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MRPL12 antibody (AA 1-198)

Name: MRPL12 antibody (AA 1-198)
SKU: ABIN562710
Price: 611.43 USD
Availability: InStock

MRPL12 Reactivity: Human WB, ELISA, IF, IHC (p), RNAi Host: Mouse Monoclonal 3B12-1A3 unconjugated

Western Blot detection against Immunogen (47.

Immunoperoxidase of monoclonal antibody to MRPL12 on formalin-fixed paraffin-embedded human breast cancer tissue.

ISt

Immunofluorescence of monoclonal antibody to MRPL12 on HeLa cell.

MRPL12 monoclonal antibody (M01), clone 3B12-1A3 Western Blot analysis of MRPL12 expression in COLO 320 HSR .

Western blot analysis of MRPL12 over-expressed 293 cell line, cotransfected with MRPL12 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1).

Detection limit for recombinant GST tagged MRPL12 is approximately 0.

ELISA

Western Blot analysis of MRPL12 expression in transfected 293T cell line by MRPL12 monoclonal antibody (M01), clone 3B12-1A3.

7 images

(3 references)

Catalog No. ABIN562710

Datasheet as PDF

Target See all MRPL12 Antibodies

MRPL12 (Mitochondrial Ribosomal Protein L12 (MRPL12))
Binding Specificity
15

3

3

1

1

1

1

1

1

1
AA 1-198
Reactivity
34

12

7

2

1

1

1

1
Human
Host
32

2
Mouse
Compatible Secondaries
Clonality
33

1
Monoclonal
Conjugate
20

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This MRPL12 antibody is un-conjugated
Application
18

13

13

12

7

5

5

3

2

2

1

1
Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), RNA Interference (RNAi)

Purpose

Mouse monoclonal antibody raised against a full length recombinant MRPL12.

Sequence

MLPAAARPLW GPCLGLRAAA FRLARRQVPC VCAVRHMRSS GHQRCEALAG APLDNAPKEY PPKIQQLVQD IASLTLLEIS DLNELLKKTL KIQDVGLVPM GGVMSGAVPA AAAQEAVEED IPIAKERTHF TVRLTEAKPV DKVKLIKEIK NYIQGINLVQ AKKLVESLPQ EIKANVAKAE AEKIKAALEA VGGTVVLE

Cross-Reactivity

Human

Characteristics

Antibody Reactive Against Recombinant Protein.

Immunogen

MRPL12 (AAH02344, 1 a.a. ~ 198 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone

3B12-1A3

Isotype

IgG1
anti-Mitochondrial Ribosomal Protein L12 (MRPL12) (AA 1-198) antibody
MRPL12 Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated

anti-Mitochondrial Ribosomal Protein L12 (MRPL12) antibody
MRPL12 Reactivity: Human, Mouse, Rat WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Mitochondrial Ribosomal Protein L12 (MRPL12) (AA 1-198) antibody
MRPL12 Reactivity: Human ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-Mitochondrial Ribosomal Protein L12 (MRPL12) (Internal Region) antibody
MRPL12 Reactivity: Human, Mouse WB, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Mitochondrial Ribosomal Protein L12 (MRPL12) (AA 87-136) antibody
MRPL12 Reactivity: Human WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Mitochondrial Ribosomal Protein L12 (MRPL12) (Internal Region) antibody
MRPL12 Reactivity: Human, Mouse WB, ELISA Host: Rabbit Polyclonal unconjugated

anti-Mitochondrial Ribosomal Protein L12 (MRPL12) antibody
MRPL12 Reactivity: Human, Mouse, Rat WB Host: Rabbit Polyclonal unconjugated

anti-Mitochondrial Ribosomal Protein L12 (MRPL12) (AA 119-168) antibody
MRPL12 Reactivity: Human, Mouse, Rat WB Host: Rabbit Polyclonal unconjugated

anti-Mitochondrial Ribosomal Protein L12 (MRPL12) (AA 1-198) antibody
MRPL12 Reactivity: Human WB Host: Rabbit Polyclonal unconjugated

anti-Mitochondrial Ribosomal Protein L12 (MRPL12) (AA 1-80), (Internal Region) antibody
MRPL12 Reactivity: Human, Mouse WB, ELISA Host: Rabbit Polyclonal unconjugated

Application Notes

Optimal working dilution should be determined by the investigator.

Restrictions

For Research Use only
Buffer

In 1x PBS, pH 7.4

Handling Advice

Aliquot to avoid repeated freezing and thawing.

Storage

-20 °C

Storage Comment

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Richman, Davies, Shearwood, Ermer, Scott, Hibbs, Rackham, Filipovska: "A bifunctional protein regulates mitochondrial protein synthesis." in: Nucleic acids research, Vol. 42, Issue 9, pp. 5483-94, (2014) (PubMed).

Chen, Cairns, Papandreou, Koong, Denko: "Oxygen consumption can regulate the growth of tumors, a new perspective on the Warburg effect." in: PLoS ONE, Vol. 4, Issue 9, pp. e7033, (2009) (PubMed).

Wang, Cotney, Shadel: "Human mitochondrial ribosomal protein MRPL12 interacts directly with mitochondrial RNA polymerase to modulate mitochondrial gene expression." in: The Journal of biological chemistry, Vol. 282, Issue 17, pp. 12610-8, (2007) (PubMed).
Target

MRPL12 (Mitochondrial Ribosomal Protein L12 (MRPL12))

Alternative Name

MRPL12 (MRPL12 Products)

Synonyms

5c5-2 antibody, L12mt antibody, MRP-L31/34 antibody, MRPL7 antibody, MRPL7/L12 antibody, RPML12 antibody, GB14723 antibody, MRPL12 antibody, 0610034O11Rik antibody, 1500031N16Rik antibody, MRP-L12 antibody, Rpml12 antibody, Rpm12 antibody, im:6893415 antibody, zgc:112445 antibody, mrpl12 antibody, mitochondrial ribosomal protein L12 antibody, 39S ribosomal protein L12, mitochondrial antibody, mitochondrial nucleoid protein antibody, mitochondrial ribosomal protein L12 L homeolog antibody, MRPL12 antibody, LOC409926 antibody, CAALFM_C207190CA antibody, Mrpl12 antibody, mrpl12 antibody, mrpl12.L antibody

Background

Full Gene Name: mitochondrial ribosomal protein L12
Synonyms: 5c5-2,FLJ60124,L12mt,MGC8610,MRP-L31/34,MRPL7,MRPL7/L12,RPML12

Gene ID

6182