Topoisomerase I antibody (Internal Region)
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- Target See all Topoisomerase I (TOP1) Antibodies
- Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))
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Binding Specificity
- Internal Region
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Topoisomerase I antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
- Specificity
- TOP1 Antibody detects endogenous levels of total TOP1.
- Predicted Reactivity
- Pig,Zebrafish,Horse,Rabbit,Dog,Chicken,Xenopus
- Purification
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- Immunogen
- A synthesized peptide derived from human TOP1, corresponding to a region within the internal amino acids.
- Isotype
- IgG
- Top Product
- Discover our top product TOP1 Primary Antibody
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anti-Topoisomerase (DNA) I (TOP1) (AA 692-765) antibody
TOP1 Reactivity: Human WB, ELISA, IHC (p) Host: Mouse Monoclonal 1A1 unconjugated
anti-Topoisomerase (DNA) I (TOP1) antibodyTOP1 Reactivity: Human WB, IHC, IF, IP Host: Rabbit Monoclonal unconjugated
anti-Topoisomerase (DNA) I (TOP1) antibodyTOP1 Reactivity: Human WB, ELISA, IHC, IP, FACS Host: Rabbit Monoclonal 6D8 unconjugated Recombinant Antibody
anti-Topoisomerase (DNA) I (TOP1) antibodyTOP1 Reactivity: Human, Mouse WB, IHC Host: Rabbit Polyclonal unconjugated
anti-Topoisomerase (DNA) I (TOP1) (N-Term) antibodyTOP1 Reactivity: Human WB, FACS Host: Mouse Monoclonal 1291CT875-142-166 unconjugated
anti-Topoisomerase (DNA) I (TOP1) (AA 1-100) antibodyTOP1 Reactivity: Human WB, IHC, IP Host: Rabbit Polyclonal unconjugated
anti-Topoisomerase (DNA) I (TOP1) (AA 2-31), (N-Term) antibodyTOP1 Reactivity: Human WB, IF Host: Rabbit Polyclonal RB34034 unconjugated
anti-Topoisomerase (DNA) I (TOP1) (AA 358-501) antibodyTOP1 Reactivity: Human ELISA, IF Host: Rabbit Polyclonal unconjugated
anti-Topoisomerase (DNA) I (TOP1) antibodyTOP1 Reactivity: Human, Rat WB, FACS Host: Mouse Monoclonal 1C6 unconjugated
anti-Topoisomerase (DNA) I (TOP1) (AA 692-765) antibodyTOP1 Reactivity: Human WB, ELISA Host: Mouse Polyclonal unconjugated
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- Application Notes
- WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20 °C. Stable for 12 months from date of receipt.
- Expiry Date
- 12 months
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- Target
- Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))
- Alternative Name
- TOP1 (TOP1 Products)
- Synonyms
- CG6146 antibody, Dmel\\CG6146 antibody, TOP1 antibody, TopI antibody, Topo I antibody, TopoI antibody, dTopoI antibody, l(1)G0134 antibody, l(1)G0201 antibody, l(1)G0229 antibody, l(1)G0278 antibody, l(1)top1 antibody, top1 antibody, topo I antibody, topoI antibody, DNA topoisomerase 1 beta antibody, DNA topoisomerase I alpha antibody, MGO1 antibody, MGOUN 1 antibody, MTE17.1 antibody, MTE17_1 antibody, TOP1BETA antibody, TOPOISOMERASE 1 antibody, TOPI antibody, AI467334 antibody, D130064I21Rik antibody, Top-1 antibody, Ab2-086 antibody, topi antibody, Topoisomerase 1 antibody, DNA topoisomerase I antibody, DNA topoisomerase I alpha antibody, DNA topoisomerase 1 antibody, topoisomerase (DNA) I antibody, DNA topoisomerase I, gene 1 L homeolog antibody, Top1 antibody, top1 antibody, topA antibody, ZMO1193 antibody, MRAD2831_RS63095 antibody, Lferr_0016 antibody, PC1_2015 antibody, Za10_0136 antibody, Gbro_0575 antibody, Dd586_2108 antibody, Kvar_3044 antibody, Alvin_2069 antibody, Dacet_0849 antibody, Mrub_1615 antibody, Arnit_2962 antibody, Ndas_4953 antibody, Trad_2377 antibody, Acear_1653 antibody, Mahau_0844 antibody, Mesop_4109 antibody, Thein_1586 antibody, Theth_0241 antibody, TOP1ALPHA antibody, top-1 antibody, TOP1 antibody, top1.1.L antibody
- Target Type
- Viral Protein
- Background
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Description: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter.
Gene: TOP1
- Molecular Weight
- 90 kDa
- Gene ID
- 7150
- UniProt
- P11387
- Pathways
- Caspase Cascade in Apoptosis, Stem Cell Maintenance
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