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MPP5 antibody (N-Term)

Verified MPP5 Reactivity: Human ELISA, IHC, IF, FACS Host: Goat Polyclonal unconjugated
Catalog No. ABIN185150
  • Target See all MPP5 Antibodies
    MPP5 (Membrane Protein, Palmitoylated 5 (MAGUK P55 Subfamily Member 5) (MPP5))
    Binding Specificity
    • 15
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivity
    • 39
    • 8
    • 7
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    Human
    Host
    • 34
    • 4
    • 2
    Goat
    Clonality
    • 37
    • 3
    Polyclonal
    Conjugate
    • 22
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This MPP5 antibody is un-conjugated
    Application
    • 34
    • 13
    • 13
    • 11
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
    Purpose
    PALS / MPP5
    Sequence
    NKASPPFPLI SNAQ
    Cross-Reactivity
    Cow, Dog, Human, Pig
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    NKASPPFPLISNAQ-C
    Isotype
    IgG
  • Application Notes

    Immunohistochemistry: Paraffin embedded Human Small Intestine and Kidney. Recommended concentration: 5 μg/mL.

    Western Blot: Preliminary testing showed a band at approx 75 kDa in Human Cerebellum lysate after 1 μg/mL antibody staining (calculated MW of 73.4 kDa according to NP_001243479.1). Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:32000.

    Comment

    Immunofluorescence: Strong expression of the protein seen in U2OS and HeLa cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    MPP5 (Membrane Protein, Palmitoylated 5 (MAGUK P55 Subfamily Member 5) (MPP5))
    Alternative Name
    MPP5 (MPP5 Products)
    Background
    MPP5, PALS1, FLJ12615, membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5), stardust (Drosophila), MAGUK p55 subfamily member 5, membrane protein, palmitoylated 5, stardust
    Gene ID
    64398
    NCBI Accession
    NP_071919
    Pathways
    Nucleotide Phosphorylation, Tube Formation, Asymmetric Protein Localization
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