibody is un-conjugated
    Application
    • 20
    • 16
    • 5
    • 5
    • 4
    • 3
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)
    Purpose
    ROBO1 / DUTT1 (Internal)
    Sequence
    GDVDLSNKIN EMK
    Specificity
    Not expected to cross-react with ROBO2 or ROBO3.
    Cross-Reactivity
    Cow, Dog, Human, Mouse, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-GDVDLSNKINEMK, from the internal region of the protein sequence according to NP_002932.1, NP_598334.1, NP_001139316.1, NP_001139317.1.
    Isotype
    IgG
  • Application Notes

    Immunohistochemistry: Paraffin embedded Human Brain (Cortex) and Kidney. Recommended concentration: 5-6 μg/mL.

    Peptide ELISA: antibody detection limit dilution 1:16000.

    Comment

    Immunofluorescence: Strong expression of the protein seen in the Plasma membrane and Golgi/ER of HeLa and U2OS cells. Recommended concentration: 10μg/ml.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    ROBO1 (Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1))
    Alternative Name
    ROBO1 (ROBO1 Products)
    Synonyms
    DUTT1 antibody, SAX3 antibody, AW494633 antibody, AW742721 antibody, Gm310 antibody, ROBO1 antibody, dutt1 antibody, roundabout antibody, sax3 antibody, MGC122264 antibody, roundabout-1 antibody, roundabout guidance receptor 1 antibody, roundabout, axon guidance receptor, homolog 1 (Drosophila) antibody, roundabout guidance receptor 1 L homeolog antibody, ROBO1 antibody, Robo1 antibody, robo1 antibody, robo1.L antibody
    Background
    ROBO1, DUTT1, roundabout, axon guidance receptor, homolog 1 (Drosophila) , HGNC:10249, FLJ21882, SAX3, axon guidance receptor, roundabout 1, MGC131599, MGC133277
    Gene ID
    6091, 19876, 58946
    NCBI Accession
    NP_002932, NP_598334
    Pathways
    Positive Regulation of Endopeptidase Activity
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