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SGK1 antibody (pSer422)

Name: SGK1 antibody (pSer422)
SKU: ABIN6256329
Price: 390.77 USD
Availability: InStock

SGK1 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

Western blot analysis of SGK phosphorylation expression in Insulin treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

ABIN6267218 at 1/100 staining human skin tissue sections by IHC-P.

IHC

ABIN6267218 at 1/100 staining rat uterine tissue sections by IHC-P.

IHC

ABIN6267218 at 1/100 staining rat intestinal tissue sections by IHC-P.

13 images

Catalog No. ABIN6256329

Datasheet as PDF

Target See all SGK1 Antibodies

SGK1 (serum/glucocorticoid Regulated Kinase 1 (SGK1))
Binding Specificity
20

19

16

14

9

6

4

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pSer422
Reactivity
138

93

75

20

17

8

7

5

5

4

4

2

2

1

1
Human, Mouse, Rat
Host
123

11

4

1
Rabbit
Compatible Secondaries
Clonality
127

12
Polyclonal
Conjugate
66

9

8

7

5

4

4

4

4

4

4

4

4

4

3

3

2
This SGK1 antibody is un-conjugated
Application
131

56

50

44

40

29

27

21

9

7

3

3

2

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity

Phospho-SGK1 (Ser422) Antibody detects endogenous levels of SGK1 only when phosphorylated at Serine 422.

Predicted Reactivity

Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen

A synthesized peptide derived from human SGK1 around the phosphorylation site of Ser422.

Isotype

IgG
anti-serum/glucocorticoid Regulated Kinase 1 (SGK1) (AA 1-431) antibody
SGK1 Reactivity: Human WB, ELISA, IF, IHC (p), RNAi Host: Mouse Monoclonal 4D7-G3 unconjugated

anti-serum/glucocorticoid Regulated Kinase 1 (SGK1) (AA 172-431) antibody
SGK1 Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-serum/glucocorticoid Regulated Kinase 1 (SGK1) (AA 1-90) antibody
SGK1 Reactivity: Human WB, ELISA, IF, IHC (p) Host: Mouse Monoclonal 3G8 unconjugated

anti-serum/glucocorticoid Regulated Kinase 1 (SGK1) (AA 145-386) antibody
SGK1 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-serum/glucocorticoid Regulated Kinase 1 (SGK1) (AA 247-406) antibody
SGK1 Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-serum/glucocorticoid Regulated Kinase 1 (SGK1) (pSer78) antibody
SGK1 Reactivity: Human, Mouse, Rat WB, ELISA, IHC (p), IF (p), FACS, IF (cc), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-serum/glucocorticoid Regulated Kinase 1 (SGK1) (AA 1-90) antibody
SGK1 Reactivity: Human WB, ELISA, IF, IHC (p) Host: Mouse Monoclonal 3E3 unconjugated

anti-serum/glucocorticoid Regulated Kinase 1 (SGK1) (pSer422) antibody
SGK1 Reactivity: Human, Mouse, Rat WB, ELISA, IHC (p), IF (p), FACS, IF (cc), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-serum/glucocorticoid Regulated Kinase 1 (SGK1) (pThr256) antibody
SGK1 Reactivity: Human, Mouse WB, ELISA, IHC (p), IF (p), FACS, IF (cc), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-serum/glucocorticoid Regulated Kinase 1 (SGK1) (Tyr1062) antibody
SGK1 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

Application Notes

WB 1:500-1:2000, IHC 1:50-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions

For Research Use only
Format

Liquid

Concentration

1 mg/mL

Buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date

12 months
Target

SGK1 (serum/glucocorticoid Regulated Kinase 1 (SGK1))

Alternative Name

SGK1 (SGK1 Products)

Synonyms

SGK antibody, Sgk antibody, sgk-B antibody, sgk2 antibody, cb1083 antibody, sgk antibody, wu:fc20a09 antibody, sgk-A antibody, sgk1 antibody, sgk1-a antibody, sgk1-b antibody, serum/glucocorticoid regulated kinase 1 antibody, Serine/threonine-protein kinase sgk-1 antibody, serum/glucocorticoid regulated kinase 1 S homeolog antibody, serum/glucocorticoid regulated kinase 1 L homeolog antibody, SGK1 antibody, Sgk1 antibody, sgk-1 antibody, sgk1.S antibody, sgk1 antibody, sgk1.L antibody

Background

Description: Serine/threonine-protein kinase which is involved in the regulation of a wide variety of ion channels, membrane transporters, cellular enzymes, transcription factors, neuronal excitability, cell growth, proliferation, survival, migration and apoptosis. Plays an important role in cellular stress response. Contributes to regulation of renal Na+ retention, renal K+ elimination, salt appetite, gastric acid secretion, intestinal Na+/H+ exchange and nutrient transport, insulin-dependent salt sensitivity of blood pressure, salt sensitivity of peripheral glucose uptake, cardiac repolarization and memory consolidation. Up-regulates Na+ channels: SCNN1A/ENAC, SCN5A and ASIC1/ACCN2, K+ channels: KCNJ1/ROMK1, KCNA1-5, KCNQ1-5 and KCNE1, epithelial Ca2+ channels: TRPV5 and TRPV6, chloride channels: BSND, CLCN2 and CFTR, glutamate transporters: SLC1A3/EAAT1, SLC1A2 /EAAT2, SLC1A1/EAAT3, SLC1A6/EAAT4 and SLC1A7/EAAT5, amino acid transporters: SLC1A5/ASCT2, SLC38A1/SN1 and SLC6A19, creatine transporter: SLC6A8, Na+/dicarboxylate cotransporter: SLC13A2/NADC1, Na+-dependent phosphate cotransporter: SLC34A2/NAPI-2B, glutamate receptor: GRIK2/GLUR6. Up-regulates carriers: SLC9A3/NHE3, SLC12A1/NKCC2, SLC12A3/NCC, SLC5A3/SMIT, SLC2A1/GLUT1, SLC5A1/SGLT1 and SLC15A2/PEPT2. Regulates enzymes: GSK3A/B, PMM2 and Na+/K+ ATPase, and transcription factors: CTNNB1 and nuclear factor NF-kappa-B. Stimulates sodium transport into epithelial cells by enhancing the stability and expression of SCNN1A/ENAC. This is achieved by phosphorylating the NEDD4L ubiquitin E3 ligase, promoting its interaction with 14-3-3 proteins, thereby preventing it from binding to SCNN1A/ENAC and targeting it for degradation. Regulates store-operated Ca(+2) entry (SOCE) by stimulating ORAI1 and STIM1. Regulates KCNJ1/ROMK1 directly via its phosphorylation or indirectly via increased interaction with SLC9A3R2/NHERF2. Phosphorylates MDM2 and activates MDM2-dependent ubiquitination of p53/TP53. Phosphorylates MAPT/TAU and mediates microtubule depolymerization and neurite formation in hippocampal neurons. Phosphorylates SLC2A4/GLUT4 and up-regulates its activity. Phosphorylates APBB1/FE65 and promotes its localization to the nucleus. Phosphorylates MAPK1/ERK2 and activates it by enhancing its interaction with MAP2K1/MEK1 and MAP2K2/MEK2. Phosphorylates FBXW7 and plays an inhibitory role in the NOTCH1 signaling. Phosphorylates FOXO1 resulting in its relocalization from the nucleus to the cytoplasm. Phosphorylates FOXO3, promoting its exit from the nucleus and interference with FOXO3-dependent transcription. Phosphorylates BRAF and MAP3K3/MEKK3 and inhibits their activity. Phosphorylates SLC9A3/NHE3 in response to dexamethasone, resulting in its activation and increased localization at the cell membrane. Phosphorylates CREB1. Necessary for vascular remodeling during angiogenesis. Sustained high levels and activity may contribute to conditions such as hypertension and diabetic nephropathy. Isoform 2 exhibited a greater effect on cell plasma membrane expression of SCNN1A/ENAC and Na+ transport than isoform 1.
Gene: SGK1

Molecular Weight

54kDa

Gene ID

6446

UniProt

O00141

Pathways

MAPK Signaling, Notch Signaling, Steroid Hormone Mediated Signaling Pathway